3 research outputs found
Multicultural Education: More than the Acknowledgement of Black History Month
I remember clearly as a child, in the early nineteen sixties, the only African American mentioned in my school was Crispus Attucks. For a long time I thought there was only one black man that fought in the entire revolutionary war. From the big old musty textbooks we were given to study it was obvious very few black people had accomplished anything worth writing about. I hated history. I thought its only concern were old dead white men. Understandably, I did not do well in history. Since 2005, enrollment in public schools has increased, but “Black males continue spiraling down the achievement ladder” (Whiting 2006). All children need to see themselves in the context of what they are learning (Stiler & Allen, 2006). They need to know that many of the things that affect current day thinking and living have emerged from an amalgamation of peoples. It is important for the students’ self-esteem and often can mean the difference between ritual compliance and full engagement (Baker, 2005)
The Effect of Sample Handling on Cross Sectional HIV Incidence Testing Results
To determine if mishandling prior to testing would make a sample from a chronically infected subject appear recently infected when tested by cross-sectional HIV incidence assays.Serum samples from 31 subjects with chronic HIV infection were tested. Samples were subjected to different handling conditions, including incubation at 4 °C, 25 °C and 37 °C, for 1, 3, 7 or 15 days prior to testing. Samples were also subjected to 1,3, 7 and 15 freeze-thaw cycles prior to testing. Samples were tested using the BED capture enzyme immuno assay (BED-CEIA), Vironostika-less sensitive (V-LS), and an avidity assay using the Genetic Systems HIV-1/HIV-2 plus O EIA (avidity assay).Compared to the sample that was not subjected to any mishandling conditions, for the BED-CEIA, V-LS and avidity assay, there was no significant change in test results for samples incubated at 4 °C or 25 °C prior to testing. No impact on test results occurred after 15 freeze-thaw cycles. A decrease in assay results was observed when samples were held for 3 days or longer at 37 °C prior to testing.Samples can be subjected up to 15 freeze-thaw cycles without affecting the results the BED-CEIA, Vironostika-LS, or avidity assays. Storing samples at 4 °C or 25 °C for up to fifteen days prior to testing had no impact on test results. However, storing samples at 37°C for three or more days did affect results obtained with these assays