Relevante mathematische Kompetenzen von Ingenieurstudierenden im ersten Studienjahr - Ergebnisse einer empirischen Untersuchung

Fehlende Kompetenzen in Mathematik und Naturwissenschaften werden von Studierenden als ein Grund für den Studienabbruch in Ingenieurwissenschaften angegeben (Heublein et al., 2017). Welche Kompetenzen für Studierende zu Beginn des Ingenieurstudiums relevant sind, ist jedoch bisher wenig empirisch untersucht. Das Ziel der vorliegenden Studie ist, relevante mathematische Kompetenzen von Ingenieurstudierenden zu analysieren und dabei sowohl Wissensbestände als auch die Anwendung von Wissen und die Zusammenhänge zwischen beiden Bereichen zu berücksichtigen. Dazu wurde eine Studie im Mixed-Methods Design entwickelt. In dieser werden die Studierenden hinsichtlich ihrer Dispositionen in Mathematik und Physik zu Beginn des Studiums und am Ende des ersten Studienjahres mit quantitativen Methoden getestet. Zu diesen beiden und einem weiteren Zeitpunkt am Ende des ersten Semesters wurden zudem die situationsspezifischen Fähigkeiten bei der Bearbeitung von Mathematik- und Physikaufgaben mit Hilfe eines theoretischen Rahmens zum mathematischen Problemlösen mit qualitativen Methoden untersucht. Dieser Theorierahmen umfasste für die Mathematikaufgaben die Aspekte Heurismen (Bruder & Collet, 2011; Schoenfeld, 1980) und Problemlösephasen (Polya, 1957) sowie das Modell der Epistemic Games (Tuminaro, 2004) zur Analyse der Bearbeitung von Physikaufgaben. Die Ergebnisse zeigen Zusammenhänge zwischen mathematischen und physikali-schen Dispositionen. Zusätzlich wird die Bedeutung von Aspekten des Problemlösens deutlich, um die Prozesse bei den Bearbeitungen von Mathematik und Physikaufgaben im ersten Studienjahr zu analysieren. Auf Grundlage der qualitativen Beschreibungen konnten Cluster von Fällen von Studierenden gebildet werden. Mit Hilfe dieser Cluster zeigen sich Zusammenhänge zwischen den Dispositionen und situationsspezifischen Fähigkeiten bei den besonders leistungsstarken und leistungsschwachen Studierenden.Missing competences in mathematics and sciences are cited by students as a reason for the drop-out in engineering sciences (Heublein et al., 2017). However, the competences that are relevant for students at the beginning of their engineering studies have so far not been investigated in an empirical way. The aim of this study is to analyse relevant mathematical competences of engineering students, taking into account both knowledge and the application of knowledge and the interrelationships between the two. A study in mixed method design was developed for this purpose. In this study, students are tested with regard to their dispositions in mathematics and physics at the beginning of their studies and at the end of the first year of their studies using quantitative methods. At these two points in time and a further time at the end of the first semester, the situation-specific skills in processing math and physics tasks were examined with the help of a theoretical framework for solving mathematical problems, using qualitative methods. This theoretical framework included for the mathematical tasks the aspects heuristics (Bruder & Collet, 2011; Schoenfeld, 1980) and problem solving phases (Polya, 1957) as well as the model of Epistemic Games (Tuminaro, 2004) for the analysis of the processing of physical tasks. The results show interrelationships between mathematical and physical dispositions. In addition, it became clear that there is a need of problem solving aspects in order to analyse the processes involved in the working on maths and physics tasks in the first year of studies. Based on the qualitative descriptions, clusters of student cases could be formed. These clusters show the interrelationships between dispositions and situation-specific skills of particularly high-performing and underperforming students

Induction of Bim and Bid gene expression during accelerated apoptosis in severe sepsis

ABSTRACT: INTRODUCTION: In transgenic animal models of sepsis, members of the Bcl-2-family of proteins regulate lymphocyte apoptosis and survival of sepsis. This study investigates the gene regulation of pro- and anti-apoptotic members of the Bcl-2-family of proteins in patients with early stage severe sepsis. METHODS: In this prospective case-control study patients were recruited from three intensive care units in a university hospital. Sixteen patients were enrolled as soon as they fulfilled the criteria of severe sepsis. Ten critically ill but non-septic patients and eleven healthy volunteers served as controls. Blood samples were immediately obtained at inclusion. To confirm the presence of accelerated apoptosis in the patient groups, caspase-3 activation and phosphatidylserine (PS) externalization in CD4+, CD8+ and CD19+ lymphocyte subsets were assessed by flow cytometry. Specific mRNA's of Bcl-2 family members were quantified from whole blood by real-time polymerase chain reaction. To test for statistical significance, Kruskal-Wallis testing with Dunn's multiple comparison test for post hoc testing was performed. RESULTS: In all lymphocyte populations caspase-3 (p<0.05) was activated, which was reflected in an increased PS externalization (p<0.05). Accordingly, lymphocyte counts were decreased in early severe sepsis. In CD4+ T-cells (p<005) and in B-cells (p<0.001) the Bcl-2 protein was decreased in severe sepsis. Gene expression of the BH3-only Bim was massively upregulated as compared to critically ill patients (p<0.001) and 51.6 fold as compared to healthy controls (p<0.05). Bid was increased 12.9 fold compared to critically ill (p<0.001). In the group of the mitochondrial apoptosis-inducers, Bak was upregulated 5.6 fold, while the expression of Bax showed no significant variations. By contrast, the pro-survival members Bcl-2 and Bcl-xl were both downregulated in severe sepsis (p<0.001, p<0.05). CONCLUSIONS: In early severe sepsis a gene expression pattern with induction of the pro-apoptotic Bcl-2 family members Bim, Bid and Bak and a downregulation of the anti-apoptotic Bcl-2 and Bcl-xl was observed in peripheral blood. This constellation may affect cellular susceptibility to apoptosis and complex immune dysfunction in sepsis

A MIF haplotype is associated with the outcome of patients with severe sepsis: a case control study

<p>Abstract</p> <p>Background</p> <p>Macrophage migration inhibitory factor (MIF) plays an important regulatory role in sepsis. In the promoter region a C/G single nucleotide polymorphism (SNP) at position -173 (rs755622) and a CATT_5-8microsatellite at position -794 are related to modified promoter activity. The purpose of the study was to analyze their association with the incidence and outcome of severe sepsis.</p> <p>Methods</p> <p>Genotype distributions and allele frequencies in 169 patients with severe sepsis, 94 healthy blood donors and 183 postoperative patients without signs of infection or inflammation were analyzed by real time PCR and Sequence analysis. All included individuals were Caucasians.</p> <p>Results</p> <p>Genotype distribution and allele frequencies of severe sepsis patients were comparable to both control groups. However, the genotype and allele frequencies of both polymorphisms were associated significantly with the outcome of severe sepsis. The highest risk of dying from severe sepsis was detectable in patients carrying a haplotype with the alleles -173 C and CATT₇(p = 0.0005, fisher exact test, RR = 1,806, CI: 1.337 to 2.439).</p> <p>Conclusion</p> <p>The haplotype with the combination of the -173 C allele and the -794 CATT₇allele may not serve as a marker for susceptibility to sepsis, but may help identify septic patients at risk of dying.</p

Improved detection of blood stream pathogens by real-time PCR in severe sepsis

Objective: Evaluation of the technical and diagnostic feasibility of commercial multiplex real-time polymerase chain reaction (PCR) for detection of blood stream infections in a cohort of intensive care unit (ICU) patients with severe sepsis, performed in addition to conventional blood cultures. Design: Dual-center cohort study. Setting: Surgical ICU of two university hospitals. Patients and participants: One hundred eight critically ill patients fulfilling the American College of Chest Physicians/Society of Critical Care Medicine (ACCP/SCCM) severe sepsis criteria were included. Interventions: None. Measurements and results: PCR results obtained in 453 blood samples from 108 patients were compared with corresponding blood culture results. PCR resulted in a twofold higher positivity rate when compared with conventional blood culture (BC) testing (114 versus 58 positive samples). In 40 out of 58 PCR positive assays the results of the corresponding blood cultures were identical to microorganisms detected by PCR. In 18 samples PCR and BC yielded discrepant results. Compared with conventional blood culture the sensitivity and specificity of PCR was 0.69 and 0.81, respectively. Further evaluation of PCR results against a constructed gold standard including conventional microbiological test results from other significant patient specimen (such as bronchio-alveolar lavage fluid, urine, swabs) and additionally generated clinical and laboratory information yielded sensitivity of 0.83 and specificity of 0.93. Conclusions: Our cohort study demonstrates improved pathogen detection using PCR findings in addition to conventional blood culture testing. PCR testing provides increased sensitivity of blood stream infection. Studies addressing utility including therapeutic decision-making, outcome, and cost-benefit following diagnostic application of PCR tests are needed to further assess its value in the clinical settin

Validating an assessment of pre-service primary teachers’ mathematics pedagogical content knowledge

Mathematikdidaktisches Wissen stellt eine zentrale Komponente der professionellen Kompetenz von (angehenden) Primarstufenlehrkräften im Bereich Mathematik dar, die mit situationsspezifischen Fertigkeiten zusammenhängt und, vermittelt über das professionelle Handeln, Effekte auf die mathematischen Leistungen von Schüler:innen haben kann. Um mathematikdidaktisches Wissen im Zusammenhang mit anderen Komponenten professioneller Kompetenz (z. B. Emotionen, Überzeugungen, Handlungsplanung, Instruktionsqualität) untersuchen zu können, bedarf es eines ökonomischen und frei verfügbaren Tests, welcher gängige Gütekriterien hinreichend erfüllt. Der vorliegende Beitrag stellt die Validierung eines solchen Tests vor. Entsprechend der Teststandards werden Grundnahmen in den Evidenzkategorien Inhalt, Struktur und Beziehungen zu anderen Variablen untersucht, um zu prüfen, ob die Testscores Schlussfolgerungen zum Konstrukt mathematikdidaktisches Wissen von Lehramtsstudierenden der Primarstufe zulassen. Die Ergebnisse liefern Validitätsargumente, die auf eine hinreichend hohe Reliabilität des Tests hinweisen und für theoriekonforme Schlussfolgerungen basierend auf den Testwerten sprechen. Der Beitrag schließt ab mit einer integrativen Betrachtung der Validierungsevidenzen, die für den Test bisher vorliegen.Peer Reviewe

New Applications of Electrochemically Produced Porous Semiconductors and Nanowire Arrays

The growing demand for electro mobility together with advancing concepts for renewable energy as primary power sources requires sophisticated methods of energy storage. In this work, we present a Li ion battery based on Si nanowires, which can be produced reliable and cheaply and which shows superior properties, such as a largely increased capacity and cycle stability. Sophisticated methods based on electrochemical pore etching allow to produce optimized regular arrays of nanowires, which can be stabilized by intrinsic cross-links, which serve to avoid unwanted stiction effects and allow easy processing