305 research outputs found
Validation of GUt Low Density Array (GULDA), a novel qPCR approach to the study of the intestinal microbial ecosystem
Effects of bacteria used for biopreservation of food on the composittion of intestinal bacteria
Fatty acid composition and phospholipid types used in infant formulas modifies the establishment of human gut bacteria in germ-free mice
AbstractHuman milk fat contains high concentrations of medium-chained fatty acids (MCFA) and triacylglycerols emulsified by a sphingomyelin-rich phospholipid membrane (milk phospholipids, MPL). Infant formula comprises mainly long-chained fatty acids (LCFA) emulsified with dairy proteins and soy lecithin (SL) lacking sphingomyelin. Sphingomyelin content and saturation level of phospholipids affect the gut lipase activity, which alters the concentrations of lipid hydrolysis products in ileum and colon, and hereby putatively affects the competitive advantage of specific gut bacteria. Thus, differences in phospholipid and FA composition may modulate the establishment of the gut microbiota. We investigated effects of fatty acid (FA) composition and emulsification (MPL vs SL) ingested during establishment of human gut microbiota in germ-free mice, and found that cecal microbiotas from mice given MCFA-rich emulsions were characterized by high relative abundances of Bacteroidaceae and Desulfovibrionaceae, while LCFA-rich emulsions caused higher abundances of Enterobacteriaceae, Erysipelotrichaceae, Coriobacteriaceae and Enterococcaceae. Consumption of SL-emulsified lipids skewed the community towards more Enterococcaceae and Enterobacteriaceae, while MPL increased Bacteroidaceae, Desulfovibrionaceae, Rikkenellaceae and Porphyromonadaceae. Intake of SL increased cecal concentrations of iso-valeric and iso-butyric acids. This suggests that fat-type and emulsifiers applied in infant formula may have distinct effects on the establishment of the gut microbiota in formula-fed infants.</jats:p
Effects of apples and specific apple components on the cecal environment of conventional rats: role of apple pectin
<p>Abstract</p> <p>Background</p> <p>Our study was part of the large European project ISAFRUIT aiming to reveal the biological explanations for the epidemiologically well-established health effects of fruits. The objective was to identify effects of apple and apple product consumption on the composition of the cecal microbial community in rats, as well as on a number of cecal parameters, which may be influenced by a changed microbiota.</p> <p>Results</p> <p>Principal Component Analysis (PCA) of cecal microbiota profiles obtained by PCR-DGGE targeting bacterial 16S rRNA genes showed an effect of whole apples in a long-term feeding study (14 weeks), while no effects of apple juice, purée or pomace on microbial composition in cecum were observed. Administration of either 0.33 or 3.3% apple pectin in the diet resulted in considerable changes in the DGGE profiles.</p> <p>A 2-fold increase in the activity of beta-glucuronidase was observed in animals fed with pectin (7% in the diet) for four weeks, as compared to control animals (P < 0.01). Additionally, the level of butyrate measured in these pectin-fed animal was more than double of the corresponding level in control animals (P < 0.01). Sequencing revealed that DGGE bands, which were suppressed in pectin-fed rats, represented Gram-negative anaerobic rods belonging to the phylum <it>Bacteroidetes</it>, whereas bands that became more prominent represented mainly Gram-positive anaerobic rods belonging to the phylum <it>Firmicutes</it>, and specific species belonging to the <it>Clostridium </it>Cluster XIVa.</p> <p>Quantitative real-time PCR confirmed a lower amount of given <it>Bacteroidetes </it>species in the pectin-fed rats as well as in the apple-fed rats in the four-week study (P < 0.05). Additionally, a more than four-fold increase in the amount of <it>Clostridium coccoides </it>(belonging to Cluster XIVa), as well as of genes encoding butyryl-coenzyme A CoA transferase, which is involved in butyrate production, was detected by quantitative PCR in fecal samples from the pectin-fed animals.</p> <p>Conclusions</p> <p>Our findings show that consumption of apple pectin (7% in the diet) increases the population of butyrate- and β-glucuronidase producing <it>Clostridiales</it>, and decreases the population of specific species within the <it>Bacteroidetes </it>group in the rat gut. Similar changes were not caused by consumption of whole apples, apple juice, purée or pomace.</p
Dietary carbohydrate sources affects intestinal microbiota and shorct-chain fatty acid composittion in rats
Dietary carbohydrate source influences molecular fingerprints of the rat faecal microbiota
BACKGROUND: A study was designed to elucidate effects of selected carbohydrates on composition and activity of the intestinal microbiota. Five groups of eight rats were fed a western type diet containing cornstarch (reference group), sucrose, potato starch, inulin (a long- chained fructan) or oligofructose (a short-chained fructan). Fructans are, opposite sucrose and starches, not digestible by mammalian gut enzymes, but are known to be fermentable by specific bacteria in the large intestine. RESULTS: Animals fed with diets containing potato starch, or either of the fructans had a significantly (p < 0.05) higher caecal weight and lower caecal pH when compared to the reference group, indicating increased fermentation. Selective cultivation from faeces revealed a higher amount of lactic acid bacteria cultivable on Rogosa agar in these animals. Additionally, the fructan groups had a lower amount of coliform bacteria in faeces. In the inulin and oligofructose groups, higher levels of butyrate and propionate, respectively, were measured. Principal Component Analysis of profiles of the faecal microbiota obtained by Denaturing Gradient Gel Electrophoresis (DGGE) of PCR amplified bacterial 16S rRNA genes as well as of Reverse Transcriptase-PCR amplified bacterial 16S rRNA resulted in different phylogenetic profiles for each of the five animal groups as revealed by Principal Component Analysis (PCA) of band patterns. CONCLUSION: Even though sucrose and cornstarch are both easily digestible and are not expected to reach the large intestine, the DGGE band patterns obtained indicated that these carbohydrates indeed affected the composition of bacteria in the large gut. Also the two fructans resulted in completely different molecular fingerprints of the faecal microbiota, indicating that even though they are chemically similar, different intestinal bacteria ferment them. Comparison of DNA-based and RNA-based profiles suggested that two species within the phylum Bacteroidetes were not abundant in numbers but had a particularly high ribosome content in the animals fed with inulin
The effect of whole-grain compared to refined wheat on the gut microbial composition and integrity in a colonic epithelial cell model following a 12-week energy-restricted dietary intervention in postmenopausal women
Oxygen restriction increases the infective potential of Listeria monocytogenes in vitro in Caco-2 cells and in vivo in guinea pigs
<p>Abstract</p> <p>Background</p> <p>Listeria monocytogenes has been implicated in several food borne outbreaks as well as sporadic cases of disease. Increased understanding of the biology of this organism is important in the prevention of food borne listeriosis.</p> <p>The infectivity of <it>Listeria monocytogenes </it>ScottA, cultivated with and without oxygen restriction, was compared in <it>vitro </it>and <it>in vivo</it>. Fluorescent protein labels were applied to allow certain identification of <it>Listeria </it>cells from untagged bacteria in <it>in vivo </it>samples, and to distinguish between cells grown under different conditions in mixed infection experiments.</p> <p>Results</p> <p>Infection of Caco-2 cells revealed that <it>Listeria </it>cultivated under oxygen-restricted conditions were approximately 100 fold more invasive than similar cultures grown without oxygen restriction. This was observed for exponentially growing bacteria, as well as for stationary-phase cultures.</p> <p>Oral dosage of guinea pigs with <it>Listeria </it>resulted in a significantly higher prevalence (p < 0.05) of these bacteria in jejunum, liver and spleen four and seven days after challenge, when the bacterial cultures had been grown under oxygen-restricted conditions prior to dosage. Additionally, a 10–100 fold higher concentration of <it>Listeria </it>in fecal samples was observed after dosage with oxygen-restricted bacteria. These differences were seen after challenge with single <it>Listeria </it>cultures, as well as with a mixture of two cultures grown with and without oxygen restriction.</p> <p>Conclusion</p> <p>Our results show for the first time that the environmental conditions to which <it>L. monocytogenes </it>is exposed prior to ingestion are decisive for its <it>in vivo </it>infective potential in the gastrointestinal tract after passage of the gastric barrier. This is highly relevant for safety assessment of this organism in food.</p
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