One-pot Enzymatic Synthesis of Deoxy-thymidine-diphosphate (TDP)-2-deoxy-∝-d-glucose Using Phosphomannomutase

Production of deoxy-thymidine-diphosphate (TDP)-sugars as substrates of glycosyltransferases, has been one of main hurdles for combinatorial antibiotic biosynthesis, which combines sugar moiety with aglycon of various antibiotics. Here, we report the one-pot enzymatic synthesis of TDP-2-deoxy-glucose employing high efficient TMP kinase (TMK; E.C. 2.7.2.12), acetate kinase (ACK; E.C. 2.7.1.21), and TDP-glucose synthase (TGS; E.C. 2.7.7.24) with phosphomannomutase (PMM; E.C. 5.4.2.8). In this study, replacing phosphoglucomutase (PGM; E.C. 5.4.2) by PMM from Escherichia coli gave four times higher specific activity on 2-deoxy-6-phosphate glucose, suggesting that the activity on 2-deoxy-glucose-6-phosphate was mainly affected by PMM activity, not PGM activity. Using an in vitro system starting from TMP and 2-deoxy-glucose-6-phosphate glucose, TDP-2-deoxy-glucose (63% yield) was successfully synthesized. Considering low productivity of NDP-sugars from cheap starting materials, this paper showed how production of NDP-sugars could be enhanced by controlling mutase activity

Cornell Potential Parameters for S-wave Heavy Quarkonia

We compute derived quantities for various values of the model parameter of the Cornell potential model for the S-wave heavy quarkonia with radial quantum numbers n=1, 2, and 3. Our results can be used to determine leading and relative-order-v^2 nonrelativistic quantum chromodynamics matrix elements for S-wave charmonia and bottomonia such as psi(2S), eta_c(2S), and Upsilon(nS) for n=1, 2, and 3. These matrix elements will be essential ingredients for resumming relativistic corrections to processes involving those S-wave heavy quarkonium states.Comment: 10 pages, 2 figures, 1 table, version to be published in J. Korean Phys. So