Cellular stress characterisation in S. cerevisiae budding yeast using optical microscopy

The term "cellular stress" covers various environmental and metabolic events that threaten cell survival. In response, eukaryotic cells can adapt their metabolism to acute changes in their surrounding environment to maintain cellular homeostasis and ensure survival. The cell is an enclosed system with macromolecules and functional compartments diffusing in a liquid-like environment, the cytoplasm. The position in time and space of these elements influences every aspect of cell biology, from molecular interactions and enzymatic activities to the process of cell division itself. Cellular stress episodes can interfere with the timing of these physiological processes: perturbations modify the cytoplasm volume and composition (e.g., accumulation of damaged proteins), including changes of intracellular physical properties such as macromolecular crowding influencing diffusion and spatio-temporal dynamics of the whole system. Consequently, cellular stress dynamics have been of strong interest to physicists and biologists. In continuity with previous research in the field, this project aimed to explore these aspects of cellular physiology and gain new insight into cellular stress responses and crowding dynamics in the budding yeast Saccharomyces cerevisiae, a eukaryotic model sensitive to environmental stresses. This thesis sets out to investigate the influence of hyperosmotic shock, glucose availability, and cell growth on macromolecular crowding. I present a methodology developed to identify local regions of crowding in yeast cells using a previously generated Förster Resonance Energy Transfer Technology (FRET) crowding biosensor called CrGE. I describe experimental and analysis procedures to quantify crowding at subcellular levels and have developed new strains controlling the expression of fluorescently tagged cytoplasmic aggregates. To identify fluorescent clusters on cellular models, single molecule characterisations for stoichiometry and diffusion tracks in vivo were performed using bespoke Slimfield microscopy. Cellular sub-compartments were visualised and tracked over time using confocal microscopy, giving insight into polarised inheritance events in the budding yeast

Application of bag sampling technique for particle size distribution measurements

Bag sampling techniques can be used to temporarily store an aerosol and therefore provide sufficient time to utilize sensitive but slow instrumental techniques for recording detailed particle size distributions. Laboratory based assessment of the method were conducted to examine size dependant deposition loss coefficients for aerosols held in VelostatTM bags conforming to a horizontal cylindrical geometry. Deposition losses of NaCl particles in the range of 10 nm to 160 nm were analysed in relation to the bag size, storage time, and sampling flow rate. Results of this study suggest that the bag sampling method is most useful for moderately short sampling periods of about 5 minutes

The second flight of the Sunrise Balloon-borne Solar Observatory: overview of instrument updates, the flight, the data, and first results

S. K. Solanki et. al.©2017 The American Astronomical Society. All rights reserved.The Sunrise balloon-borne solar observatory, consisting of a 1 m aperture telescope that provides a stabilized image to a UV filter imager and an imaging vector polarimeter, carried out its second science flight in 2013 June. It provided observations of parts of active regions at high spatial resolution, including the first high-resolution images in the Mg ii k line. The obtained data are of very high quality, with the best UV images reaching the diffraction limit of the telescope at 3000 Å after Multi-Frame Blind Deconvolution reconstruction accounting for phase-diversity information. Here a brief update is given of the instruments and the data reduction techniques, which includes an inversion of the polarimetric data. Mainly those aspects that evolved compared with the first flight are described. A tabular overview of the observations is given. In addition, an example time series of a part of the emerging active region NOAA AR 11768 observed relatively close to disk center is described and discussed in some detail. The observations cover the pores in the trailing polarity of the active region, as well as the polarity inversion line where flux emergence was ongoing and a small flare-like brightening occurred in the course of the time series. The pores are found to contain magnetic field strengths ranging up to 2500 G, and while large pores are clearly darker and cooler than the quiet Sun in all layers of the photosphere, the temperature and brightness of small pores approach or even exceed those of the quiet Sun in the upper photosphere.The German contribution to Sunrise and its reflight was funded by the Max Planck Foundation, the Strategic Innovations Fund of the President of the Max Planck Society (MPG), DLR, and private donations by supporting members of the Max Planck Society, which is gratefully acknowledged. The Spanish contribution was funded by the Ministerio de Economía y Competitividad under Projects ESP2013-47349-C6 and ESP2014-56169-C6, partially using European FEDER funds. The National Center for Atmospheric Research is sponsored by the National Science Foundation. The HAO contribution was partly funded through NASA grant number NNX13AE95G. This work was partly supported by the BK21 plus program through the National Research Foundation (NRF) funded by the Ministry of Education of Korea. L.G. acknowledges research funding from the State of Lower Saxony, Germany. SDO is a mission of NASA's Living With a Star (LWS) program. The SDO/HMI data were provided by the Joint Science Operation Center (JSOC). The National Solar Observatory (NSO) is operated by the Association of Universities for Research in Astronomy (AURA) Inc. under a cooperative agreement with the National Science Foundation.Peer reviewe

Physico-chemical characterization of walnut shell biochar from uncontrolled pyrolysis in a garden oven and surface modification by ex-situ chemical magnetization

The shells of walnuts (WS) are major refuse in the global fruits and nuts trade. This, otherwise discarded, lignin-rich material can be carbonized to biochar—a value-added product with environmental applications such as carbon sequestration, soil amelioration, and pollutant adsorption. These applications are dictated by structural and chemical characteristics of the biochar carbon. Conventional controlled pyrolysis (CPy) of biomass is cost-intensive and technically too complex for widespread adoption, especially in emerging economies. Here, walnut shell biochar (BWS0) is derived through uncontrolled pyrolysis (UCPy) in a pyrolysis oven and further hybridized as magnetic biochar through ex-situ chemical co-precipitation. The physico-chemical characteristics of biochar and its water-extractable fractions are comprehensively investigated to understand their carbon structure and environmental applicability. The sp2 amorphous carbon sequestered in BWS0 is 0.84 kgCO2/kgbiomass with a BET (N2) surface area of 292 m2/g and is comparable to biochar from CPy in terms of carbon structure. The polyaromatic hydrocarbons present are only trace amounts of naphthalene, biphenyl, and phenanthrene. The magnetization decreases porosity of BWS0 while greatly facilitating its separation from aqueous media. BWS0 is suitable for adsorption of cations (between pH 2.8 and 9.45) and hydrophobic pollutants with only 19 mg L−1 fouling from their intrinsic dissolved organic carbon. In combination with fast-release N, P fertilizers, BWS0 (C/N of 24.8) is suitable for application in hydrophilic soils at higher loading rates. The results suggest an avenue where WS biochar can also be prepared via UCPy for direct environmental applications. Future investigations into soil incubation and adsorption tests are recommended. Graphical abstract: [Figure not available: see fulltext.

WindCube: A CubeSat Thermospheric Wind Instrument Utilizing Fabry-Pérot Interferometry

WindCube is a 6U CubeSat mission selected for implementation through NASA’s HFORT program. Starting in 2022 and following ~36 months of development, integration and testing, the spacecraft will operate for ~12 months in low earth orbit to study the influence of thermospheric winds on the earth’s ionosphere. Its scientific payload is a limb viewing Fabry Pérot Interferometer (étalon) specially designed to fit into a 10cm x 10cm cross-sectional assembly. WindCube will make global maps of wind speed derived from the doppler shifted emission of the 630.0nm oxygen line (1D \u3e 3P), at altitudes near 250km. Projected performance includes wind speed retrievals every 10 seconds with an accuracy of 5m/s, a vertical resolution of 63km, and a horizontal resolution of 100km. We present an overview of the mission design and observation plan for WindCube as well as a top-level description of the payload design

Biochar synthesis from mineral and ash-rich waste biomass, part 2: characterization of biochar and co-pyrolysis mechanism for carbon sequestration

The increase in mineral and ash-rich waste biomass (MWB) generation in emerging economies poses critical environmental problems and bottlenecks the solid waste and wastewater treatment systems. Transforming these MWB such as sewage sludge from wastewater treatment (SSW) to biochar can be a sustainable method for their disposal and resource recovery. However, such biochar has limited applicability due to the relatively low organic content and possibly contaminated nature of SSW. This may be offset through combined pyrolysis with other MWB, which can also support municipal solid waste management. Studies on this MWB co-pyrolysis are lacking and have not yet seen successful long-term implementation. This work is the second part of authors’ research encompassing an analytical and lab-scale investigation of biochar production from MWB through pyrolysis for the case of Chennai city, India. Here, the physicochemical properties of biochar derived from lab-scale co-pyrolysis of SSW with other MWB such as anaerobic digestate from waste to energy plants of food, kitchen or market waste fermentation, and banana peduncles (BP) collected from vegetable markets and their thermolysis mechanism are comprehensively investigated for purpose of carbon sequestration. Also, a novel preliminary investigation of the effect of sample weight (scaling effect) on the analytical pyrolysis of biomass (BP as model substrate) is undertaken to elucidate its impact on the heat of pyrolysis and carbon distribution in resultant biochar. The maximum carbon sequestration potential of the derived biochar types is 0.22 kg CO2 kg−1 biomass. The co-pyrolysis of MWB is exothermic and governed by the synergetic effects of the components in blends with emission profiles following the order CO2 > CH4 > CO > NH3. Co-pyrolysis reduced the heavy metal enrichment in SSW biochar. The derived biochars can be an immediate source of N, P and S in nutrient-deficient acidic soils. The biochar has only up to 4-ring polyaromatic compounds and a residence time longer than 1 h at 500 °C is necessary to improve carbonization. The heat released during analytical pyrolysis of the model biomass and distribution of carbon in the resultant biochar are significantly influenced by scaling effects, drawing attention to the need for a more detailed scaling investigation of biomass pyrolysis

The second flight of the SUNRISE balloon-borne solar observatory: overview of instrument updates, the flight, the data and first results

The SUNRISE balloon-borne solar observatory, consisting of a 1~m aperture telescope that provided a stabilized image to a UV filter imager and an imaging vector polarimeter, carried out its second science flight in June 2013. It provided observations of parts of active regions at high spatial resolution, including the first high-resolution images in the Mg~{\sc ii}~k line. The obtained data are of very high quality, with the best UV images reaching the diffraction limit of the telescope at 3000~\AA\ after Multi-Frame Blind Deconvolution reconstruction accounting for phase-diversity information. Here a brief update is given of the instruments and the data reduction techniques, which includes an inversion of the polarimetric data. Mainly those aspects that evolved compared with the first flight are described. A tabular overview of the observations is given. In addition, an example time series of a part of the emerging active region NOAA AR~11768 observed relatively close to disk centre is described and discussed in some detail. The observations cover the pores in the trailing polarity of the active region, as well as the polarity inversion line where flux emergence was ongoing and a small flare-like brightening occurred in the course of the time series. The pores are found to contain magnetic field strengths ranging up to 2500~G and, while large pores are clearly darker and cooler than the quiet Sun in all layers of the photosphere, the temperature and brightness of small pores approach or even exceed those of the quiet Sun in the upper photosphere.Comment: Accepted for publication in The Astrophysical Journa

Solar Site Survey for the Advanced Technology Solar Telecope. I. Analysis of the Seeing Data

The site survey for the Advanced Technology Solar Telescope concluded recently after more than two years of data gathering and analysis. Six locations, including lake, island and continental sites, were thoroughly probed for image quality and sky brightness. The present paper describes the analysis methodology employed to determine the height stratification of the atmospheric turbulence. This information is crucial because day-time seeing is often very different between the actual telescope aperture (~30 m) and the ground. Two independent inversion codes have been developed to analyze simultaneously data from a scintillometer array and a solar differential image monitor. We show here the results of applying them to a sample subset of data from May 2003, which was used for testing. Both codes retrieve a similar seeing stratification through the height range of interest. A quantitative comparison between our analysis procedure and actual in situ measurements confirms the validity of the inversions. The sample data presented in this paper reveal a qualitatively different behavior for the lake sites (dominated by high-altitude seeing) and the rest (dominated by near-ground turbulence).Comment: To appear in the Publications of the Astronomical Society of the Pacific (PASP). Note: Figures are low resolution versions due to file size limitation

The Sunrise Mission

The first science flight of the balloon-borne \Sunrise telescope took place in June 2009 from ESRANGE (near Kiruna/Sweden) to Somerset Island in northern Canada. We describe the scientific aims and mission concept of the project and give an overview and a description of the various hardware components: the 1-m main telescope with its postfocus science instruments (the UV filter imager SuFI and the imaging vector magnetograph IMaX) and support instruments (image stabilizing and light distribution system ISLiD and correlating wavefront sensor CWS), the optomechanical support structure and the instrument mounting concept, the gondola structure and the power, pointing, and telemetry systems, and the general electronics architecture. We also explain the optimization of the structural and thermal design of the complete payload. The preparations for the science flight are described, including AIV and ground calibration of the instruments. The course of events during the science flight is outlined, up to the recovery activities. Finally, the in-flight performance of the instrumentation is briefly summarized.Comment: 35 pages, 17 figure