Insect (Arthropoda: Insecta) Composition in the Diet of Ornate Box Turtles (Terrapene ornata ornata) in Two Western Illinois Sand Prairies, with a New State Record for Cyclocephala (Coleoptera: Scarabaeidae)

A study of fecal samples collected over a two-year period from juvenile ornate box turtles (Terrapene ornata ornata Agassiz) revealed diets consisting of six orders of insects representing 19 families. Turtles were reared in captivity from eggs harvested from local, wild populations, and released at two remnant prairies. Identifiable insect fragments were found in 94% of samples in 2013 (n=33) and 96% in 2014 (n=25). Frequency of occurrence of insects in turtle feces is similar to results reported in previous studies of midwestern Terrapene species. A comparison of insect composition presented no significant difference between release sites. There is no significant difference in consumed insect species between turtles released into or outside of a fenced enclosure at the same site. Specimens of Cyclocephala longula LeConte collected during this study represent a new state record for Illinois

Stellar and Molecular Radii of a Mira Star: First Observations with the Keck Interferometer Grism

Using a new grism at the Keck Interferometer, we obtained spectrally dispersed (R ~ 230) interferometric measurements of the Mira star R Vir. These data show that the measured radius of the emission varies substantially from 2.0-2.4 microns. Simple models can reproduce these wavelength-dependent variations using extended molecular layers, which absorb stellar radiation and re-emit it at longer wavelengths. Because we observe spectral regions with and without substantial molecular opacity, we determine the stellar photospheric radius, uncontaminated by molecular emission. We infer that most of the molecular opacity arises at approximately twice the radius of the stellar photosphere.Comment: 12 pages, including 3 figures. Accepted by ApJ

Insect (Arthropoda: Insecta) Composition in the Diet of Ornate Box Turtles (Terrapene ornata ornata) in Two Western Illinois Sand Prairies, with a New State Record for Cyclocephala (Coleoptera: Scarabaeidae)

A study of fecal samples collected over a two-year period from juvenile ornate box turtles (Terrapene ornata ornata Agassiz) revealed diets consisting of six orders of insects representing 19 families. Turtles were reared in captivity from eggs harvested from local, wild populations, and released at two remnant prairies. Identifiable insect fragments were found in 94% of samples in 2013 (n=33) and 96% in 2014 (n=25). Frequency of occurrence of insects in turtle feces is similar to results reported in previous studies of midwestern Terrapene species. A comparison of insect composition presented no significant difference between release sites. There is no significant difference in consumed insect species between turtles released into or outside of a fenced enclosure at the same site. Specimens of Cyclocephala longula LeConte collected during this study represent a new state record for Illinois

A New High Contrast Imaging Program at Palomar Observatory

We describe a new instrument that forms the core of a long-term high contrast imaging program at the 200-inch Hale Telescope at Palomar Observatory. The primary scientific thrust is to obtain images and low-resolution spectroscopy of brown dwarfs and young Jovian mass exoplanets in the vicinity of stars within 50 parsecs of the Sun. The instrument is a microlens-based integral field spectrograph integrated with a diffraction limited, apodized-pupil Lyot coronagraph, mounted behind the Palomar adaptive optics system. The spectrograph obtains imaging in 23 channels across the J and H bands (1.06 - 1.78 microns). In addition to obtaining spectra, this wavelength resolution allows suppression of the chromatically dependent speckle noise, which we describe. We have recently installed a novel internal wave front calibration system that will provide continuous updates to the AO system every 0.5 - 1.0 minutes by sensing the wave front within the coronagraph. The Palomar AO system is undergoing an upgrade to a much higher-order AO system ("PALM-3000"): a 3388-actuator tweeter deformable mirror working together with the existing 241-actuator mirror. This system will allow correction with subapertures as small as 8cm at the telescope pupil using natural guide stars. The coronagraph alone has achieved an initial dynamic range in the H-band of 2 X 10^-4 at 1 arcsecond, without speckle noise suppression. We demonstrate that spectral speckle suppression is providing a factor of 10-20 improvement over this bringing our current contrast at an arcsecond to ~2 X 10^-5. This system is the first of a new generation of apodized pupil coronagraphs combined with high-order adaptive optics and integral field spectrographs (e.g. GPI, SPHERE, HiCIAO), and we anticipate this instrument will make a lasting contribution to high contrast imaging in the Northern Hemisphere for years.Comment: Accepted to PASP: 12 pages, 12 figure

Single-cell mRNA transfection studies: Delivery, kinetics and statistics by numbers

AbstractIn artificial gene delivery, messenger RNA (mRNA) is an attractive alternative to plasmid DNA (pDNA) since it does not require transfer into the cell nucleus. Here we show that, unlike for pDNA transfection, the delivery statistics and dynamics of mRNA-mediated expression are generic and predictable in terms of mathematical modeling. We measured the single-cell expression time-courses and levels of enhanced green fluorescent protein (eGFP) using time-lapse microscopy and flow cytometry (FC). The single-cell analysis provides direct access to the distribution of onset times, life times and expression rates of mRNA and eGFP. We introduce a two-step stochastic delivery model that reproduces the number distribution of successfully delivered and translated mRNA molecules and thereby the dose–response relation. Our results establish a statistical framework for mRNA transfection and as such should advance the development of RNA carriers and small interfering/micro RNA-based drugs.From the Clinical EditorThis team of authors established a statistical framework for mRNA transfection by using a two-step stochastic delivery model that reproduces the number distribution of successfully delivered and translated mRNA molecules and thereby their dose-response relation. This study establishes a nice connection between theory and experimental planning and will aid the cellular delivery of mRNA molecules

ANIMA OBSCURA

This technical paper presents the results of the past three years research and development work by an interdisciplinary team of artists, scholars, disability empowerment experts, engineers, computer scientists and medical doctors, working together to invent new cutting edge technologies for real people’s needs. The group has called itself SPIRITlevel (see www.smartlabcentre.com) and has aimed to harness and share the power of technology when invented by and for people with unlimited imaginations despite physical limitations. The project discussed is ‘ANIMA OBSCURA’: a showcase performance designed to demonstrate the empowering abilities of new technology in the performing arts, where bio-affective feedback systems and motion tracking technologies can be usefully (and invisibly) combined with live performance by disabled and able-bodied dancers, to allow every person to dance, move, and bring an augmented world to life. The showcase has broadcastable elements and scaleable format that makes it suitable for demonstration in hospital settings, rehabilitation units, art galleries, museums, and main stages. It can be utilised by individuals for personal therapy and healing, or used collaboratively in online game-playing between hospital beds or, in the ‘mainstream’, through webcast and broadcast formats

Spectral Typing of Late Type Stellar Companions to Young Stars from Low Dispersion Near-Infrared Integral Field Unit Data

We used the Project 1640 near-infrared coronagraph and integral field spectrograph to observe 19 young solar type stars. Five of these stars are known binary stars and we detected the late-type secondaries and were able to measure their JH spectra with a resolution of R\sim30. The reduced, extracted, and calibrated spectra were compared to template spectra from the IRTF spectral library. With this comparison we test the accuracy and consistency of spectral type determination with the low-resolution near-infrared spectra from P1640. Additionally, we determine effective temperature and surface gravity of the companions by fitting synthetic spectra calculated with the PHOENIX model atmosphere code. We also present several new epochs of astrometry of each of the systems. Together these data increase our knowledge and understanding of the stellar make up of these systems. In addition to the astronomical results, the analysis presented helps validate the Project 1640 data reduction and spectral extraction processes and the utility of low-resolution, near-infrared spectra for characterizing late-type companions in multiple systems.Comment: Accepted to Astronomical Journal, 25 pages, 8 figure