Determination of sensitivity paypel- biopsy method compared to medical diagnostic curettage

The data comparison cohort prospective study, the sensitivity of the paypel - biopsy method against medical diagnostic curettage for the diagnosis of endometrial pathology in 120 aged 46-55 years women. Endometrial hyperplasia by paypel - biopsy method in 47% (56) patients, versus 65% (79) (95% Cl [38%; 73%]) in patients after medical diagnostic curettage was found. The sensitivity of the paypel - biopsy method was 70,8% (p = 0,04).Представлены данные сравнительного когортного проспективного исследования эффективности методов пайпель-биопсии и лечебно-диагностического выскабливания для диагностики патологии эндометрия у 120 женщин в возрасте 46-55 лет. Гиперплазия эндометрия методом пайпель-биопсии была выявлена у47% (56) пациенток, против 65% (79) (95%ДИ [38%; 73%]) пациенток после лечебно-диагностического выскабливания. Чувствительность метода пайпель-биопсии составила 70,8% (р=0.04)

Analyses of carnivore microsatellites and their intimate association with tRNA-derived SINEs

BACKGROUND: The popularity of microsatellites has greatly increased in the last decade on account of their many applications. However, little is currently understood about the factors that influence their genesis and distribution among and within species genomes. In this work, we analyzed carnivore microsatellite clones from GenBank to study their association with interspersed repeats and elucidate the role of the latter in microsatellite genesis and distribution. RESULTS: We constructed a comprehensive carnivore microsatellite database comprising 1236 clones from GenBank. Thirty-three species of 11 out of 12 carnivore families were represented, although two distantly related species, the domestic dog and cat, were clearly overrepresented. Of these clones, 330 contained tRNA(Lys)-derived SINEs and 357 contained other interspersed repeats. Our rough estimates of tRNA SINE copies per haploid genome were much higher than published ones. Our results also revealed a distinct juxtaposition of AG and A-rich repeats and tRNA(Lys)-derived SINEs suggesting their coevolution. Both microsatellites arose repeatedly in two regions of the insterspersed repeat. Moreover, microsatellites associated with tRNA(Lys)-derived SINEs showed the highest complexity and less potential instability. CONCLUSION: Our results suggest that tRNA(Lys)-derived SINEs are a significant source for microsatellite generation in carnivores, especially for AG and A-rich repeat motifs. These observations indicate two modes of microsatellite generation: the expansion and variation of pre-existing tandem repeats and the conversion of sequences with high cryptic simplicity into a repeat array; mechanisms which are not specific to tRNA(Lys)-derived SINEs. Microsatellite and interspersed repeat coevolution could also explain different distribution of repeat types among and within species genomes. Finally, due to their higher complexity and lower potential informative content of microsatellites associated with tRNA(Lys)-derived SINEs, we recommend avoiding their use as genetic markers