10 research outputs found
Ultrastructure and composition of cell wall appositions in the roots of **Asplenium** (Polypodiales)
Annular dark-field transmission electron microscopy for low contrast materials
AbstractImaging soft matter by transmission electron microscopy (TEM) is anything but straightforward. Recently, interest has grown in developing alternative imaging modes that generate contrast without additional staining. Here, we present a dark-field TEM technique based on the use of an annular objective aperture. Our experiments demonstrate an increase in both contrast and signal-to-noise ratio in comparison to conventional bright-field TEM. The proposed technique is easy to implement and offers an alternative imaging mode to investigate soft matter.</jats:p