La metodología de LEGO Juego Serio como herramienta pedagógica en escenarios educativos, empresariales y sociales.

La búsqueda de nuevos métodos de formación en los escenarios académicos empresariales y sociales a partir del uso de metodologías que involucren el desarrollo de habilidades cognitivas, estratégicas, desarrollo de la creatividad, el aprendizaje colaborativo y el trabajo en red, hace necesario el uso de herramientas pedagógicas que permita la construcción de un aprendizaje significativo, en donde los participantes sean parte activa de este proceso; es por ello que la aplicación de metodologías como LEGO Juego Serio permiten que el participante sea el eje central de este aprendizaje, cambiando el paradigma del profesor como eje central del proceso de aprendizaje

Incidencia del uso de la tecnología y la actividad física en estudiantes de 7 a 17 año

A cross-sectional descriptive study was conducted with 165 students of the District Institute of Educational Experiences in the city of Barranquilla, to assess the level of physical activity. Age, gender, educational level, socioeconomic status, BMI, waist circumference and physical activity using the Physical Activity Questionnaire (IPAQ-C) was evaluated. 64.9% of participants have 8-13 years, 51% male and 49% female, 21.2% belong to grade 5, 88.4% are of socioeconomic 3 and 4, 38.7% are overweight and obesity; and the average rate waist 67. In relation to sedentary comes in a 65.1%; one in five students are obese. It is recommended to promote physical education program with minimum of sixty minutes three times a week.Se realizó un estudio descriptivo transversal con 165 estudiantes del instituto Distrital de Experiencias Pedagógicas de la ciudad de Barranquilla, para valorar el nivel de actividad física. Se evaluó la edad, el género, el nivel académico, estrato socioeconómico, el IMC, perímetro de cintura y la actividad física con el uso del Cuestionario de actividad física (IPAQ-C). El 64.9% de los participantes tiene entre 8-13 años, el 51% son hombres y el 49% son mujeres, el 21.2% pertenecen al grado 5°, el 88.4% son de estrato socioeconómico 3 y 4, el 38.7% presentan sobrepeso y obesidad; y el promedio del índice de cintura de 67. Con relación a la actividad física el sedentarismo se presenta en un 65.1%; uno de cada cinco estudiantes son obesos. El 98% de los estudiantes dedican en promedio seis horas diarias a las actividades tecnológicas, así: videojuegos con un 48%, televisión con un 30% y uso de la computadora en un 20%. Sólo el 2% no realiza ningún tipo de actividad con el uso de la tecnología. Se recomienda promover un programa de educación física con mínimo de sesenta minutos, tres veces a la semana

La imagen y la narrativa como herramientas para el abordaje psicosocial en escenarios de violencia Departamento Bogotá Cundinamarca.

Con el fin de realizar un proceso reflexivo, contextualizado y reconociendo los diferentes relatos de víctimas y sobrevivientes dentro de las realidades individuales y sociales, por medio de la narrativa, en los múltiples entornos y situaciones de violencia armada, social y política, de tal forma que se logre desarrollar el rol de guía y liderazgo tanto en técnicas como en recursos de afrontamiento de la realidad que permitan a las víctimas y sobrevivientes realizar procesos de resiliencia y empoderamiento en proyectos de vida productivos; abordamos un caso real para realizar ampliación de la reflexión por medio de un proceso argumentativo. Posteriormente se procede a plantear 3 tipos de preguntas; estratégicas, circulares y reflexivas, las cuales apoyan en los procesos de acercamiento psicosocial y desarrollo de estrategias de abordaje con objetivos específicos definidos, luego se presenta un análisis y planteamiento de estrategias de abordaje psicosocial basadas en un caso de estudio representativo del conflicto armado colombiano, con el fin de describir las realidades, reflexionar sobre los hechos narrados, realizar propuestas sobre la intervención y estrategias de acompañamiento psicosocial a desarrollar. De la misma forma presentamos el informe analítico y reflexivo de la experiencia realizada en la actividad de foto voz en donde cada integrante del grupo de trabajo en el contexto escogido realizo un proceso de reconocimiento de violencia basado en realidades de contextos locales, urbanos, rurales y finalmente se presentará el registro fotográfico y resultado del ejercicio narrativo visual como apoyo al proceso subjetivo de metaforizacion.In order to carry out a reflexive, contextualized process and recognizing the different stories of victims and survivors within the individual and social realities, through the narrative, in the multiple environments and situations of armed, social and political violence, in such a way so that we can empower ourselves within the processes in the role of guidance and leadership in coping techniques and resources, and identification of the reality, that allow victims and survivors, to carry out processes of resilience and empowerment in productive life projects, we approach a real case to broaden the reflection through an argumentative process. Subsequently proceed to raise 3 types of questions; strategic, circular and reflective, which support the processes of psychosocial approach and development of strategies of approach with specific defined objectives, then an analysis and approach of psychosocial approach strategies based on a case study representative of the Colombian armed conflict is presented, In order to describe the realities, reflect on the narrated facts, make proposals about the intervention and psychosocial accompaniment strategies to be developed. In the same way we present the analytical and reflective report of the experience carried out in the photo voice process where each member of the working group in the chosen context carried out a recognition process of violence based on realities of local, urban, rural and local contexts. finally, the photographic record and result of the visual narrative exercise will be presented as support to the subjective process of metaphorizatio

Mendelian Randomization Analysis of the Relationship Between Native American Ancestry and Gallbladder Cancer Risk

Background A strong association between the proportion of Native American ancestry and the risk of gallbladder cancer (GBC) has been reported in observational studies. Chileans show the highest incidence of GBC worldwide, and the Mapuche are the largest Native American people in Chile. We set out to investigate the causal association between Native American Mapuche ancestry and GBC risk, and the possible mediating effects of gallstone disease and body mass index (BMI) on this association. Methods Markers of Mapuche ancestry were selected based on the informativeness for assignment measure and then used as instrumental variables in two-sample mendelian randomization (MR) analyses and complementary sensitivity analyses. Result We found evidence of a causal effect of Mapuche ancestry on GBC risk (inverse variance-weighted (IVW) risk increase of 0.8% for every 1% increase in Mapuche ancestry proportion, 95% CI 0.4% to 1.2%, p = 6.6×10-5). Mapuche ancestry was also causally linked to gallstone disease (IVW risk increase of 3.6% per 1% increase in Mapuche proportion, 95% CI 3.1% to 4.0%, p = 1.0×10-59), suggesting a mediating effect of gallstones in the relationship between Mapuche ancestry and GBC. In contrast, the proportion of Mapuche ancestry showed a negative causal effect on BMI (IVW estimate -0.006 kg/m2 per 1% increase in Mapuche proportion, 95% CI -0.009 to -0.003, p = 4.4×10-5). Conclusions The results presented here may have significant implications for GBC prevention and are important for future admixture mapping studies. Given that the association between Mapuche ancestry and GBC risk previously noted in observational studies appears to be causal, primary and secondary prevention strategies that take into account the individual proportion of Mapuche ancestry could be particularly efficient

Immunoglobulin, glucocorticoid, or combination therapy for multisystem inflammatory syndrome in children: a propensity-weighted cohort study.

BACKGROUND: Multisystem inflammatory syndrome in children (MIS-C), a hyperinflammatory condition associated with SARS-CoV-2 infection, has emerged as a serious illness in children worldwide. Immunoglobulin or glucocorticoids, or both, are currently recommended treatments. METHODS: The Best Available Treatment Study evaluated immunomodulatory treatments for MIS-C in an international observational cohort. Analysis of the first 614 patients was previously reported. In this propensity-weighted cohort study, clinical and outcome data from children with suspected or proven MIS-C were collected onto a web-based Research Electronic Data Capture database. After excluding neonates and incomplete or duplicate records, inverse probability weighting was used to compare primary treatments with intravenous immunoglobulin, intravenous immunoglobulin plus glucocorticoids, or glucocorticoids alone, using intravenous immunoglobulin as the reference treatment. Primary outcomes were a composite of inotropic or ventilator support from the second day after treatment initiation, or death, and time to improvement on an ordinal clinical severity scale. Secondary outcomes included treatment escalation, clinical deterioration, fever, and coronary artery aneurysm occurrence and resolution. This study is registered with the ISRCTN registry, ISRCTN69546370. FINDINGS: We enrolled 2101 children (aged 0 months to 19 years) with clinically diagnosed MIS-C from 39 countries between June 14, 2020, and April 25, 2022, and, following exclusions, 2009 patients were included for analysis (median age 8·0 years [IQR 4·2-11·4], 1191 [59·3%] male and 818 [40·7%] female, and 825 [41·1%] White). 680 (33·8%) patients received primary treatment with intravenous immunoglobulin, 698 (34·7%) with intravenous immunoglobulin plus glucocorticoids, 487 (24·2%) with glucocorticoids alone; 59 (2·9%) patients received other combinations, including biologicals, and 85 (4·2%) patients received no immunomodulators. There were no significant differences between treatments for primary outcomes for the 1586 patients with complete baseline and outcome data that were considered for primary analysis. Adjusted odds ratios for ventilation, inotropic support, or death were 1·09 (95% CI 0·75-1·58; corrected p value=1·00) for intravenous immunoglobulin plus glucocorticoids and 0·93 (0·58-1·47; corrected p value=1·00) for glucocorticoids alone, versus intravenous immunoglobulin alone. Adjusted average hazard ratios for time to improvement were 1·04 (95% CI 0·91-1·20; corrected p value=1·00) for intravenous immunoglobulin plus glucocorticoids, and 0·84 (0·70-1·00; corrected p value=0·22) for glucocorticoids alone, versus intravenous immunoglobulin alone. Treatment escalation was less frequent for intravenous immunoglobulin plus glucocorticoids (OR 0·15 [95% CI 0·11-0·20]; p<0·0001) and glucocorticoids alone (0·68 [0·50-0·93]; p=0·014) versus intravenous immunoglobulin alone. Persistent fever (from day 2 onward) was less common with intravenous immunoglobulin plus glucocorticoids compared with either intravenous immunoglobulin alone (OR 0·50 [95% CI 0·38-0·67]; p<0·0001) or glucocorticoids alone (0·63 [0·45-0·88]; p=0·0058). Coronary artery aneurysm occurrence and resolution did not differ significantly between treatment groups. INTERPRETATION: Recovery rates, including occurrence and resolution of coronary artery aneurysms, were similar for primary treatment with intravenous immunoglobulin when compared to glucocorticoids or intravenous immunoglobulin plus glucocorticoids. Initial treatment with glucocorticoids appears to be a safe alternative to immunoglobulin or combined therapy, and might be advantageous in view of the cost and limited availability of intravenous immunoglobulin in many countries. FUNDING: Imperial College London, the European Union's Horizon 2020, Wellcome Trust, the Medical Research Foundation, UK National Institute for Health and Care Research, and National Institutes of Health

Caracterización del pensamiento narrativo en preescolares

El presente estudio corresponde a un diseño mixto que incorpora elementos cualitativos y cuantitativos, de tipo descriptivo. Este documento presenta la caracterización del pensamiento narrativo de niños preescolares a partir del enfoque histórico cultural de Vigotsky, la teoría de la actividad de Talizaina y la propuesta de pensamiento narrativo de colombianas. A los niños que participaron en este estudio se les aplicó un programa de intervención cuyo eje principal era la actividad de cuento. El análisis de los resultados evidencio que el pensamiento narrativo de los niños se caracteriza por la identificación de los elementos constituyentes de la narrativa: inicio. Desarrollo y sentido final, el uso de expresiones lingüísticas variadas en donde predominan verbos y estados emocionales, así como establecimiento de relaciones entre con sus experiencias familiares y sociales

Insulin and TOR signal in parallel through FOXO and S6K to promote epithelial wound healing

The TOR and Insulin/IGF signalling (IIS) network controls growth, metabolism and ageing. Although reducing TOR or insulin signalling can be beneficial for ageing, it can be detrimental for wound healing, but the reasons for this difference are unknown. Here we show that IIS is activated in the cells surrounding an epidermal wound in Drosophila melanogaster larvae, resulting in PI3K activation and redistribution of the transcription factor FOXO. Insulin and TOR signalling are independently necessary for normal wound healing, with FOXO and S6K as their respective effectors. IIS is specifically required in cells surrounding the wound, and the effect is independent of glycogen metabolism. Insulin signalling is needed for the efficient assembly of an actomyosin cable around the wound, and constitutively active myosin II regulatory light chain suppresses the effects of reduced IIS. These findings may have implications for the role of insulin signalling and FOXO activation in diabetic wound healing

MicroRNA-190b Targets RFWD3 in Estrogen Receptor–Positive Breast Cancer

Background: In the year 2020, breast cancer was the most common form of cancer worldwide. Roughly 70% of breast cancers are estrogen receptor–positive (ER+). MicroRNA-190b (miR-190b) has previously been reported to be upregulated in ER+ breast cancers. Previously, we have demonstrated that miR-190b is hypomethylated in ER+ breast cancers, potentially leading to its upregulation. Objectives: To further study the role of miR-190b in ER+ breast cancer and to identify its clinically relevant targets in breast cancer. Design: Patient cohort and cell line–based RNA-sequencing analysis. Methods: The Cancer Genome Atlas was used to obtain gene expression data and clinical information on patients with breast cancer. To identify messenger RNA (mRNA) targets for miR-190b, the ER+ breast cancer cell line T-47D was used to immunoprecipitate biotin-labeled miR-190b followed by RNA sequencing. Western blot was used to confirm miR-190b target. Patient survival based on miR-190b and selected target was studied using the Cancer Genome Atlas. Results: In this study, we confirm that miR-190b is overexpressed in breast cancer via differential expression analysis and show that high expression of miR-190b results in more favorable outcomes in Luminal A patients, hazard ratio (HR) = 0.29, 95% confidence interval [CI] = 0.12-0.71, P  = .0063. MicroRNA-190b target analysis identified RING finger and WD repeat domain 3 (RFWD3) as one of miR-190b regulatory targets in ER+ breast cancer. Survival analysis of RFWD3 showed that elevated levels result in poorer overall survival in patients with Luminal A breast cancer (HR = 2.22, 95% CI = 1.33-3.71, P  = .002). Gene ontology analysis of our sequencing results indicates that miR-190b may have a role in breast cancer development and/or tumorigenesis and that it may be a suitable tool in characterization between the ER+ subtypes, Luminal A, and Luminal B. Conclusions: We show that miR-190b targets RFWD3 in ER+ breast cancers leading to lower RFWD3 protein expression. Low levels of RFWD3 are associated with better outcomes in patients with Luminal A breast cancer but not in patients with Luminal B breast cancer. These findings provide novel insights into miR-190b role in breast cancer and that its clinical relevance is subtype specific

Determinación de manejos más frecuentes en ira no neumónica en menores de cinco años. Hospital Nazareth, Barranquilla,2012

Background: Acute respiratory infections are the leading cause of pediatric outpatient and emergency, which on average constitute 60% of consultations, being considered for several decades as a public health problem, being responsible for the deaths of about 4 million children a year, mostly in developing countries of Asia, Africa and Latin America. Objective: Determine the most common management of acute respiratory infections (non-pneumonic) in chil- dren from the age of two months to five years old. Methodology: We designed a cross-sectional descriptive study conducted at the Nazareth Hospital in Barranquilla (Colombia). Sample of 50 patients, primary source information obtained by surveys, and secondary through medi- cal records. Results: We found that the most frequently used maneuvers were related to non-pharmacological measures for physicians and caregivers. For the common cold, pharmacologic and nonpharmacologic treatment met equal per- centage; the pharmacological prevailed in otitis media, and non-pharmacological measures in addressing croup for both health professionals to caregivers, but the conservative management was done only by professionals traf- fickers (inhalers, intravenous fluids, epinephrine and oxygen). Excels the high percentage of cases where specific drugs are used, among other, as antibiotics, by 60% for sore throat physicians, and in the croup 40 % were treated with antibiotics for caregivers, demonstrating an inappropriate use of these drugs.x- terna y urgencia, donde en promedio constituyen el 60% de las consultas, siendo consideradas desde hace va décadas como un problema de salud pública, responsables de la muerte de alrededor de 4 millones de niños por año, principalmente en países en vías de desarrollo de Asia, África y América Latina. Objetivo: Determinar el manejo más frecuente de las infecciones respiratorias agudas (no neumónicas) en niños de dos meses a cinco años de edad. Metodología: Se diseñó un estudio descriptivo de corte transversal realizado en el Hospital Nazareth de Barran- quilla (Colombia). Muestra de 50 pacientes, información obtenida mediante fuente primaria por encuestas, y secundaria a través de historias clínicas. Resultados: Se encontró que los manejos utilizados con mayor frecuencia fueron los relacionados con medidas no farmacológicas por médicos tratantes y acudientes. Para el resfriado común, el tratamiento farmacológico y no farmacológico se encontró con igualdad de porcentaje, el farmacológico primó en otitis media, y medidas no farmacológicas en el abordaje del crup tanto para profesionales de la salud como para acudientes, aunque aquí se observó un manejo farmacológico que solo fue realizado por profesionales tratantes (inhaladores, hidratación parenteral, adrenalina y oxígeno). Sobresale el elevado porcentaje de casos donde se utilizan medicamentos es- pecíficos, entre otros, como antibióticos, en un 60% para faringoamigdalitis por médicos tratantes, y para el caso UP el 40% fueron tratados con antibióticos por acudientes, demostrando un uso inapropiado de estos fármacos