The Vertical Vorticity Structure within a Squall Line Observed during BAMEX: Banded Vorticity Features and the Evolution of a Bowing Segment

Abstract A quasi-linear convective line with a trailing stratiform region developed during the Bow Echo and Mesoscale Convective Vortex Experiment (BAMEX) while being sampled by two airborne Doppler radars. The finescale reflectivity and Doppler velocities recorded by the radars documented the evolution of the convective line. Bands of positive and negative vertical vorticity oriented parallel to the convective line were resolved in the analysis. This type of structure has rarely been reported in the literature and appears to be a result of the tilting and subsequent stretching of ambient horizontal vorticity produced by the low-level wind shear vector with a significant along-line component. The radar analysis also documented the evolution of an embedded bow echo within the convective line. Embedded bow echoes have been documented for a number of years; however, a detailed analysis of their kinematic structure has not been previously reported in the literature. The counterrotating circulation patterns that are characteristic of bow echoes appeared to be a result of tilting and stretching of the horizontal vorticity produced in the cold pool. The analysis suggests that the location along the convective line where embedded bow echoes form depends on the local depth of the cold pool. The rear-inflow jet is largely driven by the combined effects of the counterrotating vortices and the upshear-tilted updraft

An axisymmetric view of concentric eyewall evolution in Hurricane Rita (2005)

J. Atmos. Sci., 69 2414-2432The article of record as published may be located at http://dx.doi.org/10.1175/JAS-D-11-0167.

Aqueous Extract from Hibiscus sabdariffa Linnaeus Ameliorate Diabetic Nephropathy via Regulating Oxidative Status and Akt/Bad/14-3-3γ in an Experimental Animal Model

Several studies point out that oxidative stress maybe a major culprit in diabetic nephropathy. Aqueous extract of Hibiscus sabdariffa L. (HSE) has been demonstrated as having beneficial effects on anti-oxidation and lipid-lowering in experimental studies. This study aimed at investigating the effects of Hibiscus sabdariffa L. on diabetic nephropathy in streptozotocin induced type 1 diabetic rats. Our results show that HSE is capable of reducing lipid peroxidation, increasing catalase and glutathione activities significantly in diabetic kidney, and decreasing the plasma levels of triglyceride, low-density lipoprotein (LDL) and increasing high-density lipoprotein (HDL) value. In histological examination, HSE improves hyperglycemia-caused osmotic diuresis in renal proximal convoluted tubules (defined as hydropic change) in diabetic rats. The study also reveals that up-regulation of Akt/Bad/14-3-3γ and NF-κB-mediated transcription might be involved. In conclusion, our results show that HSE possesses the potential effects to ameliorate diabetic nephropathy via improving oxidative status and regulating Akt/Bad/14-3-3γ signaling

Shorter GT repeat polymorphism in the heme oxygenase-1 gene promoter has protective effect on ischemic stroke in dyslipidemia patients

<p>Abstract</p> <p>Background</p> <p>The microsatellite polymorphism of heme oxygenase (HO)-1 gene promoter has been shown to be associated with the susceptibility to ischemic event, including coronary artery disease (CAD), myocardial infarction, and peripheral vascular disease. We aimed to examine whether the length of (GT)_nrepeats in HO-1 gene promoter is associated with ischemic stroke in people with CAD risk factors, especially low level of HDL.</p> <p>Methods</p> <p>A total of 183 consecutive firstever ischemic stroke inpatients and 164 non-stroke patients were screened for the length of (GT)_nrepeats in HO-1 promoter. The long (L) and short (S) genotype are defined as the averaged repeat number >26 and ≦26, respectively.</p> <p>Results</p> <p>Stroke patients tended to have more proportions of hypertension, diabetics and genotype L, than those of genotype S. Patients with genotype L of HO-1 gene promoter have higher stroke risk in comparison with genotype S especially in dyslipidemia individuals. The significant differences on stroke risk in multivariate odds ratios were found especially in people with low HDL-C levels.</p> <p>Conclusions</p> <p>Subjects carrying longer (GT)_nrepeats in HO-1 gene promoter may have greater susceptibility to develop cerebral ischemic only in the presence of low HDL-C, suggesting the protective effects in HO-1 genotype S in the process of ischemic stroke, particularly in subjects with poor HDL-C status.</p

Rapamycin attenuates PLA2R activation-mediated podocyte apoptosis via the PI3K/AKT/mTOR pathway

Membranous nephropathy (MN) is the most common cause of nephrotic syndrome in adults without diabetes. Primary MN has been associated with circulating antibodies against native podocyte antigens, including phospholipase A2 receptor (PLA2R); however, precision therapy targeting the signaling cascade of PLA2R activation is lacking. Both PLA2R and the mammalian target of rapamycin (mTOR) exist in podocytes, but the interplay between these two proteins and their roles in MN warrants further exploration. This study aimed to investigate the crosstalk between PLA2R activation and mTOR signaling in a human podocyte cell line. We demonstrated that podocyte apoptosis was induced by Group IB secretory phospholipase A2 (sPLA2IB) in a concentration- and time-dependent manner via upregulation of phosphoinositide 3-kinase (PI3K), protein kinase B (AKT), and mTOR, and inhibited by rapamycin or LY294002. Furthermore, aberrant activation of the PI3K/AKT/mTOR pathway triggers both extrinsic (caspase-8 and caspase-3) and intrinsic (Bcl-2-associated X protein [BAX], B-cell lymphoma 2 [BCL-2], cytochrome c, caspase-9, and caspase-3) apoptotic cascades in podocytes. The therapeutic implications of our findings are that strategies to reduce PLA2R activation and PI3K/AKT/mTOR pathway inhibition in PLA2R-activated podocytes help protect podocytes from apoptosis. The therapeutic potential of rapamycin shown in this study provides cellular evidence supporting the repurposing of rapamycin for MN treatment

Effects of HMGB-1 Overexpression on Cell-Cycle Progression in MCF-7 Cells

High mobility group-1 (HMGB-1) enhances the DNA interactions and possesses a transcriptional activation potential for several families of sequence-specific transcriptional activators. In order to examine the effect of HMGB-1 on the cell cycle progression in MCF-7 cells, the HMGB-1 expression vector was transfected into synchronized MCF-7 cells, and the effect of HMGB-1 overexpression on the cell cycle was examined. The HMGB-1 protein level in the transfected cells increased 4.87-fold compared to the non-transfected cells. There were few changes in the cell cycle phase distribution after HMGB-1 overexpression in the MCF-7 cells. Following the estrogen treatment, the cell cycle progressed in both the HMGB-1 overexpressed MCF-7 and the mock-treated cells. However, a larger proportion of HMGB-1 overexpressing MCF-7 cells progressed to the either S or G2 phase than the mock-treated cells. The mRNA levels of the cell cycle regulators changed after being treated with estrogen in both the HMGB-1 overexpressing MCF-7 and the mock-treated cells, but the changes in the expression level of the cell cycle regulator genes were more prominent in the HMGB-1 overexpressing MCF-7 cells than in the mock-treated cells. In conclusion, HMGB-1 overexpression itself does not alter the MCF-7 cell cycle progression, but the addition of estrogen to the HMGB-1 overexpressing MCF-7 cells appears to accelerate the cell cycle progression

Near-Surface Vortex Structure in a Tornado and in a Sub-Tornado-Strength Convective-Storm Vortex Observed by a Mobile, W-Band Radar during VORTEX2

Abstract As part of the Second Verification of the Origins of Rotation in Tornadoes Experiment (VORTEX2) field campaign, a very high-resolution, mobile, W-band Doppler radar collected near-surface (≤200 m AGL) observations in an EF-0 tornado near Tribune, Kansas, on 25 May 2010 and in sub-tornado-strength vortices near Prospect Valley, Colorado, on 26 May 2010. In the Tribune case, the tornado's condensation funnel dissipated and then reformed after a 3-min gap. In the Prospect Valley case, no condensation funnel was observed, but evidence from the highest-resolution radars in the VORTEX2 fleet indicates multiple, sub-tornado-strength vortices near the surface, some with weak-echo holes accompanying Doppler velocity couplets. Using high-resolution Doppler radar data, the authors document the full life cycle of sub-tornado-strength vortex beneath a convective storm that previously produced tornadoes. The kinematic evolution of these vortices, from genesis to decay, is investigated via ground-based velocity track display (GBVTD) analysis of the W-band velocity data. It is found that the azimuthal velocities in the Tribune tornado fluctuated in concert with the (dis)appearance of the condensation funnel. However, the dynamic pressure drop associated with the retrieved azimuthal winds was not sufficient to account for the condensation funnel. In the Prospect Valley case, the strongest and longest-lived sub-tornado-strength vortex exhibited similar azimuthal velocity structure to the Tribune tornado, but had weaker azimuthal winds. In both cases, the radius of maximum azimuthal wind was inversely related to the wind speed, and changes in the axisymmetric azimuthal component of velocity were consistent with independent indicators of vortex intensification and decay

Evolutionary Scheduler for the Deep Space Network

A computer program assists human schedulers in satisfying, to the maximum extent possible, competing demands from multiple spacecraft missions for utilization of the transmitting/receiving Earth stations of NASA s Deep Space Network. The program embodies a concept of optimal scheduling to attain multiple objectives in the presence of multiple constraints

What are the most effective behaviour change techniques to promote physical activity and/or reduce sedentary behaviour in inactive adults? : A systematic review protocol

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ The version of record, Howlett N, Trivedi D, Troop NA, et al, 'What are the most effective behaviour change techniques to promote physical activity and/or reduce sedentary behaviour in inactive adults? A systematic review protocol', BMJ Open, Vol 5(8), 2015; 5:e008573, is available online via doi: 10.1136/bmjopen-2015-008573 Published by MBJ Publishing Group Limited.Large proportions of the population are not meeting recommended levels of physical activity and have increasingly sedentary lifestyles. Low levels of physical activity are predictive of poor health outcomes and time spent sedentary is related to a host of risk factors independently of physical activity levels. Building an evidence base of the best approaches to intervene in the lifestyles of inactive individuals is crucial in preventing long-term disease, disability and higher mortality ratesPeer reviewedFinal Published versio