An evolutionary algorithm with double-level archives for multiobjective optimization

Existing multiobjective evolutionary algorithms (MOEAs) tackle a multiobjective problem either as a whole or as several decomposed single-objective sub-problems. Though the problem decomposition approach generally converges faster through optimizing all the sub-problems simultaneously, there are two issues not fully addressed, i.e., distribution of solutions often depends on a priori problem decomposition, and the lack of population diversity among sub-problems. In this paper, a MOEA with double-level archives is developed. The algorithm takes advantages of both the multiobjective-problemlevel and the sub-problem-level approaches by introducing two types of archives, i.e., the global archive and the sub-archive. In each generation, self-reproduction with the global archive and cross-reproduction between the global archive and sub-archives both breed new individuals. The global archive and sub-archives communicate through cross-reproduction, and are updated using the reproduced individuals. Such a framework thus retains fast convergence, and at the same time handles solution distribution along Pareto front (PF) with scalability. To test the performance of the proposed algorithm, experiments are conducted on both the widely used benchmarks and a set of truly disconnected problems. The results verify that, compared with state-of-the-art MOEAs, the proposed algorithm offers competitive advantages in distance to the PF, solution coverage, and search speed

Neural differentiation of adipose-derived stem cells by indirect co-culture with Schwann cells

To investigate whether adipose-derived stem cells (ADSCs) could be subject to neural differentiation induced only by Schwann cell (SC) factors, we co-cultured ADSCs and SCs in transwell culture dishes. Immunoassaying, Western blot analysis, and RT-PCR were performed (1, 3, 7, 14 d) and the co-cultured ADSCs showed gene and protein expression of S-100, Nestin, and GFAP. Further, qRT-PCR disclosed relative quantitative differences in the above three gene expressions. We think ADSCs can undergo induced neural differentiation by being co-cultured with SCs, and such differentia­tions begin 1 day after co-culture, become apparent after 7 days, and thereafter remain stable till the 14th day