Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits

The aim of this study is to develop a sensitive HPLC method for the quantitative determination of cytisine in serum and to characterize the pharmacokinetic behaviour of cytisine after oral and intravenous administration in rabbits. The pharmacokinetic behaviour of cytisine is studied in male and female New Zealand rabbits after oral and intravenous administration. Cytisine is administered orally (dose of 5 mg/kg b.w.) under fasting condition (12 hours) and intravenously (dose 1 mg/kg b.w.) in the marginal ear vein. Cytisine serum concentrations are measured using a highly selective and sensitive validated HPLC method with UV detection. Linearity of the method is in the range 12–2 400 µg/L; accuracy and precision are both within ± 10%, and the limit of detection is 4 µg/L. Selectivity and stability are also validated. Basic pharmacokinetic parameters of cytisine after single oral and intravenous administration are calculated using TOPFIT software. Pharmacokinetic analysis suggests a rapid but incomplete absorption of cytisine after oral administration

Tobacco policies in Nazi Germany: not as simple as it seems.

OBJECTIVE: Reluctance to develop effective tobacco control measures in Germany has been attributed to the anti-smoking stance taken by the Nazis, which has encouraged pro-smoking groups to equate tobacco control advocacy with totalitarianism. This paper reassesses the scale and nature of tobacco control in Germany during the Third Reich. STUDY DESIGN AND METHODS: Analysis of documents and reports about the situation in Germany in the 1930s and 1940s supplemented by a review of Reich legal ordinances, party newspapers, health behaviour guidelines issued by Nazi party organizations, and interviews with expert informants. RESULTS: While there was considerable opposition to smoking in Nazi Germany, there was no consistent Nazi policy to combat smoking, and what did exist built on pre-existing policies. Although extreme measures were taken in isolated localities or by overzealous party members, there was a marked ambivalence to tobacco control at the highest levels. Many policies were contradictory; measures were often not enforced, and cigarettes were actively distributed to 'deserving' groups. CONCLUSION: Policies on tobacco in Nazi Germany are much more complex than is often represented by those who invoke them to condemn those seeking to reduce the burden of disease caused by smoking