International Journal of Pharmaceutical Science and Health Care Issue 5, Vol. 1 (Jan-Feb. 2015) Available online on http://www.rspublication.com/ijphc/index.html ISSN 2249 – 5738

ABSTRACT The present study collects data on Zeta potential (ZP) of erythrocytes of patients suffering from Vaginal Discharge Syndrome (VDS), Syphilis, Herpes, Molluscum and Klebsiella granulomatis (KG). The causative organisms for VDS are Candida species, Gardnerella vaginalis, Trichomonas vaginalis, Neisseria gonorrhoeae and Chlamydia trachomatis. Micro-electrophoresis cell as per Seaman was set up. Approximately 0.01 ml blood was transferred into 50 ml of freshly prepared isotonic dextrose solution. A potential gradient of 150 mV was applied across the electrodes and time required for the erythrocytes to travel a fixed distance of ocular micrometer scale was recorded. ZP of healthy human volunteers was found in the range of 15.28 to 36.66 mV and for these disease patients was found in the range of 2.00 mV to 14.49 mV. Using unpaired t-test it was observed that ZP of disease patients was significantly lower than that of healthy volunteers. The mean ZP of VDS patient was found to be 9. 76, 7.25, 3.35, 4.90 and 8.42 mV for Candidiasis, Bacterial vaginosis, Trichomoniasis, Chlamydia and Gonorrhoea patients respectively. The ZP of the Syphilis, Herpes, Molluscum and KG patients was found to be 3.98, 6.04, 6.23 and 8.07 mV respectively. One-way ANNOVA studies revealed that Trichomoniasis affects the ZP of VDS patients the most followed by syphilis and Chlamydia. It was observed that ZP for Candidiasis was towards normal, indicating it to be the least severe. Therefore, by performing relevant statistical procedures ZP can be used for diagnosis of various disease conditions

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Abstract Studies and a survey were made in Wielki Las Range and also, after year 1995, in the adjacent area. The research is a part of the survey aimed at cataloguing and valorisation of the project named "Ecological-Etnographic Landscape Park in Mogilnica Valley"