45 research outputs found
Chicago Music City
Chicago Music City compares the strength and vitality of music industries and scenes across the United States. Sociologists, urban planners, and real-estate developers point to quality of life and availability of cultural amenities as important indicators of the health and future success of urban areas. Economic impact studies show the importance of music to local economies. This publication compares Chicago's musical strength with the 50 largest metropolitan areas in the U.S., focusing on 11 comparison cities: Chicago and its demographic peers, New York and Los Angeles, and eight other cities with strong musical reputations -- Atlanta, Austin, Boston, Las Vegas, Memphis, Nashville, New Orleans and Seattle
Mapping State Cultural Policy: The State of Washington
State-level funding for the arts, humanities, heritage, and allied forms of culture is an important source of financial support, dwarfing the aid provided by the National Endowment for the Arts. This investigation, underwritten by the Pew Charitable Trusts, shows that states support culture through policies and programs scattered across state government and through means that go beyond direct funding
Role Of MinD-Membrane Association in Min Protein Interactions
Division site placement in Escherichia coli involves interactions of the MinD protein with MinC and MinE and with other MinD molecules to form membrane-associated polymeric structures. In this work, as part of a study of these interactions, we established that heterologous membrane-associated proteins such as MinD can be targeted to the yeast nuclear membrane, dependent only on the presence of a membrane-binding domain and a nuclear targeting sequence. Targeting to the nuclear membrane was equally effective using the intrinsic MinD membrane-targeting domain or the completely unrelated membrane-targeting domain of cytochrome b(5). The chimeric proteins differing in their membrane-targeting sequences were then used to establish the roles of membrane association and specificity of the membrane anchor in MinD interactions, using the yeast two-hybrid system. The chimeric proteins were also used to show that the membrane association of MinD and MinE in E. coli cells had no specificity for the membrane anchor, whereas formation of MinDE polar zones and MinE rings required the presence of the native MinD membrane-targeting sequence
Mapping the MinE Site Involved in Interaction with the MinD Division Site Selection Protein of Escherichia coli
Interactions between the MinD and MinE proteins are required for proper placement of the Escherichia coli division septum. The site within MinE that is required for interaction with MinD was mapped by studying the effects of site-directed minE mutations on MinD-MinE interactions in yeast two-hybrid and three-hybrid experiments. This confirmed that the MinE N-terminal domain is responsible for the interaction of MinE with MinD. Mutations that interfered with the interaction defined an extended surface on one face of the α-helical region of the MinE N-terminal domain, consistent with the idea that the MinE-MinD interaction involves formation of a coiled-coil structure by interaction with a complementary helical surface within MinD
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Chicago Music City
Chicago Music City, a first-of-its kind study conducted by the Cultural Policy Center at the University of Chicago, compares the strength and vitality of music industries and scenes across the United States, and finds that Chicago is a leader by nearly each indicator measured. As cities across the United States vie with each other to attract and retain business, sociologists, urban planners, and real estate developers point to quality of life and availability of cultural amenities as important indicators of the health and future success of urban areas. A number of these cities are turning to economic impact studies to show the importance of the music to the local economies. Chicago Music City compares Chicago’s musical strength with the 50 largest metropolitan areas in the U. S., focusing especially on a group of eleven comparison cities: Chicago and its demographic peers, New York and Los Angeles, plus eight others with strong musical reputations – Atlanta, Austin, Boston, Las Vegas, Memphis, Nashville, New Orleans and Seattle. Initially intended to serve as a benchmark for measuring the future growth of the Chicago’s music community, Chicago Music City offers a new twist on the economic impact studies used by music industry and arts advocates across the country