2 research outputs found
The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence
AKTIP is a shelterin-interacting protein required for replication of telomeric
DNA. Here, we show that AKTIP biochemically interacts with A- and B-type
lamins and affects lamin A, but not lamin C or B, expression. In interphase
cells, AKTIP localizes at the nuclear rim and in discrete regions of the
nucleoplasm just like lamins. Double immunostaining revealed that
AKTIP partially co-localizes with lamin B1 and lamin A/C in interphase
cells, and that proper AKTIP localization requires functional lamin A. In
mitotic cells, AKTIP is enriched at the spindle poles and at the midbody
of late telophase cells similar to lamin B1. AKTIP-depleted cells show senescence-associated markers and recapitulate several aspects of the progeroid
phenotype. Collectively, our results indicate that AKTIP is a new player in
lamin-related processes, including those that govern nuclear architecture,
telomere homeostasis and cellular senescence