In Death Thy Life is Found : An Examination of the Forgotten Poetry of Margaret Fuller.

Despite the recent scholarship that has been performed on Margaret Fuller, very little has focused on the varied body of poetry she composed during her brief life. By dividing her poetic works into three categories – those written to an early “lover,” those focusing on the theme of androgyny, and those written during her “mature period” of 1844 – one is better able to follow Fuller on the emotional and intellectual journey that served as the foundation for all of her writings. In addition, the study of Fuller’s poetry provides a clearer understanding of how this erudite woman transcended gender boundaries in her writings, as well as in the choices she made in her daily life, further emphasizing her reputation as a revolutionary woman of nineteenth century

Considerations in Promoting Parent and Family Involvement

It has been recognized for decades that parent and family (PF) involvement is a vital component of students\u27 educational experiences. Moreover, PF involvement is identified as an important protective factor for students. Thus, school administrators and educators understanding and encouraging positive relationships between families and schools may be one way to promote academic, social, and emotional success for youth throughout their lives. The purpose of this paper is to examine the literature on PF involvement and delineate a proposed model of PF involvement to foster resilience in children and youth

Conservation policies informed by food system feedbacks can avoid unintended consequences

Understanding the feedbacks between food systems and conservation policies can help avoid unintended environmental consequences. Using a survey-based choice experiment and economic modelling, we quantify the potential impact of tourists’ responses to a shift in offshore fish supply after the designation of a large-scale marine protected area in Palau. We find that this conservation policy may increase offshore fish prices and tourists’ consumption of reef fish, thereby further endangering local reef ecosystems. However, if tourists are offered a sustainable offshore choice, their demand for fish could be kept at current levels, and environmental impacts from increased reef fish consumption would be avoided

Weak Stock Bycatch Avoidance in the West Coast Ocean Salmon Fishery: A Bioeconomic Model Using Spatio-Temporal Data from Commercial Fishermen

The spatio-temporal overlap of morphologically undistinguishable weak and healthy stocks is a major concern for the Pacific Northwest troll Chinook salmon fishery. Regular fishery closures have led to major financial losses calling for alternative regulatory measures. One approach for such complex and pressing socio-ecological challenges is the transition towards transdisciplinary research spanning disciplinary boundaries. This study combines natural and human sciences with stakeholder participation. Based on a unique genetic dataset, fine-scale patterns of fishing effort, stock distributions and spatial stock overlaps were assessed. Two hot spots of weak Klamath Chinook in the Oregon coast regions were identified and related to the topography. This knowledge was used to develop a simple bioeconomic model to evaluate costs and benefits of re-allocating effort to avoid bycatch of the weak stock. The model is a dynamic, spatially-explicit feedback model, composed of sub-modules, running on a monthly basis. A status quo and an alternative scenario of a 50% cut of Klamath catch were tested. The scenarios show that effort re-allocation can lead to reduction in Klamath catch and increasing net profit but outcomes depend on the distance from the home ports to the new fishing area. The output of the model at its current stage should be regarded strategically, providing a qualitative understanding of the types of best strategies. Despite some challenges in transdisciplinarity discussed in this study and the present limitations to incorporate fine-scale changes of Chinook salmon stock distributions in management regulations, this is the direction that fishery management needs to be heading.Proceedings of the Eighteenth Biennial Conference of the International Institute of Fisheries Economics and Trade, held July 11-15, 2016 at Aberdeen Exhibition and Conference Center (AECC), Aberdeen, Scotland, UK

Artificial Intelligence Approaches to Assessing Primary Cilia

Cilia are microtubule based cellular appendages that function as signaling centers for a diversity of signaling pathways in many mammalian cell types. Cilia length is highly conserved, tightly regulated, and varies between different cell types and tissues and has been implicated in directly impacting their signaling capacity. For example, cilia have been shown to alter their lengths in response to activation of ciliary G protein-coupled receptors. However, accurately and reproducibly measuring the lengths of numerous cilia is a time-consuming and labor-intensive procedure. Current approaches are also error and bias prone. Artificial intelligence (Ai) programs can be utilized to overcome many of these challenges due to capabilities that permit assimilation, manipulation, and optimization of extensive data sets. Here, we demonstrate that an Ai module can be trained to recognize cilia in images from both in vivo and in vitro samples. After using the trained Ai to identify cilia, we are able to design and rapidly utilize applications that analyze hundreds of cilia in a single sample for length, fluorescence intensity and co-localization. This unbiased approach increased our confidence and rigor when comparing samples from different primary neuronal preps in vitro as well as across different brain regions within an animal and between animals. Moreover, this technique can be used to reliably analyze cilia dynamics from any cell type and tissue in a high-throughput manner across multiple samples and treatment groups. Ultimately, Ai-based approaches will likely become standard as most fields move toward less biased and more reproducible approaches for image acquisition and analysis

Viability of preloaded Descemet membrane endothelial keratoplasty grafts with 96-hour shipment

Objective To assess feasibility and compare the effects of 96-hour shipment of Descemet membrane endothelial keratoplasty (DMEK) grafts as a scroll or a tri-fold on cell viability.Methods and analysis DMEK grafts were prepared at the Rocky Mountain Lions Eye Bank. Twenty pre-stripped DMEK grafts, paired from 10 donors, were either tri-folded in an endothelium-in configuration using microforceps and loaded into a plastic Treyetech cartridge, or suctioned in a scrolled endothelium-out configuration into a modified Jones Tube. Grafts were shipped via FedEx to a secondary location and back for 48 hours each way, resulting in a total shipping time of 96 hours. After shipping, grafts were removed from inserters onto glass slides and unfolded using viscoelastic with endothelium facing upwards. Calcein-AM stained grafts were imaged with a fluorescent microscope and endothelial cell loss (ECL) was measured using trainable segmentation in Fiji by a masked grader.Results A total of 20 grafts were shipped for 96 hours, split between preloaded tri-folded (n=10) and preloaded scrolled (n=10) tissues. No significant difference in ECL was observed across groups after prolonged shipping (14.8% vs 13.7% ECL respectively, p=0.68).Conclusion For preloaded DMEK after 96 hours, both scrolled and tri-folded tissue demonstrated clinically acceptable levels of ECL. The data suggest a wider window of time for endothelial cell viability and is promising for the prospect of international shipment of preloaded grafts

Formation of Developmentally Toxic Phenanthrene Metabolite Mixtures by <i>Mycobacterium</i> sp. ELW1

<i>Mycobacterium</i> sp. ELW1 co-metabolically degraded up to 1.8 μmol of phenanthrene (PHE) in ∼48 h, and hydroxyphenanthrene (OHPHE) metabolites, including 1-hydroxyphenanthrene (1-OHPHE), 3-hydroxyphenanthrene (3-OHPHE), 4-hydroxyphenanthrene (4-OHPHE), 9-hydroxyphenanthrene (9-OHPHE), 9,10-dihydroxyphenanthrene (1,9-OHPHE), and <i>trans</i>-9,10-dihydroxy-9,10-dihydrophenanthrene (<i>trans</i>-9,10-OHPHE), were identified and quantified over time. The monooxygenase responsible for co-metabolic transformation of PHE was inhibited by 1-octyne. First-order PHE transformation rates, <i>k</i>_PHE, and half-lives, <i>t</i>_1/2, for PHE-exposed cells were 0.16–0.51 h^–1 and 1.4–4.3 h, respectively, and the 1-octyne controls ranged from 0.015–0.10 h^–1 to 7.0–47 h, respectively. While single compound standards of PHE and <i>trans</i>-9,10-OHPHE, the major OHPHE metabolite formed by ELW1, were not toxic to embryonic zebrafish (<i>Danio rerio</i>), single compound standards of minor OHPHE metabolites, 1-OHPHE, 3-OHPHE, 4-OHPHE, 9-OHPHE, and 1,9-OHPHE, were toxic, with effective concentrations (EC₅₀’s) ranging from 0.5 to 5.5 μM. The metabolite mixtures formed by ELW1, and the reconstructed standard mixtures of the identified OHPHE metabolites, elicited a toxic response in zebrafish for the same three time points. EC₅₀s for the metabolite mixtures formed by ELW1 were lower (more toxic) than those for the reconstructed standard mixtures of the identified OHPHE metabolites. Ten unidentified hydroxy PHE metabolites were measured in the derivatized mixtures formed by ELW1 and may explain the increased toxicity of the ELW1 metabolites mixture relative to the reconstructed standard mixtures of the identified OHPHE metabolites

Reach out churches: A community-based participatory research pilot trial to assess the feasibility of a mobile health technology intervention to reduce blood pressure among African Americans

Innovative strategies are needed to reduce the hypertension epidemic among African Americans. Reach Out was a faith-collaborative, mobile health, randomized, pilot intervention trial of four mobile health components to reduce high blood pressure (BP) compared to usual care. It was designed and tested within a community-based participatory research framework among African Americans recruited and randomized from churches in Flint, Michigan. The purpose of this pilot study was to assess the feasibility of the Reach Out processes. Feasibility was assessed by willingness to consent (acceptance of randomization), proportion of weeks participants texted their BP readings (intervention use), number lost to follow-up (retention), and responses to postintervention surveys and focus groups (acceptance of intervention). Of the 425 church members who underwent BP screening, 94 enrolled in the study and 73 (78%) completed the 6-month outcome assessment. Median age was 58 years, and 79% were women. Participants responded with their BPs on an average of 13.7 (SD = 10.7) weeks out of 26 weeks that the BP prompts were sent. All participants reported satisfaction with the intervention. Reach Out, a faith-collaborative, mobile health intervention was feasible. Further study of the efficacy of the intervention and additional mobile health strategies should be considered

Mks6 mutations reveal tissue- and cell type-specific roles for the cilia transition zone

The transition zone (TZ) is a domain at the base of the cilium that is involved in maintaining ciliary compartment-specific sensory and signaling activity by regulating cilia protein composition. Mutations in TZ proteins result in cilia dysfunction, often causing pleiotropic effects observed in a group of human diseases classified as ciliopathies. The purpose of this study is to describe the importance of the TZ component Meckel-Grüber syndrome 6 (Mks6) in several organ systems and tissues regarding ciliogenesis and cilia maintenance using congenital and conditional mutant mouse models. Similar to MKS, congenital loss of Mks6 is embryonic lethal, displaying cilia loss and altered cytoskeletal microtubule modifications but only in specific cell types. Conditional Mks6 mutants have a variable cystic kidney phenotype along with severe retinal degeneration with mislocalization of phototransduction cascade proteins. However, other phenotypes, such as anosmia and obesity, which are typically associated with cilia and TZ dysfunction, were not evident. These data indicate that despite Mks6 being a core TZ component, it has tissue- or cell type-specific functions important for cilia formation and cilia sensory and signaling activities