Validating the use of the shuttle walking test in healthy adult women

The Shuttle Walking Test (SWT), with its externally paced characteristics, is commonly used as an objective measure of functional capacity. The reliability and validity of the SWT has been previously shown but only in patient populations. No studies have been carried out to investigate the validity of the SWT in healthy adult women. Therefore, the primary aim of this test was to determine if the SWT is a valid field measure of cardiorespiratory fitness in healthy adult women. A secondary aim was to identify if variables, such as age, body composition and habitual physical activity influence performance on the SWT. The distance ambulated on the SWT was compared with a standard laboratory test of cardiorespiratory capacity, peak oxygen consumption ( VO2peak) determined on an Individualised Balke Treadmill Test (IBT). Thirty-four healthy adult women with an age range of 32-65 yrs completed both exercise tests. Mean (± SD) SWT distance 624.5 (148.9) m and VO2peak 29.4 (7.8) ml.kg-1.min-1 were higher than that shown in previous studies of patient populations. Pearson product moment correlation analysis indicated a moderate but significant relationship (r = 0.58, p = 0.0005) between SWT distance and VO2peak. Variability in performance on the SWT can be explained partly by age and estimated body fat. This study is the first to investigate the validity of the SWT in healthy adult women. The correlation with VO2peak from IBT was lower than that in previous studies with patient populations. The findings suggest that performance on the SWT in healthy adult women is limited by locor.10tor ability as well as cardiorespiratory fitness. Therefore, the use of SWT as a field measure of cardiorespiratory fitness in healthy adult women has limitations. The study provides the basis for further work to modify the SWT for use in a healthy adult population

Mesothelial cells in tissue repair and fibrosis

Mesothelial cells are fundamental to the maintenance of serosal integrity and homeostasis and play a critical role in normal serosal repair following injury. However, when normal repair mechanisms breakdown, mesothelial cells take on a profibrotic role, secreting inflammatory, and profibrotic mediators, differentiating and migrating into the injured tissues where they contribute to fibrogenesis. The development of new molecular and cell tracking techniques has made it possible to examine the origin of fibrotic cells within damaged tissues and to elucidate the roles they play in inflammation and fibrosis. In addition to secreting proinflammatory mediators and contributing to both coagulation and fibrinolysis, mesothelial cells undergo mesothelial-to-mesenchymal transition, a process analogous to epithelial-to-mesenchymal transition, and become fibrogenic cells. Fibrogenic mesothelial cells have now been identified in tissues where they have not previously been thought to occur, such as within the parenchyma of the fibrotic lung. These findings show a direct role for mesothelial cells in fibrogenesis and open therapeutic strategies to prevent or reverse the fibrotic process

Ginseng and Ginkgo Biloba effects on cognition as modulated by cardiovascular reactivity: A randomised trial

Background There is some evidence to suggest that ginseng and Ginkgo biloba can improve cognitive performance, however, very little is known about the mechanisms associated with such improvement. Here, we tested whether cardiovascular reactivity to a task is associated with cognitive improvement. Methodology/Principal findings Using a double-blind, placebo controlled, crossover design, participants (N = 24) received two doses of Panax Ginseng (500, 1000 mg) or Ginkgo Biloba (120, 240 mg) (N = 24), and underwent a series of cognitive tests while systolic, diastolic, and heart rate readings were taken. Ginkgo Biloba improved aspects of executive functioning (Stroop and Berg tasks) in females but not in males. Ginseng had no effect on cognition. Ginkgo biloba in females reversed the initial (i.e. placebo) increase in cardiovascular reactivity (systolic and diastolic readings increased compared to baseline) to cognitive tasks. This effect (reversal) was most notable after those tasks (Stroop and Iowa) that elicited the greatest cardiovascular reactivity during placebo. In males, although ginkgo also decreased cardiovascular readings, it did so from an initial (placebo) blunted response (i.e. decrease or no change from baseline) to cognitive tasks. Ginseng, on the contrary, increased cardiovascular readings compared to placebo. Conclusions/Significance These results suggest that cardiovascular reactivity may be a mechanism by which ginkgo but not ginseng, in females is associated with certain forms of cognitive improvement

Mitochondria-derived reactive oxygen species drive GANT61-induced mesothelioma cell apoptosis

Gli transcription factors of the Hedgehog (Hh) pathway have been reported to be drivers of malignant mesothelioma (MMe) cell survival. The Gli inhibitor GANT61 induces apoptosis in various cancer cell models, and has been associated directly with Gli inhibition. However various chemotherapeutics can induce cell death through generation of reactive oxygen species (ROS) but whether ROS mediates GANT61-induced apoptosis is unknown. In this study human MMe cells were treated with GANT61 and the mechanisms regulating cell death investigated. Exposure of MMe cells to GANT61 led to G1 phase arrest and apoptosis, which involved ROS but not its purported targets, GLI1 or GLI2. GANT61 triggered ROS generation and quenching of ROS protected MMe cells from GANT61-induced apoptosis. Furthermore, we demonstrated that mitochondria are important in mediating GANT61 effects: (1) ROS production and apoptosis were blocked by mitochondrial inhibitor rotenone; (2) GANT61 promoted superoxide formation in mitochondria; and (3) mitochondrial DNA-deficient LO68 cells failed to induce superoxide, and were more resistant to apoptosis induced by GANT61 than wild-type cells. Our data demonstrate for the first time that GANT61 induces apoptosis by promoting mitochondrial superoxide generation independent of Gli inhibition, and highlights the therapeutic potential of mitochondrial ROS-mediated anticancer drugs in MMe

Sulphonylurea Usage in Melioidosis Is Associated with Severe Disease and Suppressed Immune Response

10.1371/journal.pntd.0002795PLoS Neglected Tropical Diseases84

Can patterns of everyday consumption indicate lifestyles? A secondary analysis of expenditures for fast moving goods and their social contexts

Ausgangspunkt der vorliegenden Studie bildet die Frage, ob sich Einkaufsformen beim täglichen Verbrauch als grundlegende Indikatoren von Lebensstilen erweisen. Datengrundlage bildet eine Sekundäranalyse von Panel-Daten zum Konsum schnelllebiger Waren, die vom weltweiten Marktforschungsunternehmen GfK im Jahr 1995 erhoben worden sind. Es wird zunächst eine Reihe von Hypothesen aufgestellt und die zugrunde gelegten Daten und die Stichprobe erläutert. Im Hauptteil der Studie werden 15 vollständige Cluster des Kaufverhaltens von Waren aus den Bereichen Nahrungsmittel, Getränke und Hygiene-Artikel untersucht und mit ausgewählten sozialen Indikatoren, wie z.B. sozialer Status, Mentalität und Umweltbewusstsein in Beziehung gesetzt. Die Ergebnisse zeigen, dass selbst der Kauf von schnelllebigen Konsumgütern in breitere Lebensstile eingebettet ist und auch im Zeitverlauf relativ konstant bleibt. (ICI

Mutational Analysis of Hedgehog Signaling Pathway Genes in Human Malignant Mesothelioma

Background The Hedgehog (HH) signaling pathway is critical for embryonic development and adult homeostasis. Recent studies have identified regulatory roles for this pathway in certain cancers with mutations in the HH pathway genes. The extent to which mutations of the HH pathway genes are involved in the pathogenesis of malignant mesothelioma (MMe) is unknown. Methodology/Principal Findings Real-time PCR analysis of HH pathway genes PTCH1, GLI1 and GLI2 were performed on 7 human MMe cell lines. Exon sequencing of 13 HH pathway genes was also performed in cell lines and human MMe tumors. In silico programs were used to predict the likelihood that an amino-acid substitution would have a functional effect. GLI1, GLI2 and PTCH1 were highly expressed in MMe cells, indicative of active HH signaling. PTCH1, SMO and SUFU mutations were found in 2 of 11 MMe cell lines examined. A non-synonymous missense SUFU mutation (p.T411M) was identified in LO68 cells. In silico characterization of the SUFU mutant suggested that the p.T411M mutation might alter protein function. However, we were unable to demonstrate any functional effect of this mutation on Gli activity. Deletion of exons of the PTCH1 gene was found in JU77 cells, resulting in loss of one of two extracellular loops implicated in HH ligand binding and the intracellular C-terminal domain. A 3-bp insertion (69_70insCTG) in SMO, predicting an additional leucine residue in the signal peptide segment of SMO protein was also identified in LO68 cells and a MMe tumour. Conclusions/Significance We identified the first novel mutations in PTCH1, SUFU and SMO associated with MMe. Although HH pathway mutations are relatively rare in MMe, these data suggest a possible role for dysfunctional HH pathway in the pathogenesis of a subgroup of MMe and help rationalize the exploration of HH pathway inhibitors for MMe therapy

Oseltamivir- and Amantadine-Resistant Influenza Viruses A (H1N1)

Surveillance of amantadine and oseltamivir resistance among influenza viruses was begun in Hong Kong in 2006. In 2008, while both A/Brisbane/59/2007-like and A/Hong Kong/2652/2006-like viruses (H1N1) were cocirculating, we detected amantadine and oseltamivir resistance among A/Hong Kong/2652/2006-like viruses (H1N1), caused by genetic reassortment or spontaneous mutation