High prevalence of human papillomavirus (HPV) in oral mucosal lesions of patients at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Northeastern Brazil

The role of human papillomavirus (HPV) in oral carcinogenesis is still controversial as detection rates of the virus in oral cavity reported in the literature varies greatly. Objective The aim of this study was to evaluate the frequency of HPV infection and its genotypes in patients with oral lesions at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Brazil. Material and Methods We conducted a molecular study with 21 patients (15 females) aged from two to 83 years with clinically detectable oral lesions. Samples were collected through exfoliation of lesions and HPV-DNA was identified using MY09/11 and GP5+/6+ primers. Genotyping was performed by multiplex PCR. Results Benign, premalignant and malignant lesions were diagnosed by histopathology. HPV was detected in 17 samples. Of these, HPV-6 was detected in 10 samples, HPV-18 in four and HPV-16 in one sample. When samples were categorized by lesion types, HPV was detected in two papilloma cases (2/3), five carcinomas (5/6), one hyperplasia (1/1) and nine dysplasia cases (9/11). Conclusion Unlike other studies in the literature, we reported high occurrence of HPV in oral lesions. Further studies are required to enhance the comprehension of natural history of oral lesions

Perfil de citocinas no soro e na secreção cervical de mulheres com lesão intraepitelial de baixo grau, lesão intraepitelial de alto grau e carcinoma epidermóide invasor

A infecção por Papilomavírus Humano (HPV) oncogênico é o fator de risco mais importante para desenvolvimento de lesões intraepiteliais bem como para o carcinoma epidermóide invasor. A resposta imune tem um papel importante na história natural da infecção pelo HPV na cérvice uterina estando envolvida na persistência viral e na progressão das lesões. As citocinas são moléculas reguladoras da transcrição do HPV, sendo que o padrão Th1 contribui para o desenvolvimento da imunidade celular contra a neoplasia e infecção pelo HPV, e está relacionado com clearance dessa infecção. O padrão Th2 está associado com a persistência da infecção viral e com a progressão das lesões. Avaliar a concentração de IL-2, IL-4, IL-6, IL-10, IL-12, IFN-g e TNF-a na secreção cervical e no soro de mulheres portadoras de lesão intraepitelial de baixo grau (LIEBG), lesão intraepitelial de alto grau (LIEAG) e carcinoma epidermóide invasor (CE). Foram incluídas no estudo 109 mulheres com diagnóstico histopatológico de LIEBG (n=16), LIEAG (n=40), CE (n=13) e 40 mulheres com suspeita de doença HPV induzida, mas sem alterações histopatológicas na biópsia do colo do útero (grupo controle), atendidas no Ambulatório de Colposcopia da Faculdade de Medicina de Botucatu (UNESP) e no Ambulatório de Ginecologia Preventiva do Hospital Amaral Carvalho, Jaú, SP. Durante o exame especular foi coletada a secreção cervical com cytobrush para determinação do perfil de citocinas. O sangue periférico das mulheres incluídas no estudo foi colhido por punção venosa, em volume de 10mL e os padrões de citocinas no soro e na secreção cervical foram avaliados por ensaio imunoenzimático (ELISA). A pesquisa de HPV nas biópsias foi realizada empregando-se a técnica da reação em cadeia da polimerase (PCR) utilizando os primers específicos (MY9/11 e GP5+/GP6+) e a genotipagem foi realizada...Infection with oncogenic types of human papillomavirus (HPV) is the most important factor for development of intraepithelial lesions and invasive squamous cell carcinoma. The immune response has an important role in the natural history of HPV infection in uterine cervix is involved in viral persistence and progression of lesions. Cytokines are molecules that regulate the transcription of HPV; the Th1 cytokine profile contributes to the development of cellular immunity against HPV infection and neoplasia, and is related to clearance of infection. The Th2 cytokine profile is associated with persistence of viral infection and the progression of lesions. Objectve: To evaluate the concentration of IL-2, IL-4, IL-6, IL- 10, IL-12, IFN-g and TNF-a in cervical secretion and serum of women with low-grade squamous intraepithelial lesions (LSIL), high-grade intraepithelial lesion (HSIL) and invasive squamous cell carcinoma (EC). The study included 109 women with histological diagnosis of LSIL (n = 16), HSIL (n = 40), EC (n = 13) and 40 women with suspected of HPVinduced disease, but no pathological changes on cervix biopsy (control group) attending in the Colposcopy Clinic of the Botucatu Medical School (UNESP) and in the Preventive Gynecology, Hospital Amaral Carvalho, Jaú, SP. During speculum examination cervical secretion was collected with cytobrush to determining the profile of cytokines. The peripheral blood of women enrolled in the study was collected by venipuncture in 10 mL volume and patterns of cytokines in serum and cervical secretion were assessed by enzyme immunoassay (ELISA). The search for HPV in the fragments of the biopsies were performed using the technique of polymerase chain reaction (PCR) using specific primers (MY9/11 and GP5+/GP6+) and genotyping was performed using the technique of PCR primers specific and confirmed by the analysis of restriction fragment polymorphism... (Complete abstract click electronic access below)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Análise de carga viral e estado físico do Papilomavírus Humano (HPV) e perfil de citocinas em mulheres com lesão intraepitelial cervical e carconoma cervical invasor

Introduction: Infection with high-risk genotypes of the human papillomavirus (HPV) is associated with the development of preinvasive cervical lesions and invasive cervical carcinoma. The viral load and physical state of HPV genotypes are considered important biomarkers for lesion progression. The immune response has an important role in the natural history of HPV infection in the uterine cervix, as, for example, the viral persistence and the development of cervical cancer. Cytokines are the main mediators of this immune response and they regulate the HPV transcription depending on the response pattern to which they are related. The Th1 pattern contributes to the development of cellular immunity against HPV infection and its clearance. On the other hand, Th2 pattern is associated with the persistence of viral infection and the progression of cervical lesions. Most of these infections probably involves a balance between Th1 and Th2 immune response profiles.Objective: To evaluate the concentration of IL-1 β, IL-4, IL-6, IL-10,TNF-and INF-in secretionand serum and also to assess the immunostaining intensity of these cytokines in cervical biopsies.Moreover, the present study aimed to determine the viral load and physical state of HPV16 in women with low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL), invasive cervical carcinoma (ICC) and also in the control group.Patients and Methods: This study included 109 women with histopathologic diagnosis of LSIL (n=16), HSIL (n=40), ICC (n=13) and 40 women with no pathological changes on cervix biopsy (control group) attended in Preventive Gynaecological Ambulatory at the Amaral Carvalho Hospital, Jaú, São Paulo, Brazil. Cytokine concentrationwas determined in serum and cervical secretion by enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry was used to evaluate the immunostaining intensity of cytokines in biopsy ...Introdução: A infecção por genótipos de alto risco doPapilomavírus Humano (HPV) está associada ao desenvolvimento das lesões cervicais pré-invasivas e do carcinoma cervical invasor. A carga viral e o estado físico desses genótipos de HPV são considerados importantes biomarcadores para a progressão dessas lesões. A resposta imune do hospedeiro tem um papel importante na história natural da infecção pelo HPV na cérvice uterina como, por exemplo, a persistência viral e o desenvolvimento do câncer cervical. As citocinas são os principais mediadores desta resposta imune e são capazes de regular a transcrição do HPV dependendo do padrão de resposta ao qual estão associadas. O padrão Th1 contribui para o desenvolvimento da imunidade celular contra a infecção pelo HPV e está relacionado com clearance dessa infecção.Por outro lado, o padrão Th2 está associado com a persistência viral da infecção e com a progressão das lesões cervicais. A maioria dessas infecções provavelmente envolve um balanço entre osperfis Th1 e Th2 da resposta imune. Objetivo: Avaliar a concentração de IL-1β, IL-4, IL-6, IL-10, TNF-e INF- na secreção e no soro e a intensidade da imunomarcação destas citocinas nas biópsias cervicais. Além disso, determinar a carga viral e o estado físico do HPV16 em mulheres portadoras de lesão intraepitelial escamosa de baixo grau (LIEBG), lesão intraepitelial escamosa de alto grau (LIEAG), carcinoma cervical invasor (CCI) e no grupo controle.Pacientes e Métodos: Foram incluídas no estudo 109 mulheres com diagnóstico histopatológico de LIEBG (n=16), LIEAG (n=40), CCI (n=13) e 40 mulheres sem alterações histopatológicas na biópsia do colo do útero (grupo controle), atendidas no Ambulatório de Ginecologia Preventiva do Hospital Amaral Carvalho, Jaú, SP. As concentrações das diferentes citocinasno soro e na secreção cervical foram determinadas por ensaio imunoenzimático...Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis

This study was undertaken to evaluate the prophylactic potential of proteoglycan (PG) administration in experimental arthritis. Female BALB/c retired breeder mice received two (2xPG50 and 2xPG100 groups) or three (3xPG50 group) intraperitoneal doses of bovine PG (50 μg or 100 μg) every three days. A week later the animals were submitted to arthritis induction by immunization with three i.p. doses of bovine PG associated with dimethyldioctadecylammonium bromide adjuvant at intervals of 21 days. Disease severity was daily assessed after the third dose by score evaluation. The 3xPG50 group showed significant reduction in prevalence and clinical scores. This protective effect was associated with lower production of IFN-γ and IL-17 and increased production of IL-5 and IL-10 by spleen cells restimulated in vitro with PG. Even though previous PG administration restrained dendritic cells maturation this procedure did not alter the frequency of regulatory Foxp3+ T cells. Lower TNF-α and IL-6 levels and higher expression of ROR-γ and GATA-3 were detected in the paws of protected animals. A delayed-type hypersensitivity reaction confirmed specific tolerance induction. Taken together, these results indicate that previous PG inoculation determines a specific tolerogenic effect that is able to decrease severity of subsequently induced arthritis

High prevalence of human papillomavirus (HPV) in oral mucosal lesions of patients at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Northeastern Brazil

Abstract The role of human papillomavirus (HPV) in oral carcinogenesis is still controversial as detection rates of the virus in oral cavity reported in the literature varies greatly. Objective The aim of this study was to evaluate the frequency of HPV infection and its genotypes in patients with oral lesions at the Ambulatory of Oral Diagnosis of the Federal University of Sergipe, Brazil. Material and Methods We conducted a molecular study with 21 patients (15 females) aged from two to 83 years with clinically detectable oral lesions. Samples were collected through exfoliation of lesions and HPV-DNA was identified using MY09/11 and GP5+/6+ primers. Genotyping was performed by multiplex PCR. Results Benign, premalignant and malignant lesions were diagnosed by histopathology. HPV was detected in 17 samples. Of these, HPV-6 was detected in 10 samples, HPV-18 in four and HPV-16 in one sample. When samples were categorized by lesion types, HPV was detected in two papilloma cases (2/3), five carcinomas (5/6), one hyperplasia (1/1) and nine dysplasia cases (9/11). Conclusion Unlike other studies in the literature, we reported high occurrence of HPV in oral lesions. Further studies are required to enhance the comprehension of natural history of oral lesions

Elevated prevalence of high-risk HPV in healthy oral mucosa of users and nonusers of drugs in Northeastern Brazil

Abstract: Variable rates of HPV infection have been reported in healthy oral mucosa worldwide. The main objective of this study was to detect and genotype HPV infection in users and nonusers of drugs with clinically healthy mucosa from the Northeast Brazil. Samples from 105 patients were amplified using the primers MY09/MY11 and GP5+/GP6+, and genotyping was performed by multiplex-PCR for HPV-6/11, 16 and 18. A total of 81.9% samples were positive. Among drug users, 84.5% presented the virus and 20.4% showed multiple infections. Among non-drug users, 78.7% were positive and 13.5% had multiple infections. Limited information is available on oral HPV in Brazilian population, especially for drug users, and our results showed higher HPV infection rates in both users and nonusers of drugs. More studies and researches focused on drug users including factors like sexual behavior, nutrition and cultural habits are necessary to enhance the comprehension of this relationship, and develop preventive strategies