Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor-2

<p><b>Copyright information:</b></p><p>Taken from "Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor"</p><p>Environmental Health Perspectives 2005;113(8):1009-1014.</p><p>Published online 15 Apr 2005</p><p>PMCID:PMC1280341.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor-5

<p><b>Copyright information:</b></p><p>Taken from "Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor"</p><p>Environmental Health Perspectives 2005;113(8):1009-1014.</p><p>Published online 15 Apr 2005</p><p>PMCID:PMC1280341.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor-6

<p><b>Copyright information:</b></p><p>Taken from "Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor"</p><p>Environmental Health Perspectives 2005;113(8):1009-1014.</p><p>Published online 15 Apr 2005</p><p>PMCID:PMC1280341.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor-8

<p><b>Copyright information:</b></p><p>Taken from "Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor"</p><p>Environmental Health Perspectives 2005;113(8):1009-1014.</p><p>Published online 15 Apr 2005</p><p>PMCID:PMC1280341.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor-7

<p><b>Copyright information:</b></p><p>Taken from "Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor"</p><p>Environmental Health Perspectives 2005;113(8):1009-1014.</p><p>Published online 15 Apr 2005</p><p>PMCID:PMC1280341.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor-3

<p><b>Copyright information:</b></p><p>Taken from "Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor"</p><p>Environmental Health Perspectives 2005;113(8):1009-1014.</p><p>Published online 15 Apr 2005</p><p>PMCID:PMC1280341.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor-0

<p><b>Copyright information:</b></p><p>Taken from "Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor"</p><p>Environmental Health Perspectives 2005;113(8):1009-1014.</p><p>Published online 15 Apr 2005</p><p>PMCID:PMC1280341.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor-4

<p><b>Copyright information:</b></p><p>Taken from "Pollutant Particles Produce Vasoconstriction and Enhance MAPK Signaling via Angiotensin Type I Receptor"</p><p>Environmental Health Perspectives 2005;113(8):1009-1014.</p><p>Published online 15 Apr 2005</p><p>PMCID:PMC1280341.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.</p

Ex Vivo Chemical Cytometric Analysis of Protein Tyrosine Phosphatase Activity in Single Human Airway Epithelial Cells

We describe a novel method for the measurement of protein tyrosine phosphatase (PTP) activity in single human airway epithelial cells (hAECs) using capillary electrophoresis. This technique involved the microinjection of a fluorescent phosphopeptide that is hydrolyzed specifically by PTPs. Analyses in BEAS-2B immortalized bronchial epithelial cells showed rapid PTP-mediated dephosphorylation of the substrate (2.2 pmol min^–1 mg^–1) that was blocked by pretreatment of the cells with the PTP inhibitors pervanadate, Zn²⁺, and 1,2-naphthoquinone (76%, 69%, and 100% inhibition relative to PTP activity in untreated controls, respectively). These studies were then extended to a more physiologically relevant model system: primary hAECs cultured from bronchial brushings of living human subjects. In primary hAECs, dephosphorylation of the substrate occurred at a rate of 2.2 pmol min^–1 mg^–1 and was also effectively inhibited by preincubation of the cells with the inhibitors pervanadate, Zn²⁺, and 1,2-naphthoquinone (91%, 88%, and 87% median PTP inhibition, respectively). Reporter proteolysis in single BEAS-2B cells occurred at a median rate of 43 fmol min^–1 mg^–1 resulting in a mean half-life of 20 min. The reporter displayed a similar median half-life of 28 min in these single primary cells. Finally, single viable epithelial cells (which were assayed for PTP activity immediately after collection by bronchial brushing of a human volunteer) showed dephosphorylation rates ranging from 0.34 to 36 pmol min^–1 mg^–1 (<i>n</i> = 6). These results demonstrate the utility and applicability of this technique for the ex vivo quantification of PTP activity in small, heterogeneous, human cells and tissues

Density Functional Theory at the Basis Set Limit with Multiwavelets

The mainstream approaches to represent orbitals in wave function theory and DFT are indubitably Gaussian Type Orbitals (GTOs) and plane waves (PWs) for isolated and periodic systems respectively. Such choices are inherited from a not so distant past when computational resources were much scarcer and it was mandatory to provide the most compact representation possible. Current computational resources open the way to real-space grid approaches such as multiwavelets. We show how, by making use of multiwavelets, unprecedented and -- most importantly -- controlled accuracy can be achieved for energy and properties. The approach is in principle also very well suited to harvest modern computational architectures, based on large distributed clusters. The main challenges for such an approach are represented by the memory requirements the "curse of dimensionality", which limit at present the approach to small systems (100 electrons or less) and single-determinant methods (HF and DFT). In this contribution we present briefly main ideas about the Multiwavelet approach and our recent results about energy and properties of molecules