Effects of Citrus essential oils on weed emergence and on soil microorganisms

Weed invasion is a major problem for agricultural productivity since causes economic and environmental damages. Weed control can be achieved through chemical, mechanical, biological and cultural means. Although synthetic herbicides are effective for weed control, they have a negative impact on soil and the environment. Therefore, it is important to develop alternative means that are in the meanwhile effective and ecofriendly. EOs are commonly used for bactericidal, virucidal, fungicidal, antiparasitical, insecticidal, medicinal and cosmetic applications. Citrus EOs are the most widely used in the world for many purposes. Moreover, some studies have recently investigated their potential as herbicides. EOs can be extracted in different ways among which hydro distillation and cold pressing are commonly used. The two methods are based on different procedures. Hydro distillation is carried out with a Clevenger-apparatus that conducts the distillation process by boiling, condensing and decantation to separate the EOs. The cold pressing consist of crushing and pressing the peels thus leading to the formation of a watery emulsion. Then, the emulsion is centrifuged to separate out the EOs. Since no external substance are needed, this process ensures that the resulting EOs retains all their properties. The aim of this work was to evaluate the in vivo potential effects of Citrus EOs extracted by hydro distillation and cold pressing on weed emergence and on soil biochemical properties. The topsoil (5 cm) of an Inceptisol within the campus of University of Palermo was used for the experiment. 500 g of soil, air-dried and sieved at 1 cm, were used to fill each of the 24 aluminium pots (10 x 20 cm). then, soils were brought up to 100% of their water holding capacity (WHC) by adding firstly 150 mL of tap water (2/3 of WHC) followed by 70 mL of tap water (1/3 of WHC), by a anual sprayer, containing 8 mL L-1 of each one of the extracted EOs. Then, the soils were maintained at 50% of their water holding capacity during all the period of the experiment. Fitoil was used as emulsifier at a concentration of 1 ml L-1. Soils without EO, and with or without Fitoil, were considered as control. After one month the soils were moistened, plant biomass and height, and soil chemical and biochemical properties were evaluated. The experiment was carried out in quadruplicate to investigate the soil proprieties. In this work, the results are showed and discussed

Improvement of physical, chemical and biochemical proprieties of a salt affected Alfisol by addition of biochar and gypsum

Salinization is one of the major environmental problems threatening agricultural productivity. Soil salinization is defined as an excessive accumulation of salts within the soil profile. It negatively affects soil physical and chemical properties, as well as the biochemical ones. Reclamation of salt affected soils requires removal of soluble salts and Na+ from the soil exchange sites. Subsequently, salts are leached out the root zone by irrigation water when available. Gypsum (CaSO4·2H2O) is the most commonly used chemical amendment for reclamation of salt affected soils since it provides Ca2+ that replaces Na+ on the exchange sites and improves soil structure. Also organic amendments have been considered, but not extensively studied, for reclamation of salt affected soils. Recent studies have reported that biochar can be rich in nutrients like Ca2+ and Mg2+ and may enhance their availability in soil when added as amendment. Therefore, addition of biochar to a salt affected soil could aid in its remediation by supplying Ca2+ and Mg2+, and replacing Na+, improving aggregate stability and hydraulic conductivity. The objective of this study was to evaluate the effects of gypsum and biochar for the reclamation of a saline-sodic soil on some physical, chemical and biological properties. The topsoil of an Alfisol, about fifty meters far from the foreshore in the Petrosino coast (Sicily, Italy), was used for this experiment. The soil was air-dried and sieved at 2 mm. The main physical and chemical properties of the soil were: pH 7.3, clay 23 %, total carbonates 50.9 %, electrical conductivity 0.81 dS m-1 (1:5, w/v), total organic C 11.0 g kg-1, cation exchange capacity 24.8 cmol(+) kg-1, exchangeable sodium percentage 35 %. Two doses of gypsum (2.6 and 5.1 g kg-1 of soil) and two doses of biochar (4.2 and 8.3 g kg-1) were tested. The two doses of gypsum were calculated in order to decrease ESP from 35% to 25% and to 15%, respectively, whereas biochar was added in order to achieve an amount of 10 and 20 Mg ha-1. Following addition of gypsum and biochar, either alone or in combination, 100 g of soil were incubated at room temperature in 150 mL plastic pots and maintained at 50% of soil water holding capacity during all the duration of the experiment (22 days). One week after the incubation, three horse-radish seeds were sown. Then, after 13 days, plants were removed, oven dried at 60°C for 48 hours and weighed. The soils were analyzed to determine porosity, CEC, ESP, ECe, microbial biomass C, soil respiration and microbial community structure. The experiment was carried out in octuplicate. In this work, the results are reported and discusse

Mapping quantitative trait loci affecting biochemical and morphological fruit properties in eggplant (Solanum melongena L.)

Eggplant berries are a source of health-promoting metabolites including antioxidant and nutraceutical compounds, mainly anthocyanins and chlorogenic acid; however, they also contain some anti-nutritional compounds such as steroidal glycoalkaloids (SGA) and saponins, which are responsible for the bitter taste of the flesh and with potential toxic effects on humans. Up to now, Quantitative Trait Loci (QTL) for the metabolic content are far from being characterized in eggplant, thus hampering the application of breeding programs aimed at improving its fruit quality. Here we report on the identification of some QTL for the fruit metabolic content in an F2 intraspecific mapping population of 156 individuals, obtained by crossing the eggplant breeding lines ‘305E40’ x ‘67/3’. The same population was previously employed for the development of a RAD-tag based linkage map and the identification of QTL associated to morphological and physiological traits. The mapping population was biochemically characterized for both fruit basic qualitative data, like dry matter, °Brix, sugars and organic acids, as well as for health-related compounds such chlorogenic acid, (the main flesh monomeric phenol), the two peel anthocyanins (i.e. delphinidin-3-rutinoside (D3R) and delphinidin-3-(p-coumaroylrutinoside)-5-glucoside (nasunin)) and the two main steroidal glycoalkaloids, solasonine and solamargine. For most of the traits, one major QTL (PVE ≥ 10%) was spotted and putative orthologies with other Solanaceae crops are discussed. The present results supply valuable information to eggplant breeders on the inheritance of key fruit quality traits, thus providing potential tools to assist future breeding programs

Evaporation from soils of different texture covered by layers of water repellent and wettable soils

Water repellent soils are able to channel water deep into the soil profile by fingered flow, minimising water storage in the water repellent top layer where water is most susceptible to evaporation. To date, the effect of water repellent or wettable surface layer on evaporation from wet sublayer has only been reported for coarse materials, and an increase in water repellency led to a greater delay in water evaporation. The objective of this study was to assess the effect of water repellent vs. wettable top layers with different thickness on water evaporation from coarse and fine texture subsoils that were pre-moistened. Clay loam soil samples were taken from Pinus pinaster woodland of Ciavolo, Italy, and sandy soil samples from Pinus sylvestris woodland of Sekule, Slovakia. Evaporation from soil samples was determined from the loss of weight in laboratory conditions. Water in the clay loam soil from Ciavolo was held for a longer period due to slower evaporative loss than in the sandy soil from Sekule, and the impact of the water repellent layer on the loss rate over time is related to its thickness. Over 550 hours, about 90% of the initial stored water was evaporated from the uncovered clay-loam soil sample from Ciavolo. In the same time, the 0.3, 1, and 2 cm-thick duff layers, respectively, saved about 23, 34, and 58 % of water from evaporation, and evaporation of 90% of water took over 780, 1100, and 1450 hours. It means that the clay loam soil cover with the 0.3, 1, and 2 cm-thick duff layers resulted in prolonging the evaporation by 10, 23, and 37.5 days, respectively. As to the sandy soil from Sekule, 98% of water was evaporated from the uncovered soil sample over 240 hours. In the same time, the 0.3, 1, and 2 cm-thick water repellent soil layers, respectively, saved about 7, 45, and 59 % of water from evaporation, and evaporation of 98% of water took over 330, 606, and 774 hours. It means that the sandy soil cover with the 0.3, 1, and 2 cm-thick water repellent soil layers resulted in prolonging the evaporation by about 4, 15, and 22 days, respectively. It can be concluded that water repellent surface layers, created by pine trees, are able to delay evaporation significantly for both coarse and fine textured soils, which may be particularly beneficial for plants during hot and dry periods in summer

Source of calcium for pedogenic CaCO3 formation in benchmark soils of a Mediterranean semiarid environment

Calcic and petrocalcic horizons (Bk and Bkm, respectively) commonly occur in soils of arid and semiarid regions. Studies carried out to investigate the source of calcium in CaCO3 formation have shown that dust and parent materials represent the main sources. The aim of this study was to investigate if gypsum may be a source of calcium for calcium carbonate formation and, eventually, to quantify it. Five soil pedons, developing on “Serie Gessoso- Solfifera”, were selected in a very representative forest Mediterranean ecosystem in Sicily (I). Pedons were opened, described in field and sampled according to the sequence of genetic horizons. Soil samples were analyzed to investigate 87/86Sr ratio in leached and residual soil fractions as well as total amount of carbonates and gypsum. Results allow us to assert that gypsum may be an important source of calcium for pedogenic CaCO3 formation in the investigated soils. The amount of calcium arising from gypsum change not only among the pedons but also among the horizons of the same pedon depending on its chemical and physical features

Differenziazione e quantificazione dei carbonati litogenici e pedogenici di Gypsisuoli forestali attraverso l'analisi isotopica

Gli autori riferiscono sui tipi e sui modelli di genesi dei carbonati dei suoli, attraverso l’uso degli isotopi del 13C. Lo studio è stato condotto in un’area dell’interno collinare argilloso siciliano, su suoli che evolvono sui depositi evaporitici della Serie Gessoso-Solfifera. Sono stati scelti cinque pedon su litotipi calcarei e non. I pedon sono stati descritti in campo e campionati secondo la sequenza degli orizzonti genetici; sui campioni di suolo sono state eseguite le seguenti analisi: tessitura, reazione, conducibilità elettrica, gesso, carbonio organico ed inorganico. Inoltre, parallelamente sono state eseguite le analisi isotopiche del carbonio organico ed inorganico. I risultati hanno consentito di individuare, oltre che le complesse relazioni esistenti tra la componente abiotica e biotica del suolo, anche i modelli di genesi dell’orizzonte calcico e quindi le relative implicazioni sulla classificazione dei pedon investigati

Application of ICP-MS for measuring soil metal cations from sequential extraction

The present work is part of a collaboration between Palermo and Seville Universities to investigate the cations incorporated into calcite and its effect in the trace metal mobility in soils. The study area is located in the central part of Sicily. Calcite is usually greatly dispersed and has a major influence on the pH of soils and therefore on trace element behaviour. A great affinity for carbonates has been observed for a wide range of cations incorporated into calcite. in order to investigate the metal cations in soil carbonate, 1M MgCl2 was used to remove the exchangeable Ca. Sub-samples were treated with acid acetic 1M to quantify the total amount of Ca and metal cations in soil carbonate. And finally others sub-samples were used to perform a kinetic extraction with Na-citrate 0.2M. The ICP-MS was selected as a suitable technique to reliable and simultaneously measure trace elements reducing matrix effects. the equipment used was a X7 SERIES ICP_MS from ThermoElemental (UK). the present work shows in detail the methodology used to determine, in acid acetic and Na-citrate solutions, the following isotopes: Cr, Mn, Fe, Co, Ni, Sr, Ba, Pb and U. We have adapted the US EPA method 6020 for our special needs. it is important to remark that a careful procedure has been carry out to reproduce the sample matrix in all the calibration and QC standards, and so properly select the internal standards since the complexity of the matrix

Soil profile dismantlement by land levelling and deep tillage damages soil functioning but not quality

We investigated the effects of land levelling followed by deep tillage, thus inducing a drastic dismantlement of soil profile, on both soil functioning and quality by monitoring various bioindicators (microbial biomass and community structure, basal respiration, enzyme activities) expressed on either whole soil and TOC mass units, respectively. As expected, in disturbed soils all measured properties had much higher coefficients of variation (CVs), regardless of either whole soil or TOC mass basis, due to the induced spatial variability. The amount of total organic C in the first cubic meter of soil profile was of one order of magnitude greater in undisturbed soils compared to disturbed ones. Soil bioindicators monitored on whole soil mass basis appeared greatly worsened while unchanged or even improved under TOC mass basis. This was essentially due to a couple of reasons: (1) soil bioindicators are constitutively dependent on soil TOC content; (2) soil spatial rearrangement may have made some organic C available to microbial biomass, otherwise unreachable when allocated throughout the undisturbed soil profile. Concluding, our work highlighted the need of expressing soil biondicators on soil mass basis when the response of the whole agrosystem to soil deep spatial rearrangement has to be assessed, while on TOC mass basis if the soil biological features are the major concerns

Chemical, biochemical and microbial diversity through a Pachic Humudept profile in a temperate upland grassland.

There is great interest in understanding the factors that drive soil microbial activity and community composition in upland grassland ecosystems. We investigated the role of vertical gradients of chemical properties and various soluble C and N pools on soil microbial community structure by using a combination of chemical and biochemical methods coupled with PCR-denaturing gradient gel electrophoresis (DGGE) community fingerprinting and phospholipid fatty acid (PLFA) profiling. Soil samples were collected at increasing depth from a temperate upland grassland. Soil organic matter-related pools (total organic C and total Kjeldahl N) and functionally related active pools (microbial biomass C and N, K2SO4-extractable C and N, extractable organic N) markedly decreased with soil depth and were positively related to each other. The microbial community deep in the soil profile appeared neither C- nor N-limited. Conversely, DGGE community fingerprinting of bacteria, α-Proteobacteria, β-Proteobacteria and Actinobacteria revealed that each soil horizon was conducive to the dominance of particular ribotypes thus confirming links between soluble nutrients gradients and community structure. Soil microbial biomass C assessed by PLFA content decreased with soil depth less steeply than when estimated by the chloroform fumigation-extraction method. This suggests that chloroform fumigation efficiency in lysing microbial cells varied with soil depth with the varying amount and distribution of total and soluble C. The PLFA biomarker for fungal biomass markedly decreased throughout the three upper horizons, whereas that for arbuscular mycorrhizae strongly decreased in the deeper AB horizon only. Taken together with the increase across soil profile of total saturated-to-total monounsaturated fatty acids ratio, the PLFA data suggest that a compositional shift from fungal to bacterial dominance has taken place throughout the grassland layers