A stochastic surrogate model for time-variant reliability analysis of flexible multibody system

The dynamic model of the flexible multibody systems (FMS) is usually the differential equations with time-variant, high nonlinear and strong coupling characteristics. The traditional reliability models are inefficient to solve these problems. And the reliability model is poor in accuracy and computational efficiency. Based on this point, a new stochastic surrogate model for time-variant reliability analysis of FMS is proposed. Combined model order reduction with generalized polynomial chaos, the stochastic surrogate model is established and the statistical characteristics of system responses are obtained. The calculation method of kinematic time-variant reliability is given. Finally, the effectiveness of the method is verified by a rotating flexible beam. The results show that this method has high computational accuracy compared with Monte Carlo method

Wdr18 Is Required for Kupffer's Vesicle Formation and Regulation of Body Asymmetry in Zebrafish

Correct specification of the left-right (L-R) axis is important for organ morphogenesis. Conserved mechanisms involving cilia rotation inside node-like structures and asymmetric Nodal signaling in the lateral plate mesoderm (LPM), which are important symmetry-breaking events, have been intensively studied. In zebrafish, the clustering and migration of dorsal forerunner cells (DFCs) is critical for the formation of the Kuppfer's vesicle (KV). However, molecular events underlying DFC clustering and migration are less understood. The WD-repeat proteins function in a variety of biological processes, including cytoskeleton assembly, intracellular trafficking, mRNA splicing, transcriptional regulation and cell migration. However, little is known about the function of WD-repeat proteins in L-R asymmetry determination. Here, we report the identification and functional analyses of zebrafish wdr18, a novel gene that encodes a WD-repeat protein that is highly conserved among vertebrate species. wdr18 was identified from a Tol2 transposon-mediated enhancer trap screen. Follow-up analysis of wdr18 mRNA expression showed that it was detected in DFCs or the KV progenitor cells and later in the KV at early somitogenesis stages. Morpholino knockdown of wdr18 resulted in laterality defects in the visceral organs, which were preceded by the mis-expression of Nodal-related genes, including spaw and pitx2. Examination of morphants at earlier stages revealed that the KV had fewer and shorter cilia which are immotile and a smaller cavity. We further investigated the organization of DFCs in wdr18 morphant embryos using ntl and sox17 as specific markers and found that the clustering and migration of DFC was altered, leading to a disorganized KV. Finally, through a combination of wdr18 and itgb1b morpholino injections, we provided evidence that wdr18 and itgb1b genetically interact in the laterality determination process. Thus, we reveal a new and essential role for WD-repeat proteins in the determination and regulation of L-R asymmetry and propose a potential mechanism for wdr18 in the regulation of DFC clustering and migration and KV formation

Flame Retardant Behavior of Ternary Synergistic Systems in Rigid Polyurethane Foams

In order to explore flame retardant systems with higher efficiency in rigid polyurethane foams (RPUFs), aluminum hydroxide (ATH), [bis(2-hydroxyethyl)amino]-methyl-phosphonic acid dimethyl ester (BH) and expandable graphite (EG) were employed in RPUF for constructing ternary synergistic flame retardant systems. Compared with binary BH/EG systems and aluminum oxide (AO)/BH/EG, ATH/BH/EG with the same fractions in RPUFs demonstrated an increase in the limited oxygen index value, a decreased peak value of heat release rate, and a decreased mass loss rate. In particular, it inhibited smoke release. During combustion, ATH in ternary systems decomposed and released water, which captured the phosphorus-containing products from pyrolyzed BH to generate polyphosphate. The polyphosphate combined with AO from ATH and the expanded char layer from EG, forming a char layer with a better barrier effect. In ternary systems, ATH, BH, and EG can work together to generate an excellent condensed-phase synergistic flame retardant effect

Analysis of Delay-Throughput-Reliability Trade-off in Broadcasting Protocol for Vehicular Ad Hoc Networks

Broadcasting communications is critically important in vehicular ad hoc networks. Many safety applications need safety warning messages to be broadcast to all vehicles present in an area. Design of a broadcasting protocol for vehicular ad hoc networks is an interesting problem because of challenges posed by broadcasting traffic, high throughput, high reliability and low delay requirements of these networks. In this article, we present a mathematical analysis to explore the relationship between groups of parameters, namely contention windows, hidden/exposed vehicle nodes, reliability, throughput and delay of broadcasting protocol in vehicular ad hoc networks. Then, the multi-object optimization model is proposed to explore how to adjust the delay-throughput-reliability trade-off. The numerical analysis reveals that to keep balance between throughput, delay and reliability, the contention window of MAC may be dynamic adjust

Network Covert Channel Detection with Cluster based on Hierarchy and Density

AbstractIn order to solve the problem one detection algorithm can only detect one kind of network covert channel, The detection approach hierarchical and density based cluster was purposed. Because the coding scheme of the covert channel would cause many similar data occurred repeatedly, the detection algorithm cluster based on density can be used to detect several kinds of the covert channels. Moreover, the detection approach cluster based on hierarchy and density is able to tackle of detection a noisy channel. Several detection tests were conducted, the detection results show that the algorithm can work well to distinguish the covert channel from normal network traffic even the noise level was about 20%

Mulberry Leaf Polysaccharides Attenuate Oxidative Stress Injury in Peripheral Blood Leukocytes by Regulating Endoplasmic Reticulum Stress

The present study assessed the protective effects and underlying mechanisms of mulberry leaf polysaccharides (MLPs) against hydrogen peroxide (H2O2)-induced oxidative stress injury in the peripheral blood leukocytes (PBLs) of Megalobrama amblycephala. Five treatment groups were established in vitro: the NC group (PBLs incubated in an RPMI-1640 complete medium for 4 h), the HP group (PBLs incubated in an RPMI-1640 complete medium for 3 h, and then stimulated with 100 μM of H2O2 for 1 h), and the 50/100/200-MLP pre-treatment groups (PBLs were pre-treated with MLPs (50, 100, and 200 μg/mL) for 3 h, and then stimulated with 100 μM of H2O2 for 1 h). The results showed that MLP pre-treatment dose-dependently enhanced PBLs’ antioxidant capacities. The 200 μg/mL MLP pre-treatment effectively protected the antioxidant system of PBLs from H2O2-induced oxidative damage by reducing the malondialdehyde content and lactic dehydrogenase cytotoxicity, and increasing catalase and superoxide dismutase activities (p p foxO1α), binding immunoglobulin protein (bip), activating transcription factor 6 (atf6), and C/EBP-homologous protein (chop)), Ca2+ transport-related genes (voltage-dependent anion-selective channel 1 (vdac1), mitofusin 2 (mfn2), and mitochondrial Ca2+ uniporter (mcu)), and interleukin 6 (il-6) and bcl2-associated x (bax) were significantly lower in the 200-MLP pre-treatment group than in the HP group (p p > 0.05). These results indicated that MLP pre-treatment attenuated H2O2-induced PBL oxidative damage in the M. amblycephala by inhibiting endoplasmic reticulum stress and maintaining mitochondrial function. These findings also support the possibility that MLPs can be exploited as a natural dietary supplement for M. amblycephala, as they protect against oxidative damage

The Seismic Performance and Global Collapse Resistance Capacity of Infilled Reinforced Concrete Frames Considering the Axial–Shear–Bending Interaction of Columns

This paper presents a mechanism and method for simulating the axial–shear–bending interaction of a reinforced concrete (RC) column. The three-dimensional model of a multi-story infilled RC frame was modeled using the OpenSees software. Static pushover and nonlinear dynamic analyses under fortification and rare earthquakes were conducted using the model. Finally, based on the incremental dynamic analyses of 22 suites of ground-motion records, the global collapse resistance capacity of the infilled RC frame was evaluated using the evaluation method of a normal distribution. The analytical results show that the axial–shear–bending interaction is a key factor that affects the seismic response of infilled RC frames. Under the fortification earthquake condition, no obvious damage to physical structures was evident; the influence was relatively minor. However, under the condition of a rare earthquake, severe damage to physical structures was evident, resulting in the underestimation of the lateral inter-story drift ratio, while the degradation rates of the load capacity and global collapse resistance capacities for the infilled concrete frames were highly overestimated when the axial–shear–bending interaction was not considered

The Seismic Performance and Global Collapse Resistance Capacity of Infilled Reinforced Concrete Frames Considering the Axial–Shear–Bending Interaction of Columns

This paper presents a mechanism and method for simulating the axial–shear–bending interaction of a reinforced concrete (RC) column. The three-dimensional model of a multi-story infilled RC frame was modeled using the OpenSees software. Static pushover and nonlinear dynamic analyses under fortification and rare earthquakes were conducted using the model. Finally, based on the incremental dynamic analyses of 22 suites of ground-motion records, the global collapse resistance capacity of the infilled RC frame was evaluated using the evaluation method of a normal distribution. The analytical results show that the axial–shear–bending interaction is a key factor that affects the seismic response of infilled RC frames. Under the fortification earthquake condition, no obvious damage to physical structures was evident; the influence was relatively minor. However, under the condition of a rare earthquake, severe damage to physical structures was evident, resulting in the underestimation of the lateral inter-story drift ratio, while the degradation rates of the load capacity and global collapse resistance capacities for the infilled concrete frames were highly overestimated when the axial–shear–bending interaction was not considered