Plasmonic DNA: Towards Genetic Diagnosis Chips

"Plasmonics and DNA" special issueInternational audienc

Multidimension Potential Of Surface Plasmon Resonance Imaging For Dynamic Surface Characterization: Application To Optical Biochips System

We have realized a surface plasmon resonance imaging system allowing accurate characterization of biochips. In this paper, the Rouard approach is extended to absorbing layers to model the reflectivity information contained in the multidimensional data. ©2005 Optical Society of America

Multidimension Potential Of Surface Plasmon Resonance Imaging For Dynamic Surface Characterization: Application To Optical Biochips

We have realized a surface plasmon resonance imaging system allowing accurate characterization of biochips. In this paper, the Rouard approach is extended to absorbing layers to model the reflectivity information contained in the multidimensional data. The multidimension potential is also expressed to demonstrate the power of the SPR imaging system. To conclude, towards the development of a biosensor based on SPR, a theoretical study is also performed on the sensitivity to changes in reflectivity of such multidimension optical biosensor. The sensitivity of the sytem shows the power of this biophotonic technology. © 2005 SPIE-OSA

Metallic Film Optimization In A Surface Plasmon Resonance Biosensor By The Extended Rouard Method

The extended Rouard method is applied to the computation of a multi-absorbing-layer system for the optimization of surface plasmon resonance (SPR) sensors. Specifically, the effect of the properties of a metallic layer on the shape of the reflectivity and sensitivity curve is demonstrated in the case of a Kretschmann configuration. This theoretical investigation allows us to establish the best optical properties of the metal to obtain a localized SPR, given the illuminating beam properties. Toward the development of a sensitive biosensor based on SPR, we quantify the changes in reflectivity of such an optical biosensor induced by the deposition of a nanometric biochemical film as a function of the metal film characteristics and the illumination operating conditions. The sensitivity of the system emphasizes the potential of such biophotonic technology using metallic multilayer configurations, especially with envisioned metamaterials. © 2007 Optical Society of America

Generalization Of The Rouard Method To An Absorbing Thin-Film Stack And Application To Surface Plasmon Resonance

In the context of surface plasmon resonance (SPR) kinetic biochips, it is important to model the SPR phenomenon (i.e., extinction of reflectivity) toward biochip design and optimization. The Rouard approach that models reflectivity off a thin-film stack is shown to be extendable to any number of absorbing layers with no added complexity. Using the generalized Rouard method, the effect of SPR is simulated as a function of the wavelength for various metal thicknesses. Given an optimal metal thickness, the dependence of SPR on the angle of incidence and wavelength is also demonstrated. Such a model constitutes a potential basis for the efficient design and optimization of multidimensional sensors. © 2006 Optical Society of America

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International audienceSensors based on surface plasmon resonance have the potential to provide information on the binding of biological molecules on adequate substrates over typically thousand channels in parallel, without the need for any marker and in real time compared to the scale of biochemical reactions. The need to optimize selectivity and sensitivity has triggered continued research efforts. We review those related with optics and image processing, at the same time identifying some aspects that deserve further investigation before the potential of the technique is fully utilized

Surface plasmon resonance imaging as a multidimensional surface characterization instrument - application to biochip genotyping

International audienc

Multispectral imaging of a biochip based surface plasmon resonance

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Surface plasmon resonance imaging (SPRI) system and real-time monitoring of DNA biochip for human genetic mutation diagnosis of DNA amplified samples

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