Limiting current in a relativistic diode under the condition of magnetic insulation

The maximum emission current density is calculated for a time-independent, relativistic, cycloidal electron flow in a diode that is under the condition of magnetic insulation. Contrary to conventional thinking, this maximum current is not determined by the space charge limited condition on the cathode, even when the emission velocity of the electrons is assumed to be zero. The self electric and magnetic fields associated with the cycloidal flow are completely accounted for. This maximum current density is confirmed by a two-dimensional, fully electromagnetic and fully relativistic particle-in-cell code. © 2003 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/71144/2/PHPAEN-10-11-4489-1.pd

Projection ablation lithography cathode for high-current, relativistic magnetron

Initial results are presented of an innovative cathode operating in a relativistic magnetron powered by an accelerator with parameters: −0.3 MV−0.3MV, 1–10 kA1–10kA, and 0.5 μs0.5μs pulse length. This cathode is fabricated by ablating a pattern on the cathode using a KrF laser. This projection ablation lithography (PAL) cathode has demonstrated fast current turn-on and microwave startup times have decreased from an average of 193 to 118 ns193to118ns. The pulselength of 1 GHz1GHz microwave oscillation has increased from a 144 ns144ns average to 217 ns217ns. With these improvements in microwave startup and pulse length, the microwave power has approximately remained the same compared to the previously used cloth cathodes. A new triple-azimuthal emission region is tested as means of prebunching the electrons (“cathode priming”) into the three spokes desired for pi mode operation in a six-cavity magnetron. The Tri-PAL cathode priming results in the fastest startup and highest efficiency of relativistic magnetron microwave generation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70745/2/RSINAK-75-9-2976-1.pd

Radio-frequency plasma cleaning for mitigation of high-power microwave-pulse shortening in a coaxial gyrotron

Results are reported demonstrating that radio-frequency (rf) plasma cleaning is an effective technique for mitigating microwave-pulse shortening (i.e., lengthening the pulse) in a multimegawatt, large-orbit, coaxial gyrotron. Cleaning plasmas were generated by 50 W of rf power at 13.56 MHz in nitrogen fill gas in the pressure range 15–25 mTorr. Improvements in the averaged microwave energy output of this high-power-microwave device ranged from 15% to 245% for different initial conditions and cleaning protocols. The mechanism for this improvement is believed to be rf plasma sputtering of excess water vapor from the cavity/waveguide and subsequent removal of the contaminant by cryogenic vacuum pumps. © 2000 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/71003/2/APPLAB-77-23-3725-1.pd

Unveiling the dynamic infrared sky with Gattini-IR

While optical and radio transient surveys have enjoyed a renaissance over the past decade, the dynamic infrared sky remains virtually unexplored. The infrared is a powerful tool for probing transient events in dusty regions that have high optical extinction, and for detecting the coolest of stars that are bright only at these wavelengths. The fundamental roadblocks in studying the infrared time-domain have been the overwhelmingly bright sky background (250 times brighter than optical) and the narrow field-of-view of infrared cameras (largest is 0.6 sq deg). To begin to address these challenges and open a new observational window in the infrared, we present Palomar Gattini-IR: a 25 sq degree, 300mm aperture, infrared telescope at Palomar Observatory that surveys the entire accessible sky (20,000 sq deg) to a depth of 16.4 AB mag (J band, 1.25μm) every night. Palomar Gattini-IR is wider in area than every existing infrared camera by more than a factor of 40 and is able to survey large areas of sky multiple times. We anticipate the potential for otherwise infeasible discoveries, including, for example, the elusive electromagnetic counterparts to gravitational wave detections. With dedicated hardware in hand, and a F/1.44 telescope available commercially and cost-effectively, Palomar Gattini-IR will be on-sky in early 2017 and will survey the entire accessible sky every night for two years. We present an overview of the pathfinder Palomar Gattini-IR project, including the ambitious goal of sub-pixel imaging and ramifications of this goal on the opto-mechanical design and data reduction software. Palomar Gattini-IR will pave the way for a dual hemisphere, infrared-optimized, ultra-wide field high cadence machine called Turbo Gattini-IR. To take advantage of the low sky background at 2.5 μm, two identical systems will be located at the polar sites of the South Pole, Antarctica and near Eureka on Ellesmere Island, Canada. Turbo Gattini-IR will survey 15,000 sq. degrees to a depth of 20AB, the same depth of the VISTA VHS survey, every 2 hours with a survey efficiency of 97%

Manipulating ultracold atoms with a reconfigurable nanomagnetic system of domain walls

The divide between the realms of atomic-scale quantum particles and lithographically-defined nanostructures is rapidly being bridged. Hybrid quantum systems comprising ultracold gas-phase atoms and substrate-bound devices already offer exciting prospects for quantum sensors, quantum information and quantum control. Ideally, such devices should be scalable, versatile and support quantum interactions with long coherence times. Fulfilling these criteria is extremely challenging as it demands a stable and tractable interface between two disparate regimes. Here we demonstrate an architecture for atomic control based on domain walls (DWs) in planar magnetic nanowires that provides a tunable atomic interaction, manifested experimentally as the reflection of ultracold atoms from a nanowire array. We exploit the magnetic reconfigurability of the nanowires to quickly and remotely tune the interaction with high reliability. This proof-of-principle study shows the practicability of more elaborate atom chips based on magnetic nanowires being used to perform atom optics on the nanometre scale.Comment: 4 pages, 4 figure

Ubiquitin Ligase RNF146 Regulates Tankyrase and Axin to Promote Wnt Signaling

Canonical Wnt signaling is controlled intracellularly by the level of β-catenin protein, which is dependent on Axin scaffolding of a complex that phosphorylates β-catenin to target it for ubiquitylation and proteasomal degradation. This function of Axin is counteracted through relocalization of Axin protein to the Wnt receptor complex to allow for ligand-activated Wnt signaling. AXIN1 and AXIN2 protein levels are regulated by tankyrase-mediated poly(ADP-ribosyl)ation (PARsylation), which destabilizes Axin and promotes signaling. Mechanistically, how tankyrase limits Axin protein accumulation, and how tankyrase levels and activity are regulated for this function, are currently under investigation. By RNAi screening, we identified the RNF146 RING-type ubiquitin E3 ligase as a positive regulator of Wnt signaling that operates with tankyrase to maintain low steady-state levels of Axin proteins. RNF146 also destabilizes tankyrases TNKS1 and TNKS2 proteins and, in a reciprocal relationship, tankyrase activity reduces RNF146 protein levels. We show that RNF146, tankyrase, and Axin form a protein complex, and that RNF146 mediates ubiquitylation of all three proteins to target them for proteasomal degradation. RNF146 is a cytoplasmic protein that also prevents tankyrase protein aggregation at a centrosomal location. Tankyrase auto-PARsylation and PARsylation of Axin is known to lead to proteasome-mediated degradation of these proteins, and we demonstrate that, through ubiquitylation, RNF146 mediates this process to regulate Wnt signaling

Caveolin-1 Plays a Crucial Role in Inhibiting Neuronal Differentiation of Neural Stem/Progenitor Cells via VEGF Signaling-Dependent Pathway

In the present study, we aim to elucidate the roles of caveolin-1(Cav-1), a 22 kDa protein in plasma membrane invaginations, in modulating neuronal differentiation of neural progenitor cells (NPCs). In the hippocampal dentate gyrus, we found that Cav-1 knockout mice revealed remarkably higher levels of vascular endothelial growth factor (VEGF) and the more abundant formation of newborn neurons than wild type mice. We then studied the potential mechanisms of Cav-1 in modulating VEGF signaling and neuronal differentiation in isolated cultured NPCs under normoxic and hypoxic conditions. Hypoxic embryonic rat NPCs were exposed to 1% O2 for 24 h and then switched to 21% O2 for 1, 3, 7 and 14 days whereas normoxic NPCs were continuously cultured with 21% O2. Compared with normoxic NPCs, hypoxic NPCs had down-regulated expression of Cav-1 and up-regulated VEGF expression and p44/42MAPK phosphorylation, and enhanced neuronal differentiation. We further studied the roles of Cav-1 in inhibiting neuronal differentiation by using Cav-1 scaffolding domain peptide and Cav-1-specific small interfering RNA. In both normoxic and hypoxic NPCs, Cav-1 peptide markedly down-regulated the expressions of VEGF and flk1, decreased the phosphorylations of p44/42MAPK, Akt and Stat3, and inhibited neuronal differentiation, whereas the knockdown of Cav-1 promoted the expression of VEGF, phosphorylations of p44/42MAPK, Akt and Stat3, and stimulated neuronal differentiation. Moreover, the enhanced phosphorylations of p44/42MAPK, Akt and Stat3, and neuronal differentiation were abolished by co-treatment of VEGF inhibitor V1. These results provide strong evidence to prove that Cav-1 can inhibit neuronal differentiation via down-regulations of VEGF, p44/42MAPK, Akt and Stat3 signaling pathways, and that VEGF signaling is a crucial target of Cav-1. The hypoxia-induced down-regulation of Cav-1 contributes to enhanced neuronal differentiation in NPCs

Streptococcus iniae M-Like Protein Contributes to Virulence in Fish and Is a Target for Live Attenuated Vaccine Development

Streptococcus iniae is a significant pathogen in finfish aquaculture, though knowledge of virulence determinants is lacking. Through pyrosequencing of the S. iniae genome we have identified two gene homologues to classical surface-anchored streptococcal virulence factors: M-like protein (simA) and C5a peptidase (scpI).S. iniae possesses a Mga-like locus containing simA and a divergently transcribed putative mga-like regulatory gene, mgx. In contrast to the Mga locus of group A Streptococcus (GAS, S. pyogenes), scpI is located distally in the chromosome. Comparative sequence analysis of the Mgx locus revealed only one significant variant, a strain with an insertion frameshift mutation in simA and a deletion mutation in a region downstream of mgx, generating an ORF which may encode a second putative mga-like gene, mgx2. Allelic exchange mutagenesis of simA and scpI was employed to investigate the potential role of these genes in S. iniae virulence. Our hybrid striped bass (HSB) and zebrafish models of infection revealed that M-like protein contributes significantly to S. iniae pathogenesis whereas C5a peptidase-like protein does not. Further, in vitro cell-based analyses indicate that SiMA, like other M family proteins, contributes to cellular adherence and invasion and provides resistance to phagocytic killing. Attenuation in our virulence models was also observed in the S. iniae isolate possessing a natural simA mutation. Vaccination of HSB with the Delta simA mutant provided 100% protection against subsequent challenge with a lethal dose of wild-type (WT) S. iniae after 1,400 degree days, and shows promise as a target for live attenuated vaccine development.Analysis of M-like protein and C5a peptidase through allelic replacement revealed that M-like protein plays a significant role in S. iniae virulence, and the Mga-like locus, which may regulate expression of this gene, has an unusual arrangement. The M-like protein mutant created in this research holds promise as live-attenuated vaccine