290 research outputs found

    Is banking a declining industry? A historical perspective

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    Banks and banking ; Bank assets ; Financial institutions

    Partnering in Great Plains Regional GAP

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    Partnering within the Great Plains states has been very important to the success of the Great Plains Regional GAP project. Over $3.5 million have been received in monetary and in-kind support from partners for the direct funding of basic layers of gap analysis as well as spin-offs of the GAP projects important to partners in the Great Plains (Table 1). Successes in basic gap analysis efforts would not have been possible without the direct and financial support of our many partners in the Great Plains. In addition to the financial aspects of partnering, contributions of partners have demonstrated their interest in our work and the value of creating high-quality, state-of-the-art products. Our partnering support, both in direct and in-kind financial aspects, also has stimulated several spin-off projects within the basic GAP effort, thereby facilitating future partnering with various agencies and organizations

    The human macrophage mannose receptor directs Mycobacterium tuberculosis lipoarabinomannan-mediated phagosome biogenesis

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    Mycobacterium tuberculosis (M.tb) survives in macrophages in part by limiting phagosome–lysosome (P-L) fusion. M.tb mannose-capped lipoarabinomannan (ManLAM) blocks phagosome maturation. The pattern recognition mannose receptor (MR) binds to the ManLAM mannose caps and mediates phagocytosis of bacilli by human macrophages. Using quantitative electron and confocal microscopy, we report that engagement of the MR by ManLAM during the phagocytic process is a key step in limiting P-L fusion. P-L fusion of ManLAM microspheres was significantly reduced in human macrophages and an MR-expressing cell line but not in monocytes that lack the receptor. Moreover, reversal of P-L fusion inhibition occurred with MR blockade. Inhibition of P-L fusion did not occur with entry via Fcγ receptors or dendritic cell–specific intracellular adhesion molecule 3 grabbing nonintegrin, or with phosphatidylinositol-capped lipoarabinomannan. The ManLAM mannose cap structures were necessary in limiting P-L fusion, and the intact molecule was required to maintain this phenotype. Finally, MR blockade during phagocytosis of virulent M.tb led to a reversal of P-L fusion inhibition in human macrophages (84.0 ± 5.1% vs. 38.6 ± 0.6%). Thus, engagement of the MR by ManLAM during the phagocytic process directs M.tb to its initial phagosomal niche, thereby enhancing survival in human macrophages
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