Citotoksično djelovanje Ca(OH)2 materijala za punjenje korijenskog kanala na vezivno, koštano i mišićno tkivo

Among numerous root canal sealers those on the basis of Ca(OH)2 are used too. The purpose of this study was to examine possible toxic impact of sealers based on Ca(OH)2 (Sealapex, Kerr and Apexit, Vivadent) on the connective tissue, muscle, bone and cartilage, and compared the results with the impact o f sealers based on the polyketonic compound (Diaket, ESPE). In our experiment 52 female mice of the C-57 BLI6 breed were used. 0.5 ccm of the sealer was injected subcutaneously into the tail root of the laboratory animals. This experimental model was elected due to this location all the examined structures were present. The control group was injected Hanks. The test period was 2, 8 and 30 days respectively, and the sealers impact was histologically examined. After eight days period, the most severe inflammation develops in Sealapex-injected group. After 30 days period in Apexit group, the mildest inflammatory reaction was found. The results indicated Apexit to be the mildest irritant and it had not penetrated into the deeper layers of the tissue.Među mnogim materijalima za punjenje korijenskih kanala danas se sve više upotrebljavaju oni na bazi kalcijevog hidroksida. Željeli smo ispitati mogući toksični učinak materijala na bazi kalcijevog hidroksida (Sealapex, Kerr i Apexit, Vivadent) ipoliketona (Diaket ESPE) na vezivno tkivo, mišiće, kosti i hrskavicu. U korijen repa 52 miša C-57 BL/6 inicirano je 0,5 ccm ispitivanog materijala. Kontrolnoj grupi iniciran je Hanks. Životinje su žrtvovane nakon 2, 8 i 30 dana i napravljeni su histološki preparati. Rezultati pokazuju Apeksit kao najblaži iritans od ispitivanih materijala

Influence of Infection and Trauma on Dentinogenesis

Izvršen je morfološki studij dentinogeneze na incizivima štakora u uvjetima nepovoljnog djelovanja operativne traume u kom binaciji s mono i miješanom infekcijom pulpe s bakterijama Streptococcus mutans i Veillonelle. Na 72 Zgr-Whistar imunogena štakora, podijeljena u pokusnu (54) i kontrolnu grupu (18), inokulirano je, pod aseptičkim uvjetima, na otvorenu pulpu 0,5 McFarlandove koncentracije čiste i miješane kulture bakterija. U kontrolnoj grupi korištena je fiziološka otopina. Nakon 7, 21 i 52 dana pokusnog razdoblja materijal je fiksiran u 10% neutralnoj otopini formalina, smrznut, rezan na debljinu od oko 5 mikrometara i obojen hematoksilin eozinom, a za prikaz bakterija Braun-Breinovom metodom. Rezultati su pokazali da poremećaj mineralizacije dentina može biti posljedica operativne traume i infekcije. Jačina poremećaja odgovarala je patogenosti bakterija, a očitovala se hipomineralizacijom, prolaznom ili progresivnom resorpcijom. Najjače oštećenje u obliku resorptivnih inkluzija u dentinu očitovalo se kod miješane infekcije u 52-dnevnom pokusnom razdoblju.A morphological study o f dentinogenesis was done on rats incisors under conditions o f unfavorable operative trauma acting in combination with mono and mixed pulp infection with the bacteria: Streptococcus mutans and Veillonella. In a group o f 72 Zgr - Whistar imunogene rats, divided into a test (54) and control (18) group, 0,5 McFarland concentrate o f p u re and mixed bacteria culture was inoculated into open pulp under aseptic conditions. Saline solution was used in the control group. After 7, 21 and 52 day-test-period the material was fixed in a 10% neutral formaline solution, frozen, cut app. 5 micrometers thick, and stained with H E for the purpose o f bacteria being shown by the Brown-Brain method. The results show that dentine mineralisation disturbance may be due to operative trauma and infection. The disturbance extent was proportional to bacteria pathogenecity and was manifested through hypomineralisation, temporary or progressive resorption. The strongest mutilation in a form o f resorptive inclusions in the dentine was manifested in the 52-day-test-period mixed infection

Synthesis, Structural Studies and Antitumoral Evaluation of C-6 Alkyl and Alkenyl Side Chain Pyrimidine Derivatives

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