Heme oxygenase-1 prevents non-alcoholic steatohepatitis through suppressing hepatocyte apoptosis in mice

<p>Abstract</p> <p>Objective</p> <p>Heme oxygenase-1 (HO-1), the rate-limiting enzyme in heme catabolism, has been reported to have potential antioxidant properties. However, the role of HO-1 on hepatocyte apoptosis remains unclear. We aim to elucidate the effects of HO-1 on oxidative stress related hepatocellular apoptosis in nutritional steatohepatitis in mice.</p> <p>Methods</p> <p>C57BL/6J mice were fed with methionine-choline deficient (MCD) diet for four weeks to induce hepatic steatohepatitis. HO-1 chemical inducer (hemin), HO-1 chemical inhibitor zinc protoporphyrin IX (ZnPP-IX) and/or adenovirus carrying HO-1 gene (Ad-HO-1) were administered to mice, respectively. Hepatocyte apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, the mRNA and protein expression of apoptosis related genes were assayed by quantitative real-time PCR and Western blot.</p> <p>Results</p> <p>Hepatocyte signs of oxidative related apoptotic injury were presented in mice fed with MCD diet for 4 weeks. Induction of HO-1 by hemin or Ad-HO-1 significantly attenuated the severity of liver histology, which was associated with decreased hepatic lipid peroxidation content, reduced number of apoptotic cells by TUNEL staining, down-regulated expression of pro-apoptosis related genes including Fas/FasL, Bax, caspase-3 and caspase-9, reduced expression of cytochrome p4502E1 (CYP2E1), inhibited cytochrome c (Cyt-c) release, and up-regulated expression of anti-apoptosis gene Bcl-2. Whereas, inhibition of HO-1 by ZnPP-IX caused oxidative stress related hepatic injury, which concomitant with increased number of TUNEL positive cells and up-regulated expression of pro-apoptosis related genes.</p> <p>Conclusions</p> <p>The present study provided evidences for the protective role of HO-1 in preventing nutritional steatohepatitis through suppressing hepatocyte apoptosis in mice.</p

Functional stacking of three resistance genes against Phytophthora infestans in potato

Functional stacking of broad spectrum resistance (R) genes could potentially be an effective strategy for more durable disease resistance, for example, to potato late blight caused by Phytophthora infestans (Pi). For this reason, three broad spectrum potato R genes (Rpi), Rpi-sto1 (Solanum stoloniferum), Rpi-vnt1.1 (S. venturii) and Rpi-blb3 (S. bulbocastanum) were selected, combined into a single binary vector pBINPLUS and transformed into the susceptible cultivar Desiree. Among the 550 kanamycin resistant regenerants, 28 were further investigated by gene specific PCRs. All regenerants were positive for the nptII gene and 23 of them contained the three Rpi genes, referred to as triple Rpi gene transformants. Detached leaf assay and agro-infiltration of avirulence (Avr) genes showed that the 23 triple Rpi gene transformants were resistant to the selected isolates and showed HR with the three Avr effectors indicating functional stacking of all the three Rpi genes. It is concluded that Avr genes, corresponding to the R genes to be stacked, must be available in order to assay for functionality of each stack component. No indications were found for silencing or any other negative effects affecting the function of the inserted Rpi genes. The resistance spectrum of these 23 triple Rpi gene transformants was, as expected, a sum of the spectra from the three individual Rpi genes. This is the first example of a one-step approach for the simultaneous domestication of three natural R genes against a single disease by genetic transformation

cobaltironbimetalorganicframeworksasefficientelectrocatalystsfortheoxygenevolutionreaction

The development of high efficiency and stable electrocatalysts for oxygen evolution is critical for energy storage and conversion systems. Herein, a series of Co 2 Fe-MOF exhibited an overpotential of 280 mV at a current density of 10 mA cm ?2 , a low Tafel slope of 44.7 mV dec ?1 , and long-term stability over 12000 s in 1 mol L ?1 KOH. This impressive performance was attributed to the high charge transfer rate, large specific surface area, and synergistic effects of the cobalt and iron centers

Design And Analysis Of Water Supply And Drainage Engineering For A Residential Building

Nowadays, the demand for architecture by the public is not only a living, but also an increase in comfort and safety requirements, and more attention is paid to the design of water supply and drainage works for buildings.In recent years, the rapid development of the economy, whether it is urban or township, the degree of housing community is getting higher and higher, people's requirements for the water supply and drainage function of the building are also constantly improving, which gives the water supply and drainage project a broader development space.In this paper, the nine-story building in a certain district is taken as the research object, and the design analysis is carried out according to the relevant data of the building and the living needs of the residents to verify the feasibility.According to the four parts of water supply, drainage, fire protection and rainwater drainage, the water supply and drainage project of the building is analyzed, and the construction points are briefly given.It can effectively improve the design level and quality of the water supply and drainage system of the building, and facilitate the use of domestic water for residents

The Urban Heat Island Analysis of Changsha-zhuzhou-xiangtan Urban Agglomeration Aased on Modis Data

Using a spatial resolution of MODIS land 1000m standard products, we can get the Land Surface Temperature.Researching for the Land Surface Temperature including spatial and temporal distribution characteristics influence factors.The results show that Spring,Summer and Autumn temperatures mainly concentrated in the central region,Winter temperature mainly concentrated in the South region.From 2001 to 2015,the maximum temperature difference is summer daytime and the difference is 17.58°C,the minimum temperature difference is autumn daytime and the difference is 11.3°C.According to the thermal field intensity distribution,compared 2005 with 2015,Urban Heat Island intensity gradually increased in 2015,the high temperature area increased and distributed more concentrated,and diffusion weakened from the city to the surrounding,the urban heat field is higher than the thermal field.That index by calculating the thermal landscape,account for a dominant position in the middle of heat distribution,and all types index in 2015 are higher than in 2005

Tourism Information Push System Based on Convolutional Neural Network

In the context of the current hot tourism, personalized travel information push is the focus of major travel technology companies. For tourists, an intelligent and humane tourism push system has greatly improved tourism planning. this paper proposes a new tourism push system based on the deep learning technology of the recent hot convolutional neural network. It can satisfy the big data era by acquiring the user's image and text information for convolutional neural network analysis. The intelligent extraction of various network data and personal information and speculation of personal preferences, with a new way of self-learning to reform the current active statistics of the travel push system. The results show that the tourism information pushed by this method is ideal for satisfying the travel preferences of individual users, more humanized and intelligent, and has achieved good results

The Urban Heat Island Analysis of Changsha-zhuzhou-xiangtan Urban Agglomeration Aased on Modis Data

Using a spatial resolution of MODIS land 1000m standard products, we can get the Land Surface Temperature.Researching for the Land Surface Temperature including spatial and temporal distribution characteristics influence factors.The results show that Spring,Summer and Autumn temperatures mainly concentrated in the central region,Winter temperature mainly concentrated in the South region.From 2001 to 2015,the maximum temperature difference is summer daytime and the difference is 17.58°C,the minimum temperature difference is autumn daytime and the difference is 11.3°C.According to the thermal field intensity distribution,compared 2005 with 2015,Urban Heat Island intensity gradually increased in 2015,the high temperature area increased and distributed more concentrated,and diffusion weakened from the city to the surrounding,the urban heat field is higher than the thermal field.That index by calculating the thermal landscape,account for a dominant position in the middle of heat distribution,and all types index in 2015 are higher than in 2005

Integration of human papillomavirus 16 in esophageal carcinoma samples

Abstract Background Esophageal carcinoma (EC) is one of the major cancers in China. In 1982, Syrjanen first hypothesized the relationship between human papillomavirus (HPV) infection and the development of esophageal cancer. Since then, many reports in the field have supported this viewpoint. This study investigated the etiological relationship between HPV infection and the occurrence of esophageal carcinoma at Tangshan City of the Hebei province in China. Methods 189 samples of esophageal carcinoma patients were collected. DNA and RNA were isolated from samples, HPV DNA was detected by polymerase chain reaction (PCR) using My09/11 for HPV L1, and HPV16 was determined using type-specific primer sets for HPV16 E6. The HPV16 integration site was verified by amplification of papillomavirus oncogene transcripts, and HPV16 oncogene transcript products were ligated to the pMD-18 T vector and sequenced to confirm the physical location of HPV16 integration. Results 168 HPV-positive samples were detected in 189 samples, and among them 76 specimens were HPV16 positive. Approximately 600 bp of the HPV16 oncogene transcript were detected in nine esophageal cancer samples. Sequence analysis revealed that HPV16 E7 integrated into human chromosome 2 in three samples, into human chromosome 5 in one sample, into human chromosome 6 in one sample, into human chromosome 8 in two samples, and into human chromosome 17 in two samples. The results verified that the integrated HPV16 E7 in five samples harbored one mutation of viral DNA compared with the HPV16 sequence provided in GenBank (K02718). Conclusions The high prevalence of HPV16 suggests that HPV16 may play an etiological role in the development of esophageal cancer. The integration of HPV16 into host cell chromosomes suggests that persistent HPV infection is key for esophageal epithelial cell malignant transformation and carcinogenesis

Tourism Information Push System Based on Convolutional Neural Network

In the context of the current hot tourism, personalized travel information push is the focus of major travel technology companies. For tourists, an intelligent and humane tourism push system has greatly improved tourism planning. this paper proposes a new tourism push system based on the deep learning technology of the recent hot convolutional neural network. It can satisfy the big data era by acquiring the user's image and text information for convolutional neural network analysis. The intelligent extraction of various network data and personal information and speculation of personal preferences, with a new way of self-learning to reform the current active statistics of the travel push system. The results show that the tourism information pushed by this method is ideal for satisfying the travel preferences of individual users, more humanized and intelligent, and has achieved good results