A detailed questionnaire for the evaluation of health management in dairy sheep and goats

The objective of this work was to develop, use and present a detailed questionnaire for the evaluation of health management in dairy small ruminants; it includes 442 questions organised in seven sections: general, infrastructure, animals, production characteristics, health management, nutrition, human resources. Consistency of replies was evaluated in 27 farmers, interviewed twice. Inconsistent replies were given by all farmers to 30 different questions (Cronbach’s coefficient alpha: 0.987). Then, interviews were performed in 444 farms around Greece. Mean duration of an interview was 63.6 min. Clarifications were requested by 273 farmers to 22 different questions (maximum per farmer: 8). The experience of the investigator, the primary language of farmers and asking clarifications by the farmers affected the duration of the interview. The questionnaire can be used for research work in the field, to record details in the farms under study. In accord with the needs of a particular study, it can be modified, by adding more specific questions or omitting others deemed of less importance. Moreover, it can also be used for routine monitoring purposes, as a useful means to record and maintain details of farms during clinical work. To the best of our knowledge, the questionnaire is the most extensive and detailed one available internationally for dairy small ruminants

Dairy sheep and goat farmers: Socio-demographic characteristics and their associations with health management and performance on farms

The objectives were to determine the socio-demographic profiles of small ruminant dairy farmers and to study associations with management practices, production outcomes and health parameters on their farms. In total, 325 sheep flocks and 119 goat herds across Greece were included in the study and visited for collection of information. Socio-demographic characteristics of the dairy farmers and details of management practices, production outcomes and health parameters on their farms were recorded. For the analysis of results, multivariable models were created using mixed-effects logistic regression, with farms as the random effect. Most dairy farmers were male (93.2%), most were full-time professionals in farming (89.4%) and most had a farming family tradition (86.9%). The mean age was 47.0 years and the mean farming experience was 24.3 years. For 17.3% of the farmers, the highest level of education received was primary education, for 54.3% it was secondary education, for 16.4% it was post-secondary vocational training and for 12.0% it was tertiary education. In 35.4% of dairy farms, external farm workers were employed. Of the sociodemographic characteristics, farming experience was associated with geographical location of farms, management system, breed of animals, application of quarantine measures, laboratory evaluation of feedstuffs, ultrasonographic examination for pregnancy diagnosis, application of vaccination against clostridial infections, means of calculation of bodyweight for drug administration to animals, maintenance of colostrum bank, number of annual veterinary visits, annual milk production per animal, number of newborns and somatic cell counts and total bacterial counts in milk. Further, the employment of external farm workers on the farm was associated with management system, machinemilking, number and breed of animals, application of quarantine measures, laboratory evaluation of feedstuffs, ultrasonographic examination for pregnancy diagnosis, number of annual veterinary visits, annual milk production per animal, protein content in milk and number of newborns. The findings indicate that dairy sheep/goat farming is still a family-driven business, but, nevertheless, there are now younger people among these farmers, many of them with post-secondary education. Socio-demographic characteristics may influence the management practices applied, which in turn can have consequences for production and health results of the farms. © 2021 by the authors. Licensee MDPI, Basel, Switzerland

Scientometrics Study of Research Output on Sheep and Goats from Greece

The study is a scientometrics evaluation of published articles performed in Greece on sheep and goats during the last 25 years, a period coinciding with implementation of reforms to shape and consolidate tertiary education and research establishments in the country. Objectives were: evaluation of the relevant publications and presentation of quantitative characteristics regarding scientific content and bibliometric details. The Web of Science platform was used (search terms: [[sheep OR goat*] OR [Ovis aries OR Capra hircus]] (1997–2022)) and 1080 papers were considered in detail. Throughout the study period, there was a clear progressive increase in numbers of papers published. The papers originated from 39 different entities, most from Aristotle University of Thessaloniki (34.0%), University of Thessaly (28.0%), Agricultural University of Athens (21.2%) and Hellenic Agricultural Organization—Dimitra (13.6%). Papers were published in 318 different journals. Journals with more published papers were Small Ruminant Research and Journal of the Hellenic Veterinary Medical Society. The most frequent general topic of study in the papers was health and welfare (46.7% of papers); second most frequent topic was animal products (18.6%). The papers have received 16,558 citations, i.e., οn average 15.4 citations per paper; the h-index was 56, the i10-index was 518 and the yearly citations per paper were 1.71. Papers on goats had higher impact than papers on sheep. There were 1711 individual authors, of which 728 were first or last authors. In total, 24 authors have each co-authored ≥2.5% of all papers; five authors were each first or last in that proportion of all papers. The findings have indicated that relevant research has focused on milk production and diseases of the udder of small ruminants; moreover, there was accumulation of relevant research in Greece in some establishments only. The findings of the study can be employed to initiate improved relevant research approaches in the country. © 2022 by the authors

Use of Antibiotics Against Bacterial Infections on Dairy Sheep and Goat Farms: Patterns of Usage and Associations with Health Management and Human Resources

The objectives of the study were (a) to describe the patterns of antibiotic usage against four major clinical problems and (b) to evaluate factors that were associated with their use on small ruminant farms. Sheep and goat farmers mostly administered the antibiotics to animals at the dose prescribed (80.4%) and observed the necessary withdrawal period (98.7%), but fewer farmers (22.3%) weighed the animals to calculate their bodyweight before antibiotic administration. For the treatment of clinical mastitis, oxytetracycline, penicillin and streptomycin were the antibiotics used more frequently; 2.03 different antibiotics were used per sheep flock and 2.06 per goat herd, most frequently administered in injectable forms (88.8% of farms). In cases of abortion, oxytetracycline was administered more frequently; 1.12 different antibiotics were used per sheep flock and 1.03 per goat herd. In 94 farms (21.2%), routine administration of antibiotics was performed to newborns; oxytetracycline and ampicillin were administered more often. For the treatment of pneumonia in newborns, oxytetracycline, penicillin and tulathromycin were used more frequently; 1.33 antibiotics were used per sheep flock and 1.29 per goat herd. For the treatment of diarrhoea in lambs and kids, oxytetracycline, amoxicillin and penicillin were the antibiotics used more frequently; 1.34 antibiotics were used per sheep flock and 1.59 per goat herd. Results of multivariable analyses indicated 16 variables associated with the various outcomes for usage of antibiotics for the treatment of the above clinical problems. Of these, 11 variables were associated with the farmers: education of farmers was significant for three outcomes; the age, the experience, the professional involvement and farming family tradition of farmers and the daily period spent at the farm were each significant for one outcome. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Scientometrics approach to research in ovine mastitis from 1970 to 2019 (With a complete list of relevant literature references)

The present study is a scientometrics evaluation of refereed publications on bacterial mastitis in sheep; the objectives were the evaluation of the relevant papers and the presentation of quantitative characteristics regarding their scientific content and bibliometric details. The Web of Science platform was used with search terms: [mastitis OR *mammary infection*] AND [sheep OR ewe* OR ovine] for papers from 1970 to 2019; only ‘articles’, ‘reviews’, ‘proceedings papers’, or ‘data papers’ were evaluated, whilst documents related solely to contagious agalactia, mammary aspects of lentiviral infections, or infections of the teats and the udder skin were excluded. Finally, 580 papers were considered in detail. The number of published papers increased from 8 during the 1970s to 273 during the 2010s. These papers originated from 43 countries (most from Greece or Spain, n = 87 from each) and 240 institutions (145 universities and 95 other establishments), of which 35 produced ≥ 5 papers each. Most papers present original studies (n = 539) with a few reviews (n = 41). The original papers refer to dairy (n = 428), meat (n = 113), or wool (n = 1) production systems and present field (n = 329), laboratory (n = 163), or experimental (n = 67) work; the papers report aetiology (n = 146), risk factors (n = 100), pathogenesis (n = 92), diagnosis (n = 88), effects (n = 66), treatment (n = 50), control (n = 36), or descriptive epidemiology (n = 32) of the disease. Papers related to dairy production present more field and fewer experimental work than papers related to meat production; also, in papers describing work performed in dairy sheep, studies about aetiology, risk factors, and diagnosis of the disease predominate, whilst in papers performed in meat sheep, studies about aetiology, pathogenesis, and effects/diagnosis are reported more often. The papers were published in 175 scientific journals (most in Small Ruminant Research, n = 90, or Journal of Dairy Science, n = 54). On average, the papers received 16.8 total citations and 1.6 yearly citations (h-index = 47). Most papers were published in Scimago classification Q1 (n = 240) or Q2 (n = 230) journals and received 23.4 or 15.4 total citations, respectively. Reviews received more citations than original papers; among the latter, papers with work referring to dairy production received more yearly citations than papers referring to meat production; no differences in citations were seen according to type of work or mastitis aspect covered. Most citations were received by papers from France. Papers published in Journal of Dairy Science or Small Ruminant Research received the most citations. In total, there were 1558 individual authors of the papers, with 24 authors having co-authored > 10 papers each (max: 73 papers); on average, there were 5.2 co-authors per paper (min–max: 1–25). Average number of co-authors progressively increased from 2.1 in the 1970s to 6.3 in the 2010s, with original papers having a higher number of co-authors than reviews: 5.3 and 3.7, respectively. Papers from France had highers number of co-authors (7.9). The findings of this first ever scientometrics study into ovine mastitis indicate that the disease has not been studied as other sheep diseases and that future studies in it should be directed to its control. © 2020 by the authors. Licensee MDPI, Basel, Switzerland

Long‐Term Climatic Changes in Small Ruminant Farms in Greece and Potential Associations with Animal Health

The objectives of this work were (a) to present the changes in climatic parameters from 1989 to 2019, in 444 locations throughout Greece, where small ruminant farms have been based and (b) to present associations of the changes in the climatic parameters with clinical data related to small ruminant health. Climatic variables (1989–2019) were obtained for 444 locations with small ruminant farms throughout Greece. During this period, significant increases were noted in temper-ature‐related parameters (annually 0.05 °C for average temperature and 0.14 °C for temperature range) and precipitation (annually 0.03 mm). There were significant differences in climatic conditions between locations of farms in accord with the management system applied therein, as well as in accord with the breed of animals on the farms (e.g., higher average temperature in locations with Greek breeds, higher temperature range in locations with imported breeds). There were significant associations of temperature‐related parameters with the annual frequency of cases of neonatal hypothermia seen at a veterinary teaching hospital, as well as with the average proportion of Haemon-chus contortus larvae in faecal samples and the frequency of cases of H. contortus resistance reported by a veterinary parasitology laboratory. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Vaccination against Bacterial Mastitis in Sheep

The objective of this review is to discuss the application of vaccination for the prevention of bacterial mastitis in ewes, performed within the frame of health management schemes in sheep flocks. Mastitis is a multi-faceted infection, caused most often by staphylococci; hence, special emphasis is given to staphylococcal mastitis, also given that most relevant studies refer to vaccinations against that infection. Studies regarding various vaccines have been performed; most studies refer to vaccination by using a vaccine making use of cell-free surface polysaccharides in various vehicles, bacterial unbound cells or bacterial cells embedded in their biofilm matrix. Vaccination against mastitis should be better performed during the final stage of pregnancy to allow protection of ewes from lambing and should be considered as one of many control measures for the prevention of the disease. The expected benefits of mastitis vaccination in sheep flocks include the following: (a) reduced incidence risk of clinical and subclinical mastitis, (b) reduced somatic cell counts, optimum chemical composition, absence of staphylococci in milk, (c) increased milk production, (d) reduced dissemination of mastitis-causing pathogens and (e) reduction of antibiotic use in flocks. © 2022 by the authors

Administration of Vaccines in Dairy Sheep and Goat Farms: Patterns of Vaccination, Associations with Health and Production Parameters, Predictors

This paper reports findings regarding patterns of vaccine usage in sheep and goat farms, in 325 sheep flocks and 119 goat herds throughout Greece. The objectives of the study were (a) to describe the patterns of vaccine administration in small ruminant farms and (b) to highlight factors that were associated with vaccinations in the farms. Vaccination against brucellosis was performed in all farms into the study. Among optional vaccinations, anti-clostridial vaccination was most frequently performed (in 97.8% of farms), followed by vaccination against contagious agalactia, (56.5% of farms), pneumonia (41.2%), chlamydial abortion (38.1%), staphylococcal mastitis (36.0%), and paratuberculosis (9.5%). Vaccinations against pneumonia and staphylococcal mastitis were performed more frequently in sheep flocks, whilst vaccinations against paratuberculosis were performed more frequently in goat herds. On average, 2.8 and 2.7 optional vaccinations (i.e., additionally to vaccination against brucellosis) were performed in sheep and goat farms, respectively. The increased number of vaccines administered was associated with a higher average milk production in the respective farms. There was an association of vaccination against staphylococcal mastitis with a reduced recovery of staphylococci from the bulk-tank raw milk. In multivariable analyses, significant associations of the administration of the various optional vaccines were seen with 15 variables, 11 related to health management practices and 4 related to the demographic characteristics of farmers; the collaboration with a veterinarian, the daily number of milking sessions, and the period spent daily by the farmer at the farm premises were each associated with the administration of vaccines against three infections. © 2022 by the authors

Protozoan Parasites in Adult Dairy Small Ruminants and Potential Predictors for Their Presence in Faecal Samples

There is a scope to study protozoan infections in adult ewes and does, as these animals can act as reservoirs of infection for lambs and kids, for which these pathogens are harmful. The objectives of this study were to describe the prevalence of protozoan infections in faecal samples from adult sheep and goats on dairy farms across Greece and to evaluate farm-related factors potentially associated with the presence of protozoan infections in these animals. A cross-sectional study was performed on 325 sheep and 119 goat farms throughout Greece; faecal samples were collected from ewes and does and processed for the identification of protozoan parasites. Eimeria oocysts were found in faecal samples from 69% of farms (72% of sheep farms and 61% of goat farms), Giardia cysts in samples from 33% of farms (33% of sheep farms and 34% of goat farms) and Cryptosporidium oocysts in samples from 8% of farms (7% of sheep farms and 11% of goat farms). In a multivariable analysis, for the presence of Eimeria in samples from sheep farms, the lack of a designated building for lambs emerged as a significant factor; for the presence of Giardia in samples from goat farms, the availability of a main building for animals emerged as a significant factor; for the presence of Cryptosporidium, the lack of grazing and the management system emerged as the main significant factors in sheep and goat farms, respectively. Protozoa were found significantly more frequently in samples collected from farms on which farmers considered diarrhoea as an important health problem in their lambs/kids. © 2022 by the authors

Patterns of Reproductive Management in Sheep and Goat Farms in Greece

This paper reports findings regarding patterns of reproductive management in 325 sheep flocks and 119 goat herds throughout Greece. The objectives were to describe the patterns of reproductive management in sheep and goat farms, to highlight factors that were associated with these management patterns and the clinical application of the various reproductive management approaches in the farms and to establish potential associations with production outcomes. The median months of the start of the mating period for adult sheep and goats were May and June, respectively and for ewe lambs and replacement goats these were August and September, respectively. The median duration of the mating period was 2 months for adults and 1 month for replacements. The median ratio of female to male animals was 22:1 and 25:1 in sheep and goat farms, respectively. Control of reproduction was applied in 33.2% and 16.8% of sheep and goat farms, respectively, mostly by intravaginal progestagen sponges and less often by melatonin implants; multivariable analysis indicated five (availability of milking parlour, number of ewes in a farm, number of daily milkings, daily period spent by farmers at the farm, farming tradition in the family) and two (number of daily milkings, availability of milking parlour) predictors in sheep and goat farms, respectively. Pregnancy diagnosis ultrasonographically was performed in 36.6% and 16.8% of sheep and goat farms, respectively; multivariable analysis indicated as significant three (management system applied in farms, age of farmer, farming tradition in the family) and two (management system applied in farms, availability of milking parlour) predictors in sheep and goat farms, respectively. The control of reproduction and pregnancy diagnosis were associated with a higher number of vaccinations during pregnancy: 2.6 and 2.7, respectively, versus 2.2 and 2.1 in farms where they were not performed. The average per farm number of lambs/kids born per female animal was 1.33 ± 0.01 and 1.30 ± 0.02 for sheep and goat farms, respectively; five (control of reproduction, location of the farm, presence of working staff, collaboration with veterinary practice, breed of ewes) and two (control of reproduction, breed of female goats) predictors were identified in sheep and goat farms, respectively, for high numbers of lambs/kids. Lambs/kids were taken away from their dams at the age of 50 and 65 days, respectively. The average culling age (females, males) was 5.9 and 4.4 years and 6.9 and 4.9 years for sheep and goats, respectively. Farmers sourced animals to be used as replacements for those removed from the farm, mostly from the animals in their own farms, considering criteria related to milk production. Finally, the application of the control of reproduction was associated with increased milk production and the number of newborns, whilst an inverse correlation between the culling age of animals and milk production was seen. © 2022 by the authors