Surf zone hazards and injuries on beaches in SW France

Surf zone injuries (SZIs) are common worldwide, yet limited data is available for many geographical regions, including Europe. This study provides the first preliminary overview of SZIs along approximately 230 km of hazardous surf beaches in SW France during the summer season. A total of 2523 SZIs over 186 sample days during the summers of 2007, 2009 and 2015 were analysed. Documented injury data included date and time; beach location; flag colour; outside/inside of the bathing zone; age, gender, country and home postal code of the victim; activity; cause of injury; injury type and severity. Injuries sustained ranged from mild contusion to fatal drowning, including severe spinal injuries, wounds and luxation. While the most severe injuries (drowning) were related to rip currents, a large number of SZIs occurred as a result of shore-break waves (44.6%; n = 1125) and surfing activity (31.0%; n = 783) primarily inside and outside of lifeguard patrolled bathing zones, respectively. Victims were primarily French living more than 40 km from the beach (75.9% of the reported addresses; n = 1729), although a substantial number of victims originated from Europe (14.7% of the addresses reported; n = 335), including The Netherlands (44.2%; n = 148), Germany (26.3%; n = 88) and Belgium (12.5%; n = 49). The predominant age group involved in the incidents was between 10-25 years (54.5%; n = 1376) followed by 35-50 years (22.6%; n = 570), with the majority of SZIs involving males (69.6%, n = 1617). Despite the large predominance (74.1%; n = 33) of males involved in the most severe drowning incidents, all of which occurred outside the bathing zone, a surprisingly large proportion of females (48.0%; n = 133) experienced milder drowning incidents involving only minor to moderate respiratory impairment, peaking at 58.2% (n = 85) within the age group 10-25. The spine/cervical injury population is very young, with 58.5% (n = 313) within the age group 10-20. Specific injuries tended to occur in clusters (e.g. rip current drowning or shore-break injury) with particular days prone to rip-current drowning or hazardous shore-break waves, suggesting the potential to predict level of risk to beachgoers based on basic weather and marine conditions. This study calls for increased social-based beach safety research in France and the development of more effective public awareness campaigns to highlight the surf zone hazards, even within a supervised bathing zone. These campaigns should be targeted towards young males and females, in order to reduce the number of injuries and drownings occurring on beaches in SW France.Marier les objectifs de défense côtière avec ceux de la protection du milieu naturel grâce aux dunes sableuse

Benzo[a]pyrene, Aflatoxine B1 and Acetaldehyde Mutational Patterns in TP53 Gene Using a Functional Assay: Relevance to Human Cancer Aetiology

Mutations in the TP53 gene are the most common alterations in human tumours. TP53 mutational patterns have sometimes been linked to carcinogen exposure. In hepatocellular carcinoma, a specific G>T transversion on codon 249 is classically described as a fingerprint of aflatoxin B1 exposure. Likewise G>T transversions in codons 157 and 158 have been related to tobacco exposure in human lung cancers. However, controversies remain about the interpretation of TP53 mutational pattern in tumours as the fingerprint of genotoxin exposure. By using a functional assay, the Functional Analysis of Separated Alleles in Yeast (FASAY), the present study depicts the mutational pattern of TP53 in normal human fibroblasts after in vitro exposure to well-known carcinogens: benzo[a]pyrene, aflatoxin B1 and acetaldehyde. These in vitro patterns of mutations were then compared to those found in human tumours by using the IARC database of TP53 mutations. The results show that the TP53 mutational patterns found in human tumours can be only partly ascribed to genotoxin exposure. A complex interplay between the functional impact of the mutations on p53 phenotype and the cancer natural history may affect these patterns. However, our results strongly support that genotoxins exposure plays a major role in the aetiology of the considered cancers

Mutagenicity of Nitrosubstituted and Amino-substituted Carbazoles In Salmonella-typhimurium .1. Monosubstituted Derivatives of 9h-carbazole

Mononitro, monoamino and monoacetamido carbazoles were assayed for mutagenicity in Salmonella typhimurium strains TA1535, TA1538, TA1537, TA1977, TA98 and TA100, with and without the addition of S9 from phenobarbital-induced rat liver. The role of bacterial metabolism of the nitro group was also studied using the additional strains TA98NR and TA98/1, 8DNP6. None of the compounds was active in TA1535, while only 2-nitrocarbazole and 3-nitrocarbazole presented a weak mutagenicity towards its pKM101 derivative, TA100. All four nitrocarbazole isomers were mutagenic for TA1538 and TA98, their activities decreasing in the order: 2-nitrocarbazole almost-equal-to 3-nitrocarbazole > 1-nitrocarbazole > 4-nitrocarbazole. Direct-acting mutagenicities for TA1537 were lower than for TA1538, but varied in the same order. Nitro reduction was an important step of metabolic activation of nitrocarbazoles, as indicated by the dramatic reduction of activity with TA98NR, as compared to TA98. Results obtained with TA98/1,8DNP6 showed that O-acetyltransferase was only partly required for the expression of mutagenic potency of these compounds. 2-Aminocarbazole was a weak direct-acting mutagen for TA1538 and TA98. Its activity was strongly increased in the presence of S9 mix, while 3-aminocarbazole became active in these conditions. The acetamido derivatives were consistently less mutagenic than their parent amines. These results show that nitrocarbazoles and aminocarbazoles behave as reactive frameshift mutagens, acting mainly through the formation of esterified hydroxylamines. The very low activity of 4-nitrocarbazole might be related to an orientation of the nitro group perpendicular to the aromatic ring