Standardization of a Technique For Obtaining DNA From Footprints

Currently our country has high numbers of missing persons, Tamaulipas being one of the states with the highest rate of disappearances. The identification of people has become more important thanks to the development of molecular techniques. However, the limitations are very high, because it is necessary to compare the genetic pattern of the disappeared with the parents. Therefore, the objective of this research is to standardize a genomic DNA extraction technique from contact surfaces for its subsequent implementation in the identification of disappeared, allowing the comparison of the genetic pattern with the disappeared itself. For this, genomic DNA extraction was carried out using the Phenol-Chloroform technique from fingerprint samples on a slide. The analysis was performed in duplicate on 5 fingerprint donors at various times; 24 hours, 7 days, 15 days, 30 days and the quality and concentration of DNA was obtained by means of a Nanodrop spectrophotometer. An increase in DNA concentration was shown during the exposure time but a decrease in quality without presenting statistically significant differences (p\u3e 0.05). These results may be because with the passing of days and exposure to the environment it interferes with the quality of DNA due to the presence of nucleases

Resultados Semilleros de Investigación 2009-2010

La publicación recoge los doce informes finales de investigación presentados por los estudiantes de ocho Semilleros 1 y cuatro Semilleros 2, correspondientes a la convocatoria 2009–2010 y se constituye en el Número 25 de la Serie de Investigaciones en Construcción, si bien este es el primer Número publicado en formato digital que UNIJUS se permite poner a disposición no sólo de la comunidad universitaria, sino también de la sociedad colombiana e internacional, interesada en los temas estudiados por los jóvenes investigadores de la Facultad de Derecho, Ciencias Políticas y Sociales de la Universidad Nacional de Colombia