9,582 research outputs found
Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
EXPORTERS' MARKET POWER IN AGRICULTURAL IMPORT MARKETS IN KOREA
Korea relies more than 70 percent of food supply on a limited number of exporting counties. If the suppliers possess market power, the food security matters. This study tested whether the major exporting countries of corn and wheat have power of determining market prices and enjoy oligopolistic profit in Korea. The empirical results indicate that Korea shows a high degree of import loyalty toward U.S. products. It is evident that the U.S. and Australia have market power in the wheat market, while no distinct power is detected in the corn market. The result for the corn market seems to reflect the severe price competition between the two major exporters, the U.S. and China. It is also shown that the launch of the WTO does not significantly change the structure of market power in the markets considered.International Relations/Trade,
Institutional learning--the public housing process
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Urban Studies and Planning, 1988.Bibliography: leaves 142-147.by Yang Won Lee.Ph.D
Preparative Synthesis of dTDP-L-Rhamnose Through Combined Enzymatic Pathways
dTDP-L-rhamnose, an important precursor of O-antigen, was prepared on a large scale from dTMP by executing an one-pot reaction in which six enzymes are involved. Two enzymes, dTDP-4-keto-6-deoxy-D-glucose 3,5-epimerase and dTDP-4-keto-rhamnose reductase, responsible for the conversion of dTDP-4-keto-6-deoxy- D-glucose to dTDP-L-rhamnose, were isolated from their putative sequences in the genome of Mesorhizobium loti, functionally expressed in Escherichia coli, and their enzymatic activities were identified. The two enzymes were combined with an enzymatic process for dTDP-4- keto-6-deoxy-D-glucose involving TMP kinase, acetate kinase, dTDP-glucose synthase, and dTDP-glucose 4,6- dehydratase, which allowed us to achieve a preparative scale synthesis of dTDP-L-rhamnose using dTMP and glucose-1-phosphate as starting materials. About 82% yield of dTDP-L-rhamnose was obtained based on initial dTMP concentration at 20 mM dTMP, 1 mM ATP, 10 mM NADH, 60 mM acetyl phosphate, and 80 mM glucose-1- phosphate. From the reaction with 20 ml volume, approximately 180 mg of dTDP-L-rhamnose was obtained in an overall yield of 60% after two-step purification, that is, anion exchange chromatography and gel filtration for desalting. The purified product was identifiedbyHPLC, ESI-MS,andNMR,showingabout95%purity
New time-scale criteria for model simplification of bio-reaction systems
<p>Abstract</p> <p>Background</p> <p>Quasi-steady state approximation (QSSA) based on time-scale analysis is known to be an effective method for simplifying metabolic reaction system, but the conventional analysis becomes time-consuming and tedious when the system is large. Although there are automatic methods, they are based on eigenvalue calculations of the Jacobian matrix and on linear transformations, which have a high computation cost. A more efficient estimation approach is necessary for complex systems.</p> <p>Results</p> <p>This work derived new time-scale factor by focusing on the problem structure. By mathematically reasoning the balancing behavior of fast species, new time-scale criteria were derived with a simple expression that uses the Jacobian matrix directly. The algorithm requires no linear transformation or decomposition of the Jacobian matrix, which has been an essential part for previous automatic time-scaling methods. Furthermore, the proposed scale factor is estimated locally. Therefore, an iterative procedure was also developed to find the possible multiple boundary layers and to derive an appropriate reduced model.</p> <p>Conclusion</p> <p>By successive calculation of the newly derived time-scale criteria, it was possible to detect multiple boundary layers of full ordinary differential equation (ODE) models. Besides, the iterative procedure could derive the appropriate reduced differential algebraic equation (DAE) model with consistent initial values, which was tested with simple examples and a practical example.</p
Characterization of GDP-mannose Pyrophosphorylase from Escherichia Coli O157:H7 EDL933 and Its Broad Substrate Specificity
GDP-mannose pyrophosphorylase gene (ManC) of Escherichia coli (E. coli) O157 was cloned and expressed as a highly soluble protein in E. coli BL21 (DE3). The enzyme was subsequently purified using hydrophobic and ion exchange chromatographies. ManC showed very broad substrate specificities for four nucleotides and various hexose-1-phosphates, yielding ADP-mannose, CDP-mannose, UDP-mannose, GDP-mannose, GDP-glucose and GDP-2-deoxy-glucose
GaIA: Graphical Information Gain based Attention Network for Weakly Supervised Point Cloud Semantic Segmentation
While point cloud semantic segmentation is a significant task in 3D scene
understanding, this task demands a time-consuming process of fully annotating
labels. To address this problem, recent studies adopt a weakly supervised
learning approach under the sparse annotation. Different from the existing
studies, this study aims to reduce the epistemic uncertainty measured by the
entropy for a precise semantic segmentation. We propose the graphical
information gain based attention network called GaIA, which alleviates the
entropy of each point based on the reliable information. The graphical
information gain discriminates the reliable point by employing relative entropy
between target point and its neighborhoods. We further introduce anchor-based
additive angular margin loss, ArcPoint. The ArcPoint optimizes the unlabeled
points containing high entropy towards semantically similar classes of the
labeled points on hypersphere space. Experimental results on S3DIS and
ScanNet-v2 datasets demonstrate our framework outperforms the existing weakly
supervised methods. We have released GaIA at https://github.com/Karel911/GaIA.Comment: WACV 2023 accepted pape
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