Real-time Image Processing for Microscopy-based Label-free Imaging Flow Cytometry in a Microfluidic Chip

Imaging flow cytometry (IFC) is an emerging technology that acquires single-cell images at high-throughput for analysis of a cell population. Rich information that comes from high sensitivity and spatial resolution of a single-cell microscopic image is beneficial for single-cell analysis in various biological applications. In this paper, we present a fast image-processing pipeline (R-MOD: Real-time Moving Object Detector) based on deep learning for high-throughput microscopy-based label-free IFC in a microfluidic chip. The R-MOD pipeline acquires all single-cell images of cells in flow, and identifies the acquired images as a real-time process with minimum hardware that consists of a microscope and a high-speed camera. Experiments show that R-MOD has the fast and reliable accuracy (500 fps and 93.3% mAP), and is expected to be used as a powerful tool for biomedical and clinical applications.113Ysciescopu

Deep Reinforcement Learning for Chatbots Using Clustered Actions and Human-Likeness Rewards

Training chatbots using the reinforcement learning paradigm is challenging due to high-dimensional states, infinite action spaces and the difficulty in specifying the reward function. We address such problems using clustered actions instead of infinite actions, and a simple but promising reward function based on human-likeness scores derived from human-human dialogue data. We train Deep Reinforcement Learning (DRL) agents using chitchat data in raw text—without any manual annotations. Experimental results using different splits of training data report the following. First, that our agents learn reasonable policies in the environments they get familiarised with, but their performance drops substantially when they are exposed to a test set of unseen dialogues. Second, that the choice of sentence embedding size between 100 and 300 dimensions is not significantly different on test data. Third, that our proposed human-likeness rewards are reasonable for training chatbots as long as they use lengthy dialogue histories of ≥10 sentences