Multifocal laser direct writing through spatial light modulation guided by scalable vector graphics

Multifocal laser direct writing (LDW) based on phase-only spatial light modulator (SLM) can realize flexible and parallel nanofabrication with high throughput potential. In this investigation, a novel approach of combining two-photon absorption, SLM and vector path guided by scalable vector graphics (SVG) has been developed and tested preliminarily, for fast, flexible and parallel nanofabrication. Three laser focuses are independently controlled with different paths, which are according to SVG, to optimize fabrication and promote time efficiency. The minimum structure width can be as low as 74 nm. Accompanied with a translation stage, a carp structure of 18.16 $\mu$m by 24.35 $\mu$m has been fabricated. This method shows the possibility of developing LDW techniques towards full-electrical system, and provides a potential way to efficiently engrave complex structures on nanoscales

Glycyrrhizic acid alleviates the meconium-induced acute lung injury in neonatal rats by inhibiting oxidative stress through mediating the Keap1/Nrf2/HO-1 signal pathway

Meconium aspiration syndrome (MAS) is a disease closely related to inflammation and oxidative stress. Glycyrrhizic acid (GA) is a triterpenoid isolated from licorice with multiple bioprotective properties. In the present study, impacts of GA against MAS rats, as well as the potential mechanism, will be investigated. MAS model was established on newborn rats, followed by the treatment of 12.5, 25, and 50 mg/kg GA. The wet/dry weight ratio of lung tissues was calculated. The production of IL-6, IL-1β, TNF-α, malonaldehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) was measured using ELISA assay. HE staining was used to evaluate the pathological state of lung tissues and TUNEL assay was used to detect the apoptotic state. The protein expression of Nrf2, Keap1, HO-1, Bcl-2, Bax, and cleaved-Caspase3 was measured by Western blotting assay. The elevated W/D ratio, release of inflammatory factors, lung injury score, and apoptotic index, as well as the activated oxidative stress and suppressed Keap1/Nrf2/HO-1 pathway, in MAS rats were significantly alleviated by GA. After introducing the inhibitor of Nrf2, ML385, the protective property of GA on the pathological state, apoptotic index, and oxidative stress in MAS rats was pronouncedly abolished. Taken together, glycyrrhizin alleviated GAH in rats by suppressing Keap1/Nrf2/HO-1 signaling mediated oxidative stress

Progressively Hybrid Transformer for Multi-Modal Vehicle Re-Identification

Multi-modal (i.e., visible, near-infrared, and thermal-infrared) vehicle re-identification has good potential to search vehicles of interest in low illumination. However, due to the fact that different modalities have varying imaging characteristics, a proper multi-modal complementary information fusion is crucial to multi-modal vehicle re-identification. For that, this paper proposes a progressively hybrid transformer (PHT). The PHT method consists of two aspects: random hybrid augmentation (RHA) and a feature hybrid mechanism (FHM). Regarding RHA, an image random cropper and a local region hybrider are designed. The image random cropper simultaneously crops multi-modal images of random positions, random numbers, random sizes, and random aspect ratios to generate local regions. The local region hybrider fuses the cropped regions to let regions of each modal bring local structural characteristics of all modalities, mitigating modal differences at the beginning of feature learning. Regarding the FHM, a modal-specific controller and a modal information embedding are designed to effectively fuse multi-modal information at the feature level. Experimental results show the proposed method wins the state-of-the-art method by a larger 2.7% mAP on RGBNT100 and a larger 6.6% mAP on RGBN300, demonstrating that the proposed method can learn multi-modal complementary information effectively

Potential Therapeutic Effects of NAMPT-Mediated NAD Biosynthesis in Depression In Vivo

This study aimed to investigate the potential therapeutic effects of nicotinamide phosphoribosyltransferase (NAMPT)-mediated adenine dinucleotide (NAD) biosynthesis in depression models in vivo. Namptflox/flox mice were used to evaluate the role of NAMPT in depression. NAMPT and NAD levels in the prefrontal cortex (PFC) were measured, and depression-associated behavior, cognitive function, and social interaction were evaluated. The expression levels of BDNF, pCREB, CREB, monoamine neurotransmitters, and corticosterone (CORT) were also detected in the PFC. The contents of NAMPT and NAD decreased in the PFC in Namptflox/flox mice. Namptflox/flox mice showed depression-like behaviors, cognitive function deterioration, decreased social ability, and decreased dominance. Meanwhile, there were decreased expression levels of the pCREB/CREB ratio, but not BDNF, in the PFC. Levels of DA, 5-HT, and NE were decreased, and CORT was activated in the PFC of Namptflox/flox mice. Additionally, the role of NAMPT-NAD was examined in rats treated with nicotinamide riboside (NR) after being exposed to chronic unexpected mild stress (CUMS). NR reversed the decreased NAMPT expression in the PFC and HIP, and the NAD content in the PFC, but not HIP in rats with CUMS-induced depression. NR also improved depressive- and anxiolytic-like behaviors, locomotor activity, and cognitive function. BDNF expression and the pCREB/CREB ratio were significantly increased in both the PFC and HIP after NR treatment. The activation of CORT and decreased content of DA were reversed after NR treatment in the PFC. There was no difference in the 5-HT content among groups in both the PFC and HIP. Taken together, NAD synthesis induced by NAMPT could be associated with depression-like behaviors in mice, and the elevated NAD level by NR improved depression in rats

Discovery and characterizaton of a novel lipase with transesterification activity from hot spring metagenomic library

A new gene encoding a lipase (designated as Lip-1) was identified from a metagenomic bacterial artificial chromosome(BAC) library prepared from a concentrated water sample collected from a hot spring field in Niujie, Eryuan of Yunnan province in China. The open reading frame of this gene encoded 622 amino acid residues. It was cloned, fused with the oleosin gene and over expressed in Escherichia coli to prepare immobilized lipase artificial oil body AOB-sole-lip-1. The monomeric Sole-lip-1 fusion protein presented a molecular mass of 102.4 kDa. Enzyme assays using olive oil and methanol as the substrates in petroleum ether confirmed its transesterification activity. Hexadecanoic acid methyl ester, 8,11-Octadecadienoic acid methyl ester, 8-Octadecenoic acid methyl ester, and Octadecanoic acid methyl ester were detected. It showed favorable transesterification activity with optimal temperature 45 °C. Besides, the maximal biodiesel yield was obtained when the petroleum ether system as the organic solvent and the substrate methanol in 350 mmol/L (at a molar ratio of methanol of 10.5:1) and the water content was 1%. In light of these advantages, this lipase presents a promising resource for biodiesel production

Deep learning-driven precision control of dilution rate in multi-pass laser cladding: experiment and simulation

The continuous energy input can lead to heat accumulation in the multi-pass lap laser cladding, which results in a progressive increase in the dilution rate and deteriorates the quality of laser cladding. Precisely controlling the stability of the dilution in the multi-pass laser cladding is still challenging. In this study, we proposed a deep-learning driven method for precisely controlling the dilution rate in the multi-pass laser cladding. Initially, the relationship between the dilution rate and power energy is retracted via the experiment-based finite element simulation. Subsequently, the convolution neural network deep learning is applied to optimize and improve the accuracy of the dilution rates in the cladding layer. The experiment verifies that the high stability of dilution rate in each pass, i.e., average errors of less than 10.88%, is achieved via in-situ adjusting of the power energy using the prediction obtained from the proposed method. We also attempted to provide insights into the dilution mechanism in Invar alloy multi-pass laser cladding as well as the potential applications of this method for other materials and other additive manufacturing

Effect of misorientation of grain boundaries on the discontinuous precipitates of Cu–Ni–Si alloy

In this study, the effects of differences in grain size, grain boundary type and misorientation on the initiation of discontinuous precipitates are investigated for the Cu–5Ni–1.25Si alloy. Samples with different grain sizes are obtained by thermomechanical treatment, and the smaller the grain size, the higher the percentage of discontinuous precipitate at the same aging time, while there is no significant difference in the growth rate of the reaction front of discontinuous precipitates. EBSD is used to study the effect of grain boundary misorientation on discontinuous precipitates, the characteristics of discontinuous precipitates initiation on different grain boundaries are investigated by multivariate statistical analysis. It is found that discontinuous precipitates only initiate from random grain boundaries instead of coincident site lattice boundaries, where random grain boundaries with misorientation angles of 30–50° are more prone to discontinuous precipitates. And the initiation of DPs at different misorientation axes is different. There is a clear borderline for the axes such as , , and . The grain boundaries below this borderline do not initiate DP, while all grain boundaries below this angle initiate DPs. The cases for axes such as , , and are more complicated. On these misorientation axes, grain boundaries with lower or higher misorientation angles do not initiate DPs, while grain boundaries at intermediate range misorientation angles do initiate DPs

Spray-pyrolysis-assisted synthesis of yolk@shell anatase with rich oxygen vacancies for efficient sodium storage

Herein, yolk@shell structured anatase TiO2 microspheres are produced by a facile spray-pyrolysis-assisted method. Through introducing oxygen vacancies by hydrogen treatment, yolk@shell structured TiO2-x microspheres are also obtained. The as-synthesized yolk@shell TiO2-x demonstrates a high reversible capacity (230.7 mA h g(-1) after 200 cycles at 0.05 A g(-1)) and remarkable long-term cycling stability (capacity retention of 91.7% after 1000 cycles at 1 A g(-1)). DFT calculations reveal that introducing oxygen vacancies into TiO2 enhances the electrical conductivity, lowers the sodiation energy barrier, and facilitates Na+ diffusion kinetics. This work sheds light on the rational design of high-performance electrode materials through integrating the yolk@shell structure and oxygen vacancies

High-Performance Thin-Film Transistors with ZnO:H/ZnO Double Active Layers Fabricated at Room Temperature

H doping can enhance the performance of ZnO thin-film transistors (TFTs) to a certain extent, and the design of double active layers is an effective way to further improve a device’s performance. However, there are few studies on the combination of these two strategies. We fabricated TFTs with ZnO:H (4 nm)/ZnO (20 nm) double active layers by magnetron sputtering at room temperature, and studied the effect of the hydrogen flow ratio on the devices’ performance. ZnO:H/ZnO-TFT has the best overall performance when H2/(Ar + H2) = 0.13% with a mobility of 12.10 cm2/Vs, an on/off current ratio of 2.32 × 107, a subthreshold swing of 0.67 V/Dec, and a threshold voltage of 1.68 V, which is significantly better than the performance of single active layer ZnO:H-TFTs. This exhibits that the transport mechanism of carriers in double active layer devices is more complicated. On one hand, increasing the hydrogen flow ratio can more effectively suppress the oxygen-related defect states, thus reducing the carrier scattering and increasing the carrier concentration. On the other hand, the energy band analysis shows that electrons accumulate at the interface of the ZnO layer close to the ZnO:H layer, providing an additional path for carrier transport. Our research exhibits that the combination of a simple hydrogen doping process and double active layer construction can achieve the fabrication of high-performance ZnO-based TFTs, and that the whole room temperature process also provides important reference value for the subsequent development of flexible devices