Biofilm formation by Rhodococcus equi and putative association with macrolide resistance

Abstract: Rhodococcus equi is a facultative intracellular pathogen, which cause severe pyogranulomatous pneumonia in foals and tuberculosis-like lesions in humans. Its ability to form biofilm was described in strains isolated from chronic diseases associated to treatment failures in humans. This study aimed to verify the biofilm formation by 113 R. equi isolated from equine samples (clinical and fecal) using two different methods (biofilm-culturing with and without additional glucose and epifluorescence microscopy). We also aimed to determine the efficacy of azithromycin, clarithromycin and erythromycin on R. equi in established biofilm. We found 80.5% (26/41) and 63% (58/72) biofilm-positive isolates, in fecal and clinical samples, respectively. The additional glucose increased the biofilm formation by R. equi fecal samples, but not by clinical samples. The antimicrobials tested herein were not able to eradicate R. equi in biofilm even at higher concentrations. This is the first study showing the biofilm formation by R. equi isolated from equine samples. Our findings indicate that R. equi biofilm-producers may be more resistant to the antimicrobials evaluated. Further studies are warranted to test this hypothesis

Models of the strongly lensed quasar DES J0408-5354

We present detailed modelling of the recently discovered, quadruply lensed quasar J0408−5354, with the aim of interpreting its remarkable configuration: besides three quasar images (A,B,D) around the main deflector (G1), a fourth image (C) is significantly reddened and dimmed by a perturber (G2) which is not detected in the Dark Energy Survey imaging data. From lens models incorporating (dust-corrected) flux ratios, we find a perturber Einstein radius 0.04 arcsec ≲ RE, G2 ≲ 0.2 arcsec and enclosed mass Mp(RE, G2) ≲ 1.0 × 10(10) M⊙. The main deflector has stellar mass log10(M⋆/M⊙) = 11.49(+0.46)(−0.32), a projected mass Mp(RE, G1) ≈ 6 × 10(11) M⊙ within its Einstein radius RE, G1 = (1.85 ± 0.15) arcsec and predicted velocity dispersion 267–280 km s(−1). Follow-up images from a companion monitoring campaign show additional components, including a candidate second source at a redshift between the quasar and G1. Models with free perturbers, and dust-corrected and delay-corrected flux ratios, are also explored. The predicted time-delays (ΔtAB = (135.0 ± 12.6) d, ΔtBD = (21.0 ± 3.5) d) roughly agree with those measured, but better imaging is required for proper modelling and comparison. We also discuss some lessons learnt from J0408−5354  on lensed quasar finding strategies, due to its chromaticity and morphology.ISSN:0035-8711ISSN:1365-2966ISSN:1365-871