1 research outputs found
Influence of three different histological methods on the morphology and morphometrical data in human testis
Coagulant fixatives and paraffin embedding
were widely used in the past for histomorphometrical
evaluations of the human testis under physiological and
pathological conditions. However, new methods are
applied nowadays using better combinations of fixatives
and plastic resins as embedding media, improving cell
and tissue structural preservation. In an attempt to
compare old and new data, the present study evaluated
histomorphometrical data obtained from human testis
after three different histological processing methods:
Bouin/paraplast, glutaraldehyde/glycol methacrylate and
glutaraldehyde/araldite. The morphometrical parameters
were not affected by glutaraldehyde fixation after both
resin embedding (methacrylate or araldite). On the other
hand, Bouin/paraplast embedding lead to tissue
shrinkage, which could give rise to misinterpretations on
the measurements performed. Since some germ and
somatic cells recognition do not depend upon high
resolution techniques, counting of such cell types could
be performed even using routine Bouin/paraplast
protocols. Thus, the morphometrical analyses relying on
cell recognition were not affected by the methods here
applied, however, when metric measurements were
applied, the obtained results could not be promptly
compared. On the other hand, if the study requires
confident spermatogonial identification for kinetics
evaluation, glutaraldehyde/araldite processing is highly
recommended