Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Solaris and Regent Grapevine Plants Treated with Bioproducts

The aim of this study was to identify the spores of arbuscular mycorrhizal fungi (AMF) in the rhizosphere of Solaris and Regent grapevine plants grafted onto SO4 rootstock and treated with bioproducts. The bioproducts Ausuma, Bioilsa, manure and BF Ekomix were tested and applied on their own, or combined with standard mineral fertilisation (NPK). The applied bioproducts contributed to an increase in the number of species of mycorrhizal fungi in the rhizosphere soil of the grapevines. The most frequently occurring AMF species was Claroideoglomus claroideum. Biopreparation BF Ekomix was a promisingagent for increasing the number of AMF spores in the rhizosphere of Regent grapevines. In the rhizosphere of Solaris, a positive influence on the number of spores occurred after the application of Bioilsa

On stationary Markov chains and independent random variables

Two new proofs are given for the fact that a stationary, irreducible, aperiodic Markov chain (Xn N = ..., -1,0,1,2...) with denumerable state space has a representation of the form X'n=g(Un-1, Un-2,...), where g is a measurable function, (Un, N= ..., -1,0,1,2,...) a sequence of independent random variables uniformly distributed on (0,1), and (X'n) has the same probability law as (Xn).Markov chain representation i.i.d. random variables

Microbial diversity as an indicator of a diversified cropping system for luvisoils in a moderate climate. Case study – Long term experiments from Poland

The idea of agricultural sustainable in the EU is based on, both minimizing interference with the soil system as well as diversifying crop rotation what relates to the limited cultivation system (changed from plow to no-plowing tillage) as well as organic fertilization is often abandoned. Taking above into account, our goal was determined of the structure, composition, and metabolic profiles of soil microbiomes in various cultivation methods (under multiannual plow and no-plow cultivation) using metagenomic analysis. Having regard to the recommendations contained in EU report (European Commission et al., 2020) of the Mission board for Soil health and food, 2020 indicating the lack of microbiological indicators of “healthy soil”. So, we have tried to select of microbiological indicators showing sensitivity and resistance to use the methods of soil cultivation. The research object was located on almost 100-year field experiments at the Experimental Station of the Faculty of Agriculture and Biology in Skierniewice/near Warsaw on, luvisoils dominated in the temperate climate of Central Europe. Soil microorganisms respond with changes in their abundance and taxonomic composition depending on the methods of soil cultivation. Actinobacteria were the most abundant, while Planctomycetes were the least abundant in the metagenome of soil fertilized with manure, whereas the uncultivated soil was dominated by Nitrospirae. We can recommend the following taxa, including Gemmatimonas sp. as a microbiological indicator sensitive to the long-term lack of both plow cultivation of soil and organic fertilization, and Mycobacterium sp. as a resistancivity indicator to this soil cultivation method. Sorangium sp. could be recommended as microbiological indicators which responds by reducing the quantity under effect of the organically fertilized soil, while the plow and no-plow cultivation does not affect changes in its quantity. The use of various cultivation methods changed the biochemical functions in soil metagenoms, including nitrogen and sulfur metabolism and carbohydrate metabolism, and in the production of plant hormones and siderophores. Additionally, soil cultivation ways changed the response of microorganism’s stresses, including oxidative stress. The conducted research indicates the necessity to conduct further research on the influence of various cultivation methods, on the diversity of the microorganism community and soil metabolism. The result of which may be the selection of appropriate microbiological indicators for determining “soil health” depending on the type of soil under cultivation located in different climatic zones, not only presented in the paper

Perennial flower strips for pest control in organic apple orchards

Orchards often have low diversity of plant species, which limits the promotion of natural enemies to combat the important apple pests Dysaphis plantaginea (Passerini) and Cydia pomonella (L.). We developed perennial, multifunctional flower strips with native plant species and trialed these in organic apple orchards in seven European countries. On average 73.7% of the sown plant species were established and plant diversity of flower strips was on average 43% higher than the spontaneous orchard vegetation. Intensive mulching of flower strips affected the plant community: species richness and ground cover by forbs and plants, which especially promote functional agrobiodiversity, decreased significantly. Visual assessments over two years revealed a higher number of natural enemies on plant parts, and specifically in D. plantaginea colonies, on trees in flower strip plots than on trees in control plots with standard orchard vegetation. Trees in the flower strip plots recorded a slower D. plantaginea population increase as compared with control plots, resulting in reduced fruit damage after the second fruit drop. Likewise, from 2016–2017, the number of preadult codling moths decreased more in the flower strip plots as compared to the control plots resulting in reduced fruit damage. Our study shows on a wide continental scale that the implementation of perennial flower strips in the alleyways between apple tree rows boosts natural enemies and reduces key apple pests and the associated fruit damage. This supports the role of functional agrobiodiversity as a way to potentially reduce insecticide use in orchards and thus further promote conservation of agrobiodiversity. The project “EcoOrchard” was provided by FP7 ERAnet, CORE Organic Plus and cofinanced by the European Commission

Proteasome machinery is instrumental in a common gain-of-function program of the p53 missense mutants in cancer

In cancer, the tumour suppressor gene TP53 undergoes frequent missense mutations that endow mutant p53 proteins with oncogenic properties. Until now, a universal mutant p53 gain-of-function program has not been defined. By means of multi-omics: proteome, DNA interactome (chromatin immunoprecipitation followed by sequencing) and transcriptome (RNA sequencing/microarray) analyses, we identified the proteasome machinery as a common target of p53 missense mutants. The mutant p53-proteasome axis globally affects protein homeostasis, inhibiting multiple tumour-suppressive pathways, including the anti-oncogenic KSRP-microRNA pathway. In cancer cells, p53 missense mutants cooperate with Nrf2 (NFE2L2) to activate proteasome gene transcription, resulting in resistance to the proteasome inhibitor carfilzomib. Combining the mutant p53-inactivating agent APR-246 (PRIMA-1MET) with the proteasome inhibitor carfilzomib is effective in overcoming chemoresistance in triple-negative breast cancer cells, creating a therapeutic opportunity for treatment of solid tumours and metastasis with mutant p53