Characterization and Gene Mapping of an Open-Glume <i>Oryza sativa</i> L. Mutant

Floral organ development determines agricultural productivity by affecting seed development, seed quality, and final yield. In this study, we described the novel ogl mutant in rice (Oryza sativa L.), which is characterized by an open-glume phenotype, increased pistil number, reduced stamen number, decreased seed setting rate, and smaller rice grains. Genetic analysis showed that the open-glume phenotype might be controlled by a recessive qualitative trait locus. Employing bulked segregant analysis (BSA), one candidate region was identified on rice chromosome 1. The glume opening phenotype cosegregated with SNP (Chr1:1522703), which was located at the start codon of one transcript of OsJAG, resulting in partial loss of OsJAG function. cDNA analysis revealed that OsJAG encodes two transcript variants. Compared to normal plants, the expression of OsJAG.1 was upregulated in open-glume plants. When investigating the glume phenotype, we found that the expression of genes related to floral development changed greatly in open-glume plants. Taken together, this work increases our understanding of the developmental role of OsJAG in rice floral development

A 1 &times; 2 Two-Dimensional Slanted Grating Based on Double-Layer Cylindrical Structure

Diffraction gratings play an increasingly important role in various planar optical systems, such as near-eye display systems for virtual reality (VR) and augmented reality (AR). The slanted gratings have more advantages than other elements. A 1 &times; 2 transmission two-dimensional (2D) slanted grating based on a double-layer cylindrical structure was proposed in this paper. In the initial phase of this study, this kind of grating was proposed and designed. We used rigorous coupled-wave analysis (RCWA) and simulated annealing algorithm (SA) to optimize the grating parameters. The effects of the grating geometric parameters on the diffraction efficiency were investigated using rigorous coupled-wave analysis (RCWA). The simulated annealing algorithm (SA) optimization results show that the diffraction efficiency of the (0, &minus;1) and (&minus;1, 0) order exceed 35% under normal incidence in the range of 429&ndash;468 nm wavelength for TE and TM polarization. Meanwhile, the total diffraction efficiency can reach up to 78%. In the last section, we discuss the tolerances for the grating parameters to ensure high quality manufacturing processes. The total effective efficiency is greater than 75% when the MgF2 thickness is from 300 nm to 350 nm and the SiO2 thickness is from 525 nm to 550 nm. Moreover, the grating period has a 53 nm fabrication tolerance, and the slanted angle has a 8.8-degree fabrication tolerance. The relatively large tolerances ensure that it is easy to fabricate the two-dimensional slanted grating and to achieve the targeted objectives. The proposed 2D slanted grating can be applied to 2D exit pupil expansion, which is of great importance in AR/VR applications

Simulation Research on Energy Evolution and Supply Law of Rock–Coal System under the Influence of Stiffness

The energy supply effect caused by the stiffness difference between roofs and sidewalls is an important factor that induces strain coal bursts. In order to quantitatively reveal the energy supply mechanism of strain coal bursts, this paper first establishes a coal burst energy model of the rock–coal system and proposes the calculation formula of coal burst kinetic energy considering supply energy and the stiffness ratio of rock to coal. Then the whole energy evolution law of the rock–coal system with different stiffness ratios is researched by using the numerical simulation method, and the whole process is divided into three stages. With the decrease in the stiffness ratio, the elastic strain energy of the coal changes little, while its kinetic energy is negatively correlated with the stiffness ratio in a power function. Meanwhile, the elastic strain energy and kinetic energy of the rock have power function relations with the stiffness ratio, too. When the rock–coal system is fractured, the kinetic energy of the coal comes from the release of elastic strain energy from the coal and the energy supplied from the rock. The energy supply rate is between 22% and 35% when the stiffness ratio changes from 3.0 to 0.5, and they show a linear relationship, while the supplied energy has a negative power function relationship with the stiffness ratio

A 1 × 2 Two-Dimensional Slanted Grating Based on Double-Layer Cylindrical Structure

Diffraction gratings play an increasingly important role in various planar optical systems, such as near-eye display systems for virtual reality (VR) and augmented reality (AR). The slanted gratings have more advantages than other elements. A 1 × 2 transmission two-dimensional (2D) slanted grating based on a double-layer cylindrical structure was proposed in this paper. In the initial phase of this study, this kind of grating was proposed and designed. We used rigorous coupled-wave analysis (RCWA) and simulated annealing algorithm (SA) to optimize the grating parameters. The effects of the grating geometric parameters on the diffraction efficiency were investigated using rigorous coupled-wave analysis (RCWA). The simulated annealing algorithm (SA) optimization results show that the diffraction efficiency of the (0, −1) and (−1, 0) order exceed 35% under normal incidence in the range of 429–468 nm wavelength for TE and TM polarization. Meanwhile, the total diffraction efficiency can reach up to 78%. In the last section, we discuss the tolerances for the grating parameters to ensure high quality manufacturing processes. The total effective efficiency is greater than 75% when the MgF2 thickness is from 300 nm to 350 nm and the SiO2 thickness is from 525 nm to 550 nm. Moreover, the grating period has a 53 nm fabrication tolerance, and the slanted angle has a 8.8-degree fabrication tolerance. The relatively large tolerances ensure that it is easy to fabricate the two-dimensional slanted grating and to achieve the targeted objectives. The proposed 2D slanted grating can be applied to 2D exit pupil expansion, which is of great importance in AR/VR applications

Continuous Formation Process of CO2 Gas Hydrate via a Vortex and Impinging Stream Reactor

A continuous formation process of CO2 hydrate was investigated ill it vortex and impinging stream reactor (VI R) of 1.76 L with the temperatures ranging front 274.46 to 275.86 K and the pressures ranging from 1.79 to 3.163 MPa. In the VIR, a high-gravity field can be generated, where both centrifugal and impinging forces call intensify the mass transfer ill reactants. There are two distinct steps ill the process. The first step is the Unstable hydrate formation process. The next one is called the stable formation process, which presents the rapid and continuous formation of the CO2 gas hydrate. It is found that the formation rate of CO2 hydrate is Lip to 249.8 mol/h when the Higee factor beta is 390.28

Kinetic studies of gas hydrate formation with low-dosage hydrate inhibitors

Pipeline blockage by gas hydrates is a serious problem in the petroleum industry. Low-dosage inhibitors have been developed for its cost-effective and environmentally acceptable characteristics. In a 1.072-L reactor with methane, ethane and propane gas mixture under the pressure of about 8.5 MPa at 4 A degrees C, hydrate formation was investigated with low-dosage hydrate inhibitors PVP and GHI1, the change of the compressibility factor and gas composition in the gas phase was analyzed, the gas contents in hydrates were compared with PVP and GHI1 added, and the inhibition mechanism of GHI1 was discussed. The results show that PVP and GHI1 could effectively inhibit the growth of gas hydrates but not nucleation. Under the experimental condition with PVP added, methane and ethane occupied the small cavities of the hydrate crystal unit and the ability of ethane entering into hydrate cavities was weaker than that of methane. GHI1 could effectively inhibit molecules which could more readily form hydrates. The ether and hydroxy group of diethylene glycol monobutyl ether have the responsibility for stronger inhibition ability of GHI1 than PVP

Source-sink-flow Characteristics and Super-high Yield Potential of the Super-large-panicle Rice Line DS23 in South China

【Objective】It aims to study the yield performance and source-sink-flow characteristics of new strain of super-large-panicle rice DS23 in early season, and explore the source-flow-sink characteristics and super-high yield potential of DS23, with an aim to provide theoretical basis for high-yield breeding and cultivation of early rice in South China.【Method】The study was conducted under field conditions with the new super-large panicle type strain DS23 as test material and the medium to large panicle type variety YHSM as the control. The leaf area index, SPAD value, accumulation and transport characteristics of non-structural carbohydrate (NSC) in stems, anatomical characteristics of vascular bundles and yield component factors of different panicle types of rice were measured and compared.【Result】The number of grain per panicle, grain weight, sink capacity and yield of DS23 were increased by 37.3%, 4.0%, 33.7% and 15.9% respectively compared to those of YHSM seedlings. The effective number of panicles and seed setting rate were significantly decreased by 6.8% and 15.1% compared to those of YHSM seedlings. There was no significant difference in leaf area index and flag leaf SPAD value between the two varieties at heading stage. Compared with YHSM, total mass of NSC reserved in stem and apparent contribution of transferred NSC to grain yield of DS23 were significantly reduced by 15.3% and 19.8%, respectively, and the apparent transport rate of stem sheath NSC was significantly increased by 10.3%; The DS23 vascular bundle number was significantly increased by 17.3%, and there was no significant difference in the vascular bundle area between the two varieties; DS23 showed no significant change in grain/leaf ratio, with a significant decrease in NSC/spikelet ratio of 34.2% and an increase in vascular bundle load index of 20.4%-60.2%.【Conclusion】The super-large panicle type rice germplasm DS23 planted in early season has a large sink capacity, well-developed vascular bundles transport organization, strong material transformation ability in later stage and great potential for high yield and yield increase. Improvement in seed setting rate is a main direction for exploring its super-high yield potential. The study result lays a theoretical foundation for the breeding of super-large panicle early rice varieties and the research and development of high yield cultivation techniques

Biochemical and molecular characterization of a flavonoid 3-O-glycosyltransferase responsible for anthocyanins and flavonols biosynthesis in Freesia hybrida

The glycosylation of flavonoids increases their solubility and stability in plants. Flowers accumulate anthocyanidin and flavonol glycosides which are synthesized by UDP-sugar flavonoid glycosyltransferases (UFGTs). In our previous study, a cDNA clone (Fh3GT1) encoding UFGT was isolated from Freesia hybrida, which was preliminarily proved to be invovled in cyanidin 3-O-glucoside biosynthesis. Here, a variety of anthocyanin and flavonol glycosides were detected in flowers and other tissues of F. hybrida, implying the versatile roles of Fh3GT1 in flavonoids biosynthesis. To further unravel its multi-functional roles, integrative analysis between gene expression and metabolites was investigated. The results showed expression of Fh3GT1 was positively related to the accumulation of anthocyanins and flavonol glycosides, suggesting its potential roles in the biosynthesis of both flavonoid glycosides. Subsequently, biochemical analysis results revealed that a broad range of flavonoid substrates including flavonoid not naturally occurred in F. hybrida could be recognized by the recombinant Fh3GT1. Both UDP-glucose and UDP-galactose could be used as sugar donors by recombinant Fh3GT1, although UDP-galactose was transferred with relatively low activity. Furthermore, regiospecificity analysis demonstrated that Fh3GT1 was able to glycosylate delphinidin at the 3-, 4'- and 7- positions in a sugar-dependent manner. And the introduction of Fh3GT1 into Arabidopsis UGT78D2 mutant successfully restored the anthocyanins and flavonols phenotypes caused by lost-of-function of the 3GT, indicating that Fh3GT1 functions as a flavonoid 3-O-glucosyltransferase in vivo. In summary, these results demonstrate that Fh3GT1 is a flavonoid 3-O-glycosyltransferase using UDP-glucose as the preferred sugar donor and may involve in flavonoid glycosylation in F. hybrida

The effects of short-term, long-term, and reapplication of biochar on the remediation of heavy metal-contaminated soil

Biochar, a cost-effective amendment, has been reported to play pivotal roles in improving soil fertility and immobilizing soil pollutants due to its well-developed porous structure and tunable functionality. However, the properties of biochar and soils can vary inconsistently after field application. This may affect the remediation of biochar on heavy metal (HM)-contaminated soil being altered. Therefore, we selected lettuce as a model crop to determine the effects of short-term, long-term, and reapplication of biochar on soil physicochemical properties, microbial community, HM bioavailability, and plant toxicity. Our investigation revealed that the long-term application of biochar remarkably improved soil fertility, increased the relative abundance of the phylum Proteobacteria which was highly resistant to HMs, and reduced the abundance of phylum Acidobacteria. These changes in soil properties decreased the accumulation of Cd and Pb in lettuce tissues. The short- and long-term applications of biochar had no substantial effects on biomass, quality, and photosynthesis of lettuce. Moreover, the short-term and reapplication of biochar had no significant effects on soil bacterial communities but decreased the accumulation of Cd and Pb in lettuce tissues. It showed that the changes in the physical, chemical, and biological properties of soil after long-term application of biochar promoted the remediation of HM-contaminated soil. Furthermore, microbial community compositions varied with metal stress and biochar application, while the relative abundance of the phylum Actinobacteria in HM-contaminated soil with long-term biochar application was markedly higher than in HM-contaminated soil without biochar application