Coronary artery bypass grafting in patients with left main coronary artery stenosis. Improved survival in a predominantly Oriental population

Journal of Cardiovascular Surgery334464-467JCVS

Surgery for left main spasm. Is it indicated?

10.1016/0167-5273(96)02604-6International Journal of Cardiology543213-216IJCD

NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA

10.1088/0957-4484/20/15/155101Nanotechnology2015-NNOT

Therapeutic angiogenesis for coronary artery disease

Journal of Cardiac Surgery174350-354JCAS

Hybrid cardiac revascularisation surgery

Singapore Medical Journal41136-38SIMJ

Co-Existing Left Atrial Thrombus and Myxoma in Mitral Stenosis - A Diagnostic Challenge

Singapore Medical Journal40146-47SIMJ

Coronary artery bypass surgery in young patients

Australian and New Zealand Journal of Surgery628618-621ANZJ

Small hiatal hernia and postprandial reflux after vertical sleeve gastrectomy: A multiethnic Asian cohort

10.1371/journal.pone.0241847PLoS ONE1511-Nove024184

Comparison of human and porcine aortic valves

10.1002/ca.10149Clinical Anatomy163193-196CLAN

Derivation of clinically compliant MSCs from CD105+, CD24-differentiated human ESCs

Adult tissue-derived mesenchymal stem cells (MSCs) have demonstrated therapeutic efficacy in treating diseases or repairing damaged tissues through mechanisms thought to be mediated by either cell replacement or secretion of paracrine factors. Characterized, self-renewing human ESCs could potentially be an invariable source of consistently uniform MSCs for therapeutic applications. Here we describe a clinically relevant and reproducible manner of generating identical batches of hESC-derived MSC (hESC-MSC) cultures that circumvents exposure to virus, mouse cells, or serum. Trypsinization and propagation of HuES9 or H1 hESCs in feeder- and serum-free selection media generated three polyclonal, karyotypically stable, and phenotypically MSC-like cultures that do not express pluripotency-associated markers but displayed MSC-like surface antigens and gene expression profile. They differentiate into adipocytes, osteocytes, and chondrocytes in vitro. Gene expression and fluorescence- activated cell sorter analysis identified CD105 and CD24 as highly expressed antigens on hESC-MSCs and hESCs, respectively. CD105+, CD24- monoclonal isolates have a typical MSC gene expression profiles and were identical to each other with a highly correlated gene expression profile (r 2 > .90). We have developed a protocol to reproducibly generate clinically compliant and identical hESC-MSC cultures. ©AlphaMed Press.link_to_OA_fulltex