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Not AvailableCastor being a perennial, cross-pollinated, sexually polymorphic crop with high environmental sensitivity, initial plant breeding efforts were restricted to plant height and duration. Domestication of a wild, perennial crop to an annual crop of medium plant height and duration is the first success. Further, development of a two-line breeding system and standard seed production technology led to successful commercial exploitation of heterosis. Being a monotypic genus, diversification of parental base is restricted to intra-generic, intraspecific, or inter-varietal hybridization. Phenotypic expression is highly plastic and varies with locations and seasons. Majority of the morphological characters are monogenic, independently assorted with very limited linkages among the traits. However, information on genetics of major morphological characters is scattered in several old publications. Conventional breeding methods were successful in developing about 40 high-yielding hybrids and varieties with inbuilt resistance to major pests and diseases. An effort is made in the present chapter to consolidate the information on genetics and breeding methods followed in India and elsewhere.Not Availabl

Synovial fluid proteome in rheumatoid arthritis

BACKGROUND: Rheumatoid arthritis (RA) is a chronic autoinflammatory disorder that affects small joints. Despite intense efforts, there are currently no definitive markers for early diagnosis of RA and for monitoring the progression of this disease, though some of the markers like anti CCP antibodies and anti vimentin antibodies are promising. We sought to catalogue the proteins present in the synovial fluid of patients with RA. It was done with the aim of identifying newer biomarkers, if any, that might prove promising in future. METHODS: To enrich the low abundance proteins, we undertook two approaches—multiple affinity removal system (MARS14) to deplete some of the most abundant proteins and lectin affinity chromatography for enrichment of glycoproteins. The peptides were analyzed by LC–MS/MS on a high resolution Fourier transform mass spectrometer. RESULTS: This effort was the first total profiling of the synovial fluid proteome in RA that led to identification of 956 proteins. From the list, we identified a number of functionally significant proteins including vascular cell adhesion molecule-1, S100 proteins, AXL receptor protein tyrosine kinase, macrophage colony stimulating factor (M-CSF), programmed cell death ligand 2 (PDCD1LG2), TNF receptor 2, (TNFRSF1B) and many novel proteins including hyaluronan-binding protein 2, semaphorin 4A (SEMA4D) and osteoclast stimulating factor 1. Overall, our findings illustrate the complex and dynamic nature of RA in which multiple pathways seems to be participating actively. CONCLUSIONS: The use of high resolution mass spectrometry thus, enabled identification of proteins which might be critical to the progression of RA. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12014-016-9113-1) contains supplementary material, which is available to authorized users