THE EFFECT OF SOME PLANT GROWTH REGULATORS AND THEIR COMBINATION WITH METHYL JASMONATE ON ANTHOCYANIN FORMATION IN ROOTS OF KALANCHOE BLOSSFELDIANA

ABSTRACT In this study, we investigated the effect of plant growth regulators (PGRs) -auxins, gibberellin, cytokinin, abscisic acid, brassinosteroid, ethylene and their interaction with methyl jasmonate (JA-Me) applied to roots of the whole plants Kalanchoe blossfeldiana on the accumulation of anthocyanins in roots. The highest stimulation of anthocyanins synthesis was stated with application of JA-Me alone. In response to treatments with the other tested PGRs, the content of anthocyanins in roots of a whole plant was different depending on the concentration of the PGR when being applied alone or together with JA-Me. Auxin, indole-3-acetic acid (IAA) at a concentration of 50 mg·L -1 , indole-3-butyric acid (IBA) at 5 mg·L -1 and abscisic acid (ABA) at 10 mg·L -1 induced anthocyanin accumulation with approximately 60-115% compared to the control while 24-epibrassinolid (epiBL), gibberellic acid (GA3) and 6-benzylaminopurine (BAP) had no effect on the anthocyanin accumulation. The simultaneous administration of the PGRs with JA-Me usually resulted in the accumulation of anthocyanins in roots in a manner similar to that caused by JA-Me. PGRs applied to isolated roots did not stimulate anthocyanin accumulation, except for the combination of JA-Me with 50 mg·L -1 IAA. The results indicate that in K. blossfeldiana, the aboveground parts of the plant play an important role in the biosynthesis of anthocyanins in roots

Differential effects of auxin polar transport inhibitors on rooting in some Crassulaceae species

Effects of auxin polar transport inhibitors, 2,3,5-triio-dobenzoic acid (TIBA), 1-N-naphthylphthalamic acid (NPA) and methyl 2-chloro-9-hydroxyfluorene-9-carboxylate (morphactin IT 3456), as a lanolin paste, on root formation in cuttings of some species of Crassulaceae, such as Bryophyllum daigremontianum, B. calycinum, Kalanchoe blossfeldiana and K. tubiflora, were studied. Cuttings of these plants were easily rooted in water without any treatment. TIBA and morphactin IT 3456 completely inhibited root formation in the cuttings of these plants but NPA did not when these inhibitors were applied around the stem below the leaves. When TIBA and morphactin were applied around the stem near the top, but leaves were present below the treatment, the root formation was observed in B. calycinum and K. blossfeldiana but in a smaller degree than in control cuttings. These results strongly suggest that endogenous auxin is required for root formation in cuttings of Crassulaceae plants. The differential mode of action of NPA is discussed together with its effect on auxin polar transport

Out-of-hospital cardiac arrest: Do we have to perform coronary angiography?

Out-of-hospital cardiac arrest (OHCA) remains a leading cause of global mortality, while survivors are burdened with long-term neurological and cardiovascular complications. OHCA management at the hospital level remains challenging, due to heterogeneity of OHCA presentation, the critical status of OHCA patients reaching the return of spontaneous circulation (ROSC), and the demands of post ROSC treatment. The validity and optimal timing for coronary angiography is one important, yet not fully defined, component of OHCA management. Guidelines state clear recommendations for coronary angiography in OHCA patients with shockable rhythms, cardiogenic shock, or in patients with ST-segment elevation observed in electrocardiography after ROSC. However, there is no established consensus on the angiographic management in other clinical settings. While coronary angiography may accelerate the diagnostic and therapeutic process (provided OHCA was a consequence of coronary artery disease), it might come at the cost of impaired post-resuscitation care quality due to postponing of intensive care management. The aim of the current statement paper is to discuss clinical strategies for the management of OHCA including the stratification to invasive procedures and the rationale behind the risk-benefit ratio of coronary angiography, especially with patients in critical condition

Untersuchung des Xylose- und Arabinose-Stoffwechsels in <i>Clostridium acetobutylicum</i> ATCC 824 und <i>Clostridium saccharobutylicum</i> NCP 262.

Xylulose kinase (xylB) and arabinose kinase (araK) mutants of Clostridium acetobutylicum and C. saccharobutylicum were investigated and compared to wild type strains, regarding their abilities to metabolize xylose, arabinose and glucose. To create mutants in C. saccharobutylicum, an effective triparental mating system for DNA transfer was developed, and additionally restrictase mutants were constructed and characterized. Furthermore, growth and gene expression of C. acetobutylicum in continuous fermentation on xylose were analyzed.Xylulose Kinase (xylB) und Arabinose Kinase (araK) Mutanten von Clostridium acetobutylicum und C. saccharobutylicum wurden mit dem Wildtyp verglichen und auf ihre Fähigkeit untersucht, Xylose, Arabinose und Glucose zu verstoffwechseln. Für die Konstruktion der Mutanten in C. saccharobutylicum wurde ein effektives System für den DNA-Transfer durch triparentale Paarung entwickelt. Zusätzlich wurden Restrictase Mutanten konstruiert und charakterisiert. Darüber hinaus wurde das Wachstum und die Geneexpression von C. acetobutylicum in kontinuierlicher Kultur auf Xylose analysiert

Skuteczny duet w terapii miejscowej łuszczycy — połączenie kalcypotriolu z dipropionianem betametazonu w tradycyjnej formie maści

Exclusive local therapy is a sufficient treatment option for most mild cases of psoriasis. It is estimated that even 70–80% of patients do not require systemic treatment or light therapy, and topical medications effectively control the symptoms of the disease. Moreover, topical medications are also an essential treatment option in addition to systemic therapy for moderate to severe psoriasis. The most common medications used in local therapy are glucocorticosteroids and vitamin D analogues. These substances show the highest efficiency among all drugs available on the market, while maintaining a favorable toxicity profile. A significant advantage of these medications is also the ease of use, which significantly affects the compliance with medical recommendations and regular use of the preparation by patients. The different mechanism of action of the two drugs makes this unique combination of calcipotriol and betamethasone dipropionate particularly effective at any stage of the pathogenesis of psoriasis. Due to their strong antiproliferative, immunomodulating and immunostatic effects, this duo is currently recommended by the Polish Dermatological Society as a first-line preparation in the local treatment of smooth skin, hands and feet. The ointment ensures rapid penetration of active substances, strongly moisturizing, reducing skin atrophy, which is an additional benefit for patients with psoriasis. The study analyzes the effectiveness, safety and mechanism of action of medications containing calcipotriol with betamethasone dipropionate in the local therapy of psoriasis in the light of current literature data, with particular emphasis on the form of ointment.Wyłączna terapia miejscowa jest wystarczającą opcją leczenia w większości łagodnych postaci łuszczycy. Szacuje się, że nawet 70–80% chorych nie wymaga leczenia ogólnoustrojowego czy terapii światłem, a preparaty miejscowe skutecznie kontrolują objawy choroby. Ponadto leki stosowane miejscowo stanowią również niezbędną metodę leczenia, która jest uzupełnieniem terapii ogólnoustrojowej łuszczycy o nasileniu umiarkowanym i ciężkim. Najczęstszymi preparatami stosowanymi w terapii miejscowej są glikokortykosteroidy oraz analogi witaminy D. Substancje te wykazują najwyższą skuteczność spośród wszystkich dostępnych na rynku leków, przy zachowaniu korzystnego profilu toksyczności. Znacznym atutem tych preparatów jest ponadto łatwość ich użycia, co istotnie wypływa na przestrzeganie zaleceń lekarskich i regularne stosowanie preparatu przez pacjentów. Odmienny mechanizm działania obu tych leków sprawia, że to wyjątkowe połączenie kalcypotriolu z dipropionianem betametazonu jest szczególnie skuteczne na każdym etapie patogenezy łuszczycy. Silne działanie antyproliferacyjne, immunomodulujące i immunostatyczne sprawia, że duet ten jest aktualnie rekomendowany przez Polskie Towarzystwo Dermatologiczne jako preparat pierwszego rzutu w leczeniu miejscowym łuszczycy skory gładkiej, dłoni i stop. Forma preparatu w postaci maści zapewnia szybką penetrację substancji aktywnych, działając dodatkowo silnie nawilżająco, redukując atrofię skory, co stanowi dodatkową korzyść dla pacjentów z łuszczycą. W pracy dokonano analizy skuteczności, bezpieczeństwa oraz mechanizmu działania preparatów zawierających kalcypotriol z dipropionianem betametazonu w miejscowej terapii łuszczycy w świetle aktualnych danych literaturowych ze szczególnym uwzględnieniem formy maści

The Effect Of Some Plant Growth Regulators And Their Combination With Methyl Jasmonate On Anthocyanin Formation In Roots Of Kalanchoe Blossfeldiana

In this study, we investigated the effect of plant growth regulators (PGRs) - auxins, gibberellin, cytokinin, abscisic acid, brassinosteroid, ethylene and their interaction with methyl jasmonate (JA-Me) applied to roots of the whole plants Kalanchoe blossfeldiana on the accumulation of anthocyanins in roots. The highest stimulation of anthocyanins synthesis was stated with application of JA-Me alone. In response to treatments with the other tested PGRs, the content of anthocyanins in roots of a whole plant was different depending on the concentration of the PGR when being applied alone or together with JA-Me. Auxin, indole-3-acetic acid (IAA) at a concentration of 50 mg·L-1, indole-3-butyric acid (IBA) at 5 mg·L-1 and abscisic acid (ABA) at 10 mg·L-1 induced anthocyanin accumulation with approximately 60-115% compared to the control while 24-epibrassinolid (epiBL), gibberellic acid (GA3) and 6-benzylaminopurine (BAP) had no effect on the anthocyanin accumulation. The simultaneous administration of the PGRs with JA-Me usually resulted in the accumulation of anthocyanins in roots in a manner similar to that caused by JA-Me. PGRs applied to isolated roots did not stimulate anthocyanin accumulation, except for the combination of JA-Me with 50 mg·L-1 IAA

Śródskórne iniekcje acetonidu triamcynolonu w leczeniu Zespołu Grahama-Little’a — opis przypadku

Graham-Little-Piccardi-Lasseur syndrome (GLPLS) is a rare lichen planopillaris defined by scarring alopecia, loss of pubic and axillary hairs and progressive development of follicular papules located on the whole body. Topical or systemic corticosteroids, cysclosporine or hydroxychlorquina are the treatments usually proposed. We describe the effectiveness of intralesional triamcinolone acetonide in concentration of 8 mg/mL with oral acitretin 10 mg/day combined with topical 0.05% clobetasol propionate ointment in a patient with GLPLS. After 2 months of treatment, reduction of perifollicular erythema and follicular hyperkeratotic papules were observed with no new area of alopecia. We believe that intralesional triamcinolone acetonide could be an effective treatment by limiting the progression of the disease.Zespół Grahama-Little’a-Piccardiego-Lasseura (GLPLS, Graham-Little-Piccardi-Lasseur syndrome) jest rzadką postacią liszaja płaskiego mieszkowego, objawiającego się postępującym łysieniem bliznowaciejącym skóry owłosionej głowy, niebliznowaciejącą utratą włosów w okolicy pach i pachwin oraz licznymi grudkami przymieszkowymi z hiperkeratozą na skórze całego ciała. W terapii stosowane są zarówno miejscowe, jak i systemowe glikokortykosteroidy (GKS), cyklosporyna czy hydroksychlorchina. W artykule opisujemy skuteczność leczenia GLPLS iniekcjami śródskórnymi acetonidu triamcynolonu w stężeniu 8 mg/ml, doustną acytretyną 10 mg/d oraz maścią z 0,05% propionianem klobetazolu. Po 2 miesiącach terapii przymieszkowy rumień i hiperkeratotyczne grudki uległy poprawie oraz nie stwierdzono nowych ognisk łysienia. Uważamy, że śródskórne iniekcje acetonidu triamcynolonu mogą okazać się skutecznie w zahamowaniu procesu chorobowego

Stochastic operation model for readiness assessment of small wind turbines based on Markov theory

Abstract Wind energy is now one of the most important alternative sources of renewable energy. The development of wind power generation is a prerequisite to meet environmental and sustainable development requirements while achieving energy security goals. The condition for the effectiveness of the proper operation of a wind power plant is its ability to perform the intended functions, which can be expressed by the coefficient of technical readiness. This article presents an original analysis of the readiness of small wind turbines. As part of the research, an original stochastic operation model was developed based on the application of the Markov process theory. Based on the collected data, a 3‐state phase space of the studied process was identified. Various analyses were carried out to calculate the operating coefficients of wind power plants. The ergodic (boundary) probabilities were calculated using three methods. The conditional probabilities were then determined based on the initial distribution. The resulting readiness factors, not exceeding 30%, indicate the low operational efficiency of the small wind turbines in question. Furthermore, an analysis of the sensitivity of the performance factors was carried out for the analysed objects