23 research outputs found

    Etude des substances allelochimiques impliquees dans la relation tournesol-pyrale du tournesol (Homoeosoma nebullelum Denis et Schiffermuller)

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    194 ref. * INRA, Laboratoire de Neurobiologie Comparee des Invertebres, Bures sur Yvette, (FRA) Diffusion du document : INRA, Laboratoire de Neurobiologie Comparee des Invertebres, Bures sur Yvette, (FRA) Diplôme : Dr. d'Universit

    Quantitative and qualitative analysis of rapeseed flower nectar (Brassica napus L. var oleifera Metzer).

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    La secretion nectarifere des fleurs de cybrides males-steriles de colza d'hiver (Brassica napus L)

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    National audienceFloral nectar secretions of 5 male sterile cybrids of winter rapeseed (25, 58, 77, 85, 118) and the Darmor variety were compared after pipette collection and analysis of their sugar composition using high pressure liquid chromatography. The influence of various parameters was studied. Regarding the effect of time of collection (fig 1, table I): the nectar secretions at 8 am were more abundant (mean of 60%) and less concentrated (≈ 1/3) than those at 2 pm; the relative concentrations of each sugar (glucose, fructose) were similar at 8 am and 2 pm and close to 50%. Compared to Darmor, cybrids produced less nectar (50% less for 27, 58, 85 and 90% less for 77 and 118). Regarding the effect of the day of collection (fig 2, table II): on average, the secretions were more abundant on May 3rd than on April 15th (23% more). Independently of the day, Darmor secreted more than the cybrids (65-95% more). The dry matter (19.3-22.4 mg/100 μl) and the mean concentrations of each sugar (≈ 50%) were similar every day. Regarding the combined effect of the day and location (fig 3, tables III, IV): for every day and location, secretions were similar within cybrids and lower than that in Darmor (54-58% less). Amounts secreted (2-4 μl on average) and total sugar amounts (≈ 15-40 mg/100 μl) differed according to day and location, whereas the relative concentrations of each sugar remained constant. Thus, although large fluctuations might occur in the amounts of nectar, the glucidic composition of the nectars appeared to remain constant in each cybrid and favorable for entomophilous pollination.Les sécrétions nectarifères des fleurs de 5 cybrides (27, 58, 77, 85 et 118) mâles-stériles de colza d’hiver (Brassica napus L) et de la variété Darmor mâle-fertile, ont été comparées après une analyse de leur composition glucidique par chromatographie liquide à haute pression. Par rapport à Darmor, les cybrides ont sécrété moins de nectar (50% de moins pour 27, 58 et 85, et 90% de moins pour 77 et 118), mais les concentrations de leurs constituants glucidiques n’ont pas varié de façon significative. Selon les lieux, les dates et l’heure du prélèvement, seules les quantités de nectar recueillies et les concentrations en matière sèche ont varié. Les concentrations en glucose et fructose sont restées voisines de 50% sans variation significative; le saccharose a été peu ou pas représenté. Ainsi, bien que d’importantes variations de quantités sécrétées puissent survenir, la composition glucidique des nectars des cybrides apparaît stable et satisfaisante dans la perspective d’une pollinisation entomophile
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