Vacuum-UV negative photoion spectroscopy of CH4

Using synchrotron radiation in the range 12-35 eV, negative ions are detected by mass spectrometry following vacuum-UV photoexcitation of methane. Ion yields for H$^-$, CH$^-$ and CH$_2^-$ are recorded, the spectra of CH$^-$ and CH$_2^-$ for the first time. All ions display a linear dependence of signal with pressure, showing that they arise from unimolecular ion-pair dissociation. Cross sections for ion-pair formation are put onto an absolute scale by calibrating the signal strengths with those of F$^-$ from SF$_6$ and CF$_4$. Following normalisation to total vacuum-UV absorption cross sections, quantum yields for anion production are reported. There is a major discrepancy in the H$^-$ cross section with an earlier measurement, which remains unresolved. The anions arise from both direct and indirect ion-pair mechanisms. For a generic polyatomic molecule AB, the former is defined as AB $\rightarrow$ A$^-$ + B$^+$ (+ neutrals), the latter as the predissociative crossing of an initially-excited Rydberg state of AB by an ion-pair state. In a separate experiment, the threshold photoelectron spectrum of the second valence band of CH$_4$, ionisation to CH$_4^+$ A $^2$A$_1$ at 22.4 eV, is recorded with an instrumental resolution of 0.004 eV; many of the Rydberg states observed in indirect ion-pair formation converge to this state. The widths of the peaks are lifetime limited, increasing with increasing $v$ in the $v_1$ (a$_1$) vibrational ladder. They are the first direct measurement of an upper value to the dissociation rate of these levels into fragment ions

An X-Ray + Radio Search for Massive Black Holes in Blue Compact Dwarf Galaxies

Nearby blue compact dwarf (BCD) galaxies are arguably our best local analogues of galaxies in the earlier Universe that may host relics of black hole (BH) seeds. Here we present high-resolution Chandra X-ray Observatory and Karl G. Jansky Very Large Array (VLA) observations of five nearby BCDs with stellar masses of less than the Small Magellanic Cloud ($M_\star \sim 10^{7} - 10^{8.4}$ $M_\odot$). We search for signatures of accreting massive BHs at X-ray and radio wavelengths, which are more sensitive to lower BH accretion rates than optical searches. We detect a total of 10 hard X-ray sources and 10 compact radio sources at luminosities consistent with star-formation-related emission. We find one case of a spatially-coincident X-ray and radio source within the astrometric uncertainties. If the X-ray and radio emission are indeed coming from the same source, the origin of the radiation is plausibly from an active massive BH with log $(M_{\rm BH}/M_{\odot}) \sim 4.8 \pm 1.1$. However, given that the X-ray and radio emission are also coincident with a young star cluster complex, we consider the combination of an X-ray binary and a supernova remnant (or \HII\ region) a viable alternative explanation. Overall, we do not find compelling evidence for active massive BHs in our target BCDs, which on average have stellar masses more than an order of magnitude lower than previous samples of dwarf galaxies found to host massive BHs. Our results suggest that moderately accreting massive BHs in BCDs are not so common as to permit unambiguous detection in a small sample.Comment: 13 pages, 4 figures, accepted for publication in Ap

Stimulation of MAP kinase by v-raf transformation of fibroblasts fails to induce hyperphosphorylation of transfected tau

AbstractA proportion of the microtubule-associated protein, tau, is in an elevated state of phosphorylation in foetal and adult brain whereas all of the tau in paired helical filaments, which are characteristic of Alzheimer's disease is hyperphosphorylated; it is important therefore to elucidate the mechanisms that regulate tau phosphorylation. Here we describe results that show that although MAP kinase can hyperphosphorylate tau in vitro, activation of MAP kinase in transformed fibroblasts does not result in hyperphosphorylation of transfected tau, whereas glycogen synthase kinase-3β (GSK-3β) when co-transfected with tau does result in tau hyperphosphorylation. The findings imply that GSK-3β may be a stronger candidate than MAP kinase for inducing tau hyperphosphorylation in vivo