The Effective Energy-Momentum Tensor in Kaluza-Klein Gravity With Large Extra Dimensions and Off-Diagonal Metrics

We consider a version of Kaluza-Klein theory where the cylinder condition is not imposed. The metric is allowed to have explicit dependence on the "extra" coordinate(s). This is the usual scenario in brane-world and space-time-matter theories. We extend the usual discussion by considering five-dimensional metrics with off-diagonal terms. We replace the condition of cylindricity by the requirement that physics in four-dimensional space-time should remain invariant under changes of coordinates in the five-dimensional bulk. This invariance does not eliminate physical effects from the extra dimension but separates them from spurious geometrical ones. We use the appropriate splitting technique to construct the most general induced energy-momentum tensor, compatible with the required invariance. It generalizes all previous results in the literature. In addition, we find two four-vectors, J_{m}^{mu} and J_{e}^{mu}, induced by off-diagonal metrics, that separately satisfy the usual equation of continuity in 4D. These vectors appear as source-terms in equations that closely resemble the ones of electromagnetism. These are Maxwell-like equations for an antisymmetric tensor {F-hat}_{mu nu} that generalizes the usual electromagnetic one. This generalization is not an assumption, but follows naturally from the dimensional reduction. Thus, if {F-hat}_{mu nu} could be identified with the electromagnetic tensor, then the theory would predict the existence of classical magnetic charge and current. The splitting formalism used allows us to construct 4D physical quantities from five-dimensional ones, in a way that is independent on how we choose our space-time coordinates from those of the bulk.Comment: New title, editorial changes made as to match the version to appear in International Journal of Modern Physics

FRW Cosmology From Five Dimensional Vacuum Brans-Dicke Theory

We follow approach of induced matter theory for 5D vacuum BD, introduce induced matter and potential in 4D hypersurfaces, and employ generalized FRW type solution. We confine ourselves to scalar field and scale factors be functions of the time. This makes the induced potential, by its definition, vanishes. When the scale factor of fifth dimension and scalar field are not constants, 5D eqs for any geometry admit a power law relation between scalar field and scale factor of fifth dimension. Hence the procedure exhibits that 5D vacuum FRW like eqs are equivalent, in general, to corresponding 4D vacuum ones with the same spatial scale factor but new scalar field and coupling constant. We show that 5D vacuum FRW like eqs or its equivalent 4D vacuum ones admit accelerated solutions. For constant scalar field, eqs reduce to usual FRW eqs with typical radiation dominated universe. For this situation we obtain dynamics of scale factors for any geometry without any priori assumption. For nonconstant scalar fields and spatially flat geometries, solutions are found to be power law and exponential ones. We also employ weak energy condition for induced matter, that allows negative/positive pressures. All types of solutions fulfill WEC in different ranges. The power law solutions with negative/positive pressures admit both decelerating and accelerating ones. Some solutions accept shrinking extra dimension. By considering nonghost scalar fields and recent observational measurements, solutions are more restricted. We illustrate that accelerating power law solutions, which satisfy WEC and have nonghost fields, are compatible with recent observations in ranges -4/3 < \omega </- -1.3151 and 1.5208 </- n < 1.9583 for dependence of fifth dimension scale factor with usual scale factor. These ranges also fulfill condition nonghost fields in the equivalent 4D vacuum BD eqs.Comment: 18 pages, 16 figures, 11 table

Heatr9 is an infection responsive gene that affects cytokine production in alveolar epithelial cells

During infection, viruses enter susceptible host cells in order to replicate their components for production of new virions. In the process of infection, the gene expression of infected cells undergoes changes because of the production of viral components and due to the host response from detection of viral products. In the advent of RNA sequencing, the discovery of new genes and their functions in the host response generates new avenues for interventions in the host-pathogen interaction. We have identified a novel gene, Heatr9, as a virus and cytokine inducible viral responsive gene. We confirm Heatr9's expression in vitro and in vivo during virus infection and correlate it with viral burden. Heatr9 is induced by influenza virus and RSV. Heatr9 knockdown during viral infection was shown to affect chemokine expression. Our studies identify Heatr9 as a novel inflammatory and virus infection induced gene that can regulate the induction of specific cytokines

Rapid in vitro generation of bona fide exhausted CD8+ T cells is accompanied by Tcf7 promotor methylation

Exhaustion is a dysfunctional state of cytotoxic CD8+ T cells (CTL) observed in chronic infection and cancer. Current in vivo models of CTL exhaustion using chronic viral infections or cancer yield very few exhausted CTL, limiting the analysis that can be done on these cells. Establishing an in vitro system that rapidly induces CTL exhaustion would therefore greatly facilitate the study of this phenotype, identify the truly exhaustion-associated changes and allow the testing of novel approaches to reverse or prevent exhaustion. Here we show that repeat stimulation of purified TCR transgenic OT-I CTL with their specific peptide induces all the functional (reduced cytokine production and polyfunctionality, decreased in vivo expansion capacity) and phenotypic (increased inhibitory receptors expression and transcription factor changes) characteristics of exhaustion. Importantly, in vitro exhausted cells shared the transcriptomic characteristics of the gold standard of exhaustion, CTL from LCMV cl13 infections. Gene expression of both in vitro and in vivo exhausted CTL was distinct from T cells anergy. Using this system, we show that Tcf7 promoter DNA methylation contributes to TCF1 downregulation in exhausted CTL. Thus this novel in vitro system can be used to identify genes and signaling pathways involved in exhaustion and will facilitate the screening of reagents that prevent/reverse CTL exhaustion

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Design for Wire and Arc Additive Layer Manufacture

Additive Layer Manufacture (ALM) is a technique whereby freeform structures are produced by building up material in layers. RUAM (Ready-to-Use Additive Layer Manufacturing) is an innovative concept for building large scale metal ready-to-use parts. The design for RUAM has several process steps: the geometric design of the parts taking the complex process behaviour of the arc welding process into account; FEM to predict temperature and stress distributions to minimise part distortions; and efficient robot tool path design. This paper covers these essential design steps from a technical as well as practical point of view

Sites for priority biodiversity conservation in the Caribbean Islands Biodiversity Hotspot

The Caribbean Islands Biodiversity Hotspot is exceptionally important for global biodiversity conservation due to high levels of species endemism and threat. A total of 755 Caribbean plant and vertebrate species are considered globally threatened, making it one of the top Biodiversity Hotspots in terms of threat levels. In 2009, Key Biodiversity Areas (KBAs) were identified for the Caribbean Islands through a regional-level analysis of accessible data and literature, followed by extensive national-level stakeholder consultation. By applying the Vulnerability criterion, a total of 284 Key Biodiversity Areas were defined and mapped as holding 409 (54%) of the region’s threatened species. Of these, 144 (or 51%) overlapped partially or completely with protected areas. Cockpit Country, followed by Litchfield Mountain - Matheson’s Run, Blue Mountains (all Jamaica) and Massif de la Hotte (Haiti) were found to support exceptionally high numbers of globally threatened taxa, with more than 40 such species at each site. Key Biodiversity Areas, building from Important Bird Areas, provide a valuable framework against which to review the adequacy of existing national protected-area systems and also to prioritize which species and sites require the most urgent conservation attention