A murine herpesvirus closely related to ubiquitous human herpesviruses causes T-cell depletion

ABSTRACT The human roseoloviruses human herpesvirus 6A (HHV-6A), HHV-6B, and HHV-7 comprise the Roseolovirus genus of the human Betaherpesvirinae subfamily. Infections with these viruses have been implicated in many diseases; however, it has been challenging to establish infections with roseoloviruses as direct drivers of pathology, because they are nearly ubiquitous and display species-specific tropism. Furthermore, controlled study of infection has been hampered by the lack of experimental models, and until now, a mouse roseolovirus has not been identified. Herein we describe a virus that causes severe thymic necrosis in neonatal mice, characterized by a loss of CD4 + T cells. These phenotypes resemble those caused by the previously described mouse thymic virus (MTV), a putative herpesvirus that has not been molecularly characterized. By next-generation sequencing of infected tissue homogenates, we assembled a contiguous 174-kb genome sequence containing 128 unique predicted open reading frames (ORFs), many of which were most closely related to herpesvirus genes. Moreover, the structure of the virus genome and phylogenetic analysis of multiple genes strongly suggested that this virus is a betaherpesvirus more closely related to the roseoloviruses, HHV-6A, HHV-6B, and HHV-7, than to another murine betaherpesvirus, mouse cytomegalovirus (MCMV). As such, we have named this virus murine roseolovirus (MRV) because these data strongly suggest that MRV is a mouse homolog of HHV-6A, HHV-6B, and HHV-7. IMPORTANCE Herein we describe the complete genome sequence of a novel murine herpesvirus. By sequence and phylogenetic analyses, we show that it is a betaherpesvirus most closely related to the roseoloviruses, human herpesviruses 6A, 6B, and 7. These data combined with physiological similarities with human roseoloviruses collectively suggest that this virus is a murine roseolovirus (MRV), the first definitively described rodent roseolovirus, to our knowledge. Many biological and clinical ramifications of roseolovirus infection in humans have been hypothesized, but studies showing definitive causative relationships between infection and disease susceptibility are lacking. Here we show that MRV infects the thymus and causes T-cell depletion, suggesting that other roseoloviruses may have similar properties. </jats:p

Viral MHCI inhibition evades tissue-resident memory T cell formation and responses

Tissue-resident memory CD

A Methodology of measurements for phtalocyanine gas sensors dedicated to the detection fo ozone and nitrogen dioxide. Results in urban air pollution context.

International audienc

Metal-n-InP Rectifying Properties Enhancement with Zn Based Metallizations and Diffusion at Moderate Annealing Temperatures

Rectifying contacts on n-InP using Zn based metallizations followed by moderate annealing temperature and time were studied. Diffusion of Zn atoms at the metal-semiconductor interface creates a thin p-InP layer. Pseudo-Schottky junctions were obtained with a significant barrier height enhancement, typically 0.2–0.25 eV. The metallization process involved throughout the present work leads to high quality Schottky diodes within a rather simple procedure similar to this generally used to obtain good ohmic contacts. It is shown in particular that the special requirements needed for a lot of electrical measurements ($e.g.$ $C{-}V$ characteristics or D.L.T.S.) can be matched without any extra complication. The behaviour of Schottky devices was throroughly analysed as a function of the annealing procedure. Best performances were obtained by applying cumulative annealing sequences, increasing the temperature while decreasing the time of exposure. The homogeneity of the structures was attested from a satisfactory agreement between barrier heights deduced either from current or from capacitance measurements. A good linearity of $C^{-2}{-}V{-}T$ curves and low values of the series resistances were also obtained

Carbon Nanotubes-based Gas Sensors for pollutants: Elaboration methods for NO2 and BTX detection.

International audienc

Isang positibong pananaw at paghahanap ng kahulugan sa buhay tambay

Ang isang tambay ay negatibo para sa mga Pilipino dahil sila ay ang mga taong walang trabaho o hindi nag-aaral. Madalas na inuugnay sila sa mga negatibong aktibidad tulad ng paninigarilyo, pag-inom, paggamit ng pinagbabawal na gamot, at iba pa. Ang pag-aaral na ito ay nagbigay pokus sa mga tambay na may gulang labingwalong taon hanggang dalawampu’t lima kung saan sinasabing ito ang estado ng paghubog sa kamalayan ng isang indibidwal particular sa kabataan. Ang mga datos ay nakalap sa pamamagitan ng pakikipagkwentuhan sa tatlong grupo ng mga lalaking tambay. Ang mga nakalap na datos ay sinuri upang makabuo ng mga tema upang makatulong sa pag-aaral. Napagkasunduan ng mga mananaliksik ang mga tema at kategorya sa pag-aanalisa ng mga datos. Ang mga resulta ay naglalarawan sa dahilan, pananaw, aktibidad at epekto ng pagtambay sa mga kabataan. Ayon din sa pag-aaral, ang pagtambay ay hindi lubusang negatibo ay mayroong mga makukuhang positibo ngunit hindi ito hinihikayat ng mga mananaliksik

Functionalized Carbon Nanotubes-Based Gas Sensors for Pollutants Detection: Investigation on the Use of a Double Transduction Mode

International audienc

Noncovalent functionalisation of carbon nanotubes: elaboration of gas sensor dedicated to BTX.

International audienc