Effects of taurine on male reproduction in rats of different ages

<p>Abstract</p> <p>Background</p> <p>It has been demonstrated that taurine is one of the most abundant free amino acids in the male reproductive system, and can be biosynthesized by male reproductive organs. But the effect of taurine on male reproduction is poorly understood.</p> <p>Methods</p> <p>Taurine and β-alanine (taurine transport inhibitor) were offered in water to male rats of different ages. The effects of taurine on reproductive hormones, testis marker enzymes, antioxidative ability and sperm quality were investigated.</p> <p>Results</p> <p>The levels of T and LH were obviously increased by taurine supplementation in rats of different ages, and the level of E was also significantly elevated in baby rats. The levels of SOD, ACP, SDH and NOS were obviously increased by taurine administration in adult rats, but the levels of AKP, AST, ALT and NO were significantly decreased. The levels of SOD, ACP, LDH, SDH, NOS, NO and GSH were significantly elevated by taurine administration in aged rats, but the levels of AST and ALT were significantly decreased. The motility of spermatozoa was obviously increased by taurine supplement in adult rats. The numbers and motility of spermatozoa, the rate of live spermatozoa were significantly increased by taurine supplement in aged rats.</p> <p>Conclusions</p> <p>The present study demonstrated that a taurine supplement could stimulate the secretion of LH and T, increase the levels of testicular marker enzymes, elevate testicular antioxidation and improve sperm quality. The results imply that taurine plays important roles in male reproduction especially in aged animals.</p

Fan and Ventilation Rate Monitoring of Cage-Free Layer Houses in California

Ventilation rates were continuously monitored in two cage-free layer houses located in California from March 1, 2012 to May 13, 2013. The average number of brown Lohmann laying hens in each house was 33,300. Temperature, relative humidity, static pressure, and running status of 48 ventilation fans were continuously monitored and recorded every minute. Regression models were developed to relate house temperature and ventilation rate to inlet air temperature, and to relate airflow rate to building static pressure (R2 = 0.98). Results showed that the daily mean ventilation rate per hen ranged from 1.91 to 8.72 m3 h-1 hen-1, averaging at 4.49 ±1.53 m3 h-1 hen-1. The standard uncertainty of daily mean ventilation rate was determined to be 3.7%. The 91-cm and 130-cm fans were found to perform at 82% and 63% of the manufacturer rated airflow rates, respectively. The minimum and maximum static pressure was 11.0 and 50.6 Pa, respectively, corresponding to 2 and 16 running tunnel fans. When the house temperature exceeded 30ºC, an evaporative cooling system was activated, which could reduce the inlet air temperature by 6.3ºC and concurrently increased the indoor air humidity ratio by 3.4 g per kg dry air. Cooling pad efficiency was 66%. The sidewall fans and tunnel fans were operated at 65% and 20% of the total time when layers occupied the houses. The new rational formula to calculate dry base ventilation rates was developed based upon the ratio of water vapor volume to moist air volume. The developed models and data collected in this research can be used to calculate the ventilation rates in cage-free layer houses so that it can be possible to assure healthy conditions needed for layers. They can also be used in the design of cage free houses and in the calculations of emissions of air pollutants from these houses

Stigmatized Stroke? A Qualitative Study of Perception of Stroke Among Community Residents With Hypertension

Objectives: To understand the perception of stroke in the hypertensive population. Hypertension is the primary risk factor for stroke, and current approaches to stroke prevention are inadequate and often fragmented. Understanding the perception of stroke among individuals with hypertension is crucial for a targeted approach. However, empirical evidence on this perception is limited.Methods: A qualitative design involved thematic analysis of focus groups and interview data from urban China with hypertension. Audio recordings were transcribed and subjected to thematic analysis.Results: Three themes were identified. Hypertensive participants first identified stroke patients by their obvious physical disability, and then identified the disease as a negative thing. Finally, they wanted to stay away from stroke, but paradoxically, there is a contradictory approach to avoidance and prevention, such as being willing to prevent the disease or simply avoiding socializing with stroke patients.Conclusion: Hypertensive patients hold complex and diverse perceptions of stroke, including a certain stigma. Future public health education should prioritize improving media promotion and fostering interaction between patients with hypertension and stroke in the community

Metagenomic Next-Generation Sequencing in the Diagnosis of HHV-1 Reactivation in a Critically Ill COVID-19 Patient: A Case Report

Background: Secondary infections pose tremendous challenges in Coronavirus disease 2019 (COVID-19) treatment and are associated with higher mortality rates. Clinicians face of the challenge of diagnosing viral infections because of low sensitivity of available laboratory tests. Case Presentation: A 66-year-old woman initially manifested fever and shortness of breath. She was diagnosed as critically ill with COVID-19 using quantitative reverse transcription PCR (RT-qPCR) and treated with antiviral therapy, ventilator and extracorporeal membrane oxygenation (ECMO). However, after the condition was relatively stabled for a few days, the patient deteriorated with fever, frequent cough, increased airway secretions, and increased exudative lesions in the lower right lung on chest X-rays, showing the possibility of a newly acquired infection, though sputum bacterial and fungal cultures and smears showed negative results. Using metagenomic next-generation sequencing (mNGS), we identified a reactivation of latent human herpes virus type 1 (HHV-1) in the respiratory tract, blood and gastrointestinal tract, resulting in a worsened clinical course in a critically ill COVID-19 patient on ECMO. Anti-HHV-1 therapy guided by these sequencing results effectively decreased HHV-1 levels, and improved the patient\u27s clinical condition. After 49 days on ECMO and 67 days on the ventilator, the 66-year-old patient recovered and was discharged. Conclusions: This case report demonstrates the potential value of mNGS for evidence-based treatment, and suggests that potential reactivation of latent viruses should be considered in critically ill COVID-19 patients

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data