Preparation and optimization of medicated cold cream using <i>Caralluma adscendens</i> var. <i>attenuata</i> for the treatment of <i>Candida</i> skin infection

Fungal skin infection is a major skin health issue worldwide. For the treatment of fungal infections, systematic antifungal therapies are frequently prescribed. The aim of this study is to prepare an antifungal cold cream from Caralluma adscendens var. attenuata to treat deep dermal fungal infection in the skin layer. To achieve this, different concentrations of plant extract-based cold cream were prepared, and their in vitro characteristic features such as color, texture, pH, viscosity, spreadability, stability, permeation, were analyzed together with ex vivo evaluation to identify their applicability in the treatment of acute rat skin irritation. After 72 h of induction of Candida albicans infection in rats (7 days, two times/day), C. adscendens var. attenuata cold cream was applied topically. In rats with C. albicans induction without any treatment, adverse skin damages were visible in the form of red rashes, whereas in those with the formulated cold cream application, significantly less skin damage and inflammation were observed on a dose-dependent basis. Moreover, the reduced microbial colonization and histopathology of the rat skin without any treatment indicated the successful invasion of C. albicans and showed the morphological changes caused by candidal infection. However, treatment with the C. adscendens var. attenuata cream significantly inhibited candida colonization and reversed the morphological changes. In addition, the formulated C. adscendens var. attenuata cold cream showed good spreadability, permeation, and viscosity. Hence, it can act as a potent antifungal topical agent for the treatment of C. albicans skin infection without any irritation, thus safeguarding the skin tissue

Preparation and optimization of medicated cold cream using Caralluma adscendens var. attenuata for the treatment of Candida skin infection

Fungal skin infection is a major skin health issue worldwide. For the treatment of fungal infections, systematic antifungal therapies are frequently prescribed. The aim of this study is to prepare an antifungal cold cream from Caralluma adscendens var. attenuata to treat deep dermal fungal infection in the skin layer. To achieve this, different concentrations of plant extract-based cold cream were prepared, and their in vitro characteristic features such as color, texture, pH, viscosity, spreadability, stability, permeation, were analyzed together with ex vivo evaluation to identify their applicability in the treatment of acute rat skin irritation. After 72 h of induction of Candida albicans infection in rats (7 days, two times/day), C. adscendens var. attenuata cold cream was applied topically. In rats with C. albicans induction without any treatment, adverse skin damages were visible in the form of red rashes, whereas in those with the formulated cold cream application, significantly less skin damage and inflammation were observed on a dose-dependent basis. Moreover, the reduced microbial colonization and histopathology of the rat skin without any treatment indicated the successful invasion of C. albicans and showed the morphological changes caused by candidal infection. However, treatment with the C. adscendens var. attenuata cream significantly inhibited candida colonization and reversed the morphological changes. In addition, the formulated C. adscendens var. attenuata cold cream showed good spreadability, permeation, and viscosity. Hence, it can act as a potent antifungal topical agent for the treatment of C. albicans skin infection without any irritation, thus safeguarding the skin tissue

PREPARATION OF COLD CREAM AGAINST CLINICAL PATHOGEN USING CARALLUMA ADSCENDENS VAR. ATTENUATA

Objective: A simple formulation of cold cream from methanolic extract Caralluma adscendens var. attenuata (MECA) and their antimicrobial activity was tested against various clinical pathogens, namely, Escherichia coli, methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecium, and Candida albicans. Methods: Methanol extract of these plant extract was prepared by the Soxhlet method. We analyzed phytochemical nature of theses plant, and subsequently, a cream was formulated cold-cream C. adscendens var. attenuata (FCA) different concentration such as FCA 50 mg, FCA 100 mg, and FCA 200 mg. In the present study, aimed to the antimicrobial activity of cold cream was measured by agar well diffusion method, and standard antibiotic Neosporin (market available) cream was used as positive control and dummy cold cream (without-MECA) were used as the negative control. Results: Phytochemical screening showed that the plant extracts were found a rich source of secondary metabolites. For more, the efficacy of cold cream from MECA extracts to against the clinical pathogen. Positive control Neosporin and 200 mg FCA cream was a highly significant difference in the zone of inhibition when compared to dummy cream. The 200 mg FCA was activity against Escherichia coli, methicillin-resistant Staphylococcus aureus, vancomycin-resistant E. faecium, and C. albicans highly significantly difference (p&lt;0.05) compared FCA 50 mg and FAC 100 mg creams. Conclusion: The results from this study suggested that the cold cream form base of MECA crude had antimicrobial activity in the different clinical pathogen. They could be used as an alternative source to conventional antimicrobial agents for the treatment of pathological infection

Characterization of Anatomical and Physiological Adaptations in Cassytha filiformis L.—An Advanced Obligate Hemiparasite on Morinda tinctoria Roxb.

A study was conducted to understand the host-parasite relationship in terms of anatomical and physiological adaptations in Morinda tinctoria Roxb. and Cassytha filiformis L. Anatomically the haustorium of Cassytha is found to have two parts, the upper haustorium and the endophyte. The former is the portion of a haustorium that lies external to the host organ, whereas the endophyte is the portion of a haustorium that penetrates host tissues. It was also observed that the host organ triggers the dedifferentiation of cortical parenchyma to develop dense cytoplasm, conspicuous nuclei and numerous starch grains and these cells are found to serve as the initials of upper haustorium. The level of Chl b was lower than Chl a and xanthophylls in Cassytha when compared to Morinda. The photosynthetic activity was measured in intact leaves/stems of both the host and parasitic plant using Chl a fluorescence induction kinetics, which revealed that the photosynthetic efficiency was very low in the infected sample as well as in the parasite stem. Over all, the reduction in the photosynthetic efficiency was correlated to the poorly developed PS II complex