Molecular characterization of KU70 and KU80 homologues and exploitation of a KU70-deficient mutant for improving gene deletion frequency in Rhodosporidium toruloides

10.1186/1471-2180-14-50BMC Microbiology141

Tartronate Semialdehyde Reductase Defines a Novel Rate-Limiting Step in Assimilation and Bioconversion of Glycerol in Ustilago maydis

10.1371/journal.pone.0016438PLoS ONE61

Radio-frequency spectroscopy of a strongly interacting spin-orbit coupled Fermi gas

We investigate experimentally and theoretically radio-frequency spectroscopy and pairing of a spin-orbit-coupled Fermi gas of $^{40}$K atoms near a Feshbach resonance at $B_{0}=202.2$ G. Experimentally, the integrated spectroscopy is measured, showing characteristic blue and red shifts in the atomic and molecular responses, respectively, with increasing spin-orbit coupling. Theoretically, a smooth transition from atomic to molecular responses in the momentum-resolved spectroscopy is predicted, with a clear signature of anisotropic pairing at and below resonance. Our many-body prediction agrees qualitatively well with the observed spectroscopy near the Feshbach resonance.Comment: 7 pages, 4 figures. Supercedes 1302.055

Plant Growth Promoting Abilities of Novel Burkholderia-Related Genera and Their Interactions With Some Economically Important Tree Species

10.3389/fsufs.2021.618305Frontiers in Sustainable Food Systems561830

Disruption of putrescine biosynthesis in shewanella oneidensis enhances biofilm cohesiveness and performance in Cr(VI) immobilization

Although biofilm-based bioprocesses have been increasingly used in various applications, the long-term robust and efficient biofilm performance remains one of the main bottlenecks. In this study, we demonstrated that biofilm cohesiveness and performance of Shewanella oneidensis can be enhanced through disrupting putrescine biosynthesis. Through random transposon mutagenesis library screening, one hyperadherent mutant strain, CP2-1-S1, exhibiting an enhanced capability in biofilm formation, was obtained. Comparative analysis of the performance of biofilms formed by S. oneidensis MR-1 wild type (WT) and CP2-1-S1 in removing dichromate (Cr2O72−), i.e., Cr(VI), from the aqueous phase showed that, compared with the WT biofilms, CP2-1-S1 biofilms displayed a substantially lower rate of cell detachment upon exposure to Cr(VI), suggesting a higher cohesiveness of the mutant biofilms. In addition, the amount of Cr(III) immobilized by CP2-1-S1 biofilms was much larger, indicating an enhanced performance in Cr(VI) bioremediation. We further showed that speF, a putrescine biosynthesis gene, was disrupted in CP2-1-S1 and that the biofilm phenotypes could be restored by both genetic and chemical complementations. Our results also demonstrated an important role of putrescine in mediating matrix disassembly in S. oneidensis biofilms.Published versio

Understanding and exploiting the fatty acid desaturation system in Rhodotorula toruloides

10.1186/s13068-021-01924-yBiotechnology for Biofuels1417

Plant growth promoting abilities of novel Burkholderia-related genera and their interactions with some economically important tree species

A survey of bacterial endophytes associated with the leaves of oil palm and acacias resulted in the isolation of 19 bacterial strains belonging to the genera Paraburkholderia, Caballeronia, and Chitinasiproducens, which are now regarded as distinctively different from the parent genus Burkholderia. Most strains possessed one or more plant growth promotion (PGP) traits although nitrogenase activity was present in only a subset of the isolates. The diazotrophic Paraburkholderia tropica strain S39-2 with multiple PGP traits and the non-diazotrophic Chitinasiproducens palmae strain JS23T with a significant level of 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity were selected to investigate the influence of bacterial inoculation on some economically important tree species. Microscopic examination revealed that P. tropica S39-2 was rhizospheric as well as endophytic while C. palmae JS23T was endophytic. P. tropica strain S39-2 significantly promoted the growth of oil palm, eucalyptus, and Jatropha curcas. Interestingly, the non-diazotrophic, non-auxin producing C. palmae JS23T strain also significantly promoted the growth of oil palm and eucalyptus although it showed negligible effect on J. curcas. Our results suggest that strains belonging to the novel Burkholderia-related genera widely promote plant growth via both N-independent and N-dependent mechanisms. Our results also suggest that the induction of defense response may prevent the colonization of an endophyte in plants.Economic Development Board (EDB)Temasek Foundation CLG LimitedPublished versionThis work was supported by the Temasek Foundation and the Singapore Economy Development Board (EDB)