A Multifunctional Bread Rich in Beta Glucans and Low in Starch Improves Metabolic Control in Type 2 Diabetes: A Controlled Trial

Abstract: Design: Functional foods may be useful for people with diabetes. The soluble fibers beta glucans can modify starch digestion and improve postprandial glucose response. We analyzed the metabolic effects of a specifically designed \u2018functional\u2019 bread, low in starch, rich in fibers (7 g/100 g), with a beta glucan/starch ratio of (7.6:100, g/g), in people with type 2 diabetes mellitus. Methods: Clinical and metabolic data from two groups of age-, sex- and glycated hemoglobin-matched diabetic subjects, taking either the functional bread or regular white bread, over a roughly six-month observation period, were retrieved. Results: Bread intake did not change during the trial. The functional bread reduced glycated hemoglobin by ~0.5% (absolute units) vs. pre-treatment values (p = 0.028), and by ~0.6% vs. the control group (p = 0.027). Post-prandial and mean plasma glucose was decreased in the treatment group too. Body weight, blood pressure and plasma lipids did not change. The acceptance of the functional bread was good in the majority of subjects, except for taste. Conclusions: A starch-restricted, fiber-rich functional bread, with an increased beta glucan/starch ratio, improved long term metabolic control, and may be indicated in the dietary treatment of type 2 diabetes

Immobilization of Bacteriocins from Lactic Acid Bacteria and Possibilities for Application in Food Biopreservation

Bacteriocins are biologically active compounds produced by a large number of bacteria, including lactic acid bacteria (LAB), which exhibit antimicrobial activity against various saprophytic and pathogenic microorganisms. In recent decades, bacteriocins are increasingly becoming more important in different branches of the industry due to their broad antibacterial and antifungal spectrum - in the food industry for natural food preservation and expiry date extension; in the health sector for preparation of probiotic foods and beverages; in the clinical practice as alternatives of conventional antibiotics; in the agriculture as biocontrol agents of plant pathogens and alternatives of chemical pesticides for plant protection. The broad antimicrobial spectrum of bacteriocins has stimulated the research attention on their application mainly in the food industry as natural preservatives. Most scientific achievements concerning the application food biopreservation are related to bacteriocins produced by LAB. The lactic acid bacteria bacteriocins can be produced in the food substrate during its natural fermentation or can be added in the food products after obtaining by in vitro fermentations under optimal physical and chemical conditions. Moreover, the immobilization of LAB bacteriocins on different matrices of organic and inorganic origin has been proposed as an advanced approach in the natural food preservation for their specific antimicrobial activity, anti-biofilm properties and potential use as tools for pathogen detection

UV-A light treatment for controlling enzymatic browning of fresh-cut fruits

Enzymatic browning is critical in the preservation of the quality and the shelf-life of fresh-cut fruits. Non-thermal technologies such as UV radiation are emerging for controlling polyphenol oxidase (PPO) activity, the main agent responsible for browning. After determining the best operational conditions of a UV LED illuminator (2.43\ub710-3 Wm-2 irradiance), the anti-browning effect of UV-A light (390 nm) treatment at 25 \ub0C over increasing time periods up to 60 min was assessed on fresh-cut apples (Golden Delicious, Granny Smith, Fuji) and pears (Abate F\ue9tel, Decana). Colour variation (\u394E) and its percent reduction (%R\u394E) were measured using a colorimeter and the greatest effect was observed in apples which showed higher %R\u394E values than pears (58% vs 25% after 60 min exposure, respectively). Moreover electrophoretic and zymographic techniques demonstrated the inhibitory effect of UV-A irradiation on PPO activity. Anti-browning was found to be related to irradiance, exposure time and the fruit cultivar. Overall this study confirmed UV-A LED technology as an eco-friendly alternative to traditional approaches for reducing browning of minimally processed products. Industrial relevance: Treatment of fresh-cut products with UV-A LED is not only easy and inexpensive to produce but also presents few hazards for humans. Moreover, the use of LED light sources brings many advantages such as energy savings, device durability, low environmental impact, high luminous efficiency and little thermal effect. UV-A LED technology has great potential to meet the demands of the food industry in the processing of fresh-cut fruits and vegetables. In addition it could also be considered a pre-treatment of fruits and vegetables being processed for the production of snacks

Essential amino acids: master regulators of nutrition and environmental footprint?

The environmental footprint of animal food production is considered several-fold greater than that of crops cultivation. Therefore, the choice between animal and vegetarian diets may have a relevant environmental impact. In such comparisons however, an often neglected issue is the nutritional value of foods. Previous estimates of nutrients\u2019 environmental footprint had predominantly been based on either food raw weight or caloric content, not in respect to human requirements. Essential amino acids (EAAs) are key parameters in food quality assessment. We re-evaluated here the environmental footprint (expressed both as land use for production and as Green House Gas Emission (GHGE), of some animal and vegetal foods, titrated to provide EAAs amounts in respect to human requirements. Production of high-quality animal proteins, in amounts sufficient to match the Recommended Daily Allowances of all the EAAs, would require a land use and a GHGE approximately equal, greater o smaller (by only \ub11-fold), than that necessary to produce vegetal proteins, except for soybeans, that exhibited the smallest footprint. This new analysis downsizes the common concept of a large advantage, in respect to environmental footprint, of crops vs. animal foods production, when human requirements of EAAs are used for reference

Water an Eco-Friendly Crossroad in Green Extraction: An Overview

In this overview, the function of water and the increasing role of water as a green solvent and co-extractant based on its application in ancient times and the need of environmental thinking have been discussed. A brief summary of various extraction methods for natural products, the application of conventional and innovative processes, based on water and future insights and perspectives considering water as an eco-friendly crossroad in green extraction have been reported. Taking into account also the issue of wastewater, this paper calls for more effective use of water as a finite resource

Biopreservation of Fresh Strawberries by Carboxymethyl Cellulose Edible Coatings Enriched with a Bacteriocin of Bacillus methylotrophicus BM47

Bacteriocins are a large group of antimicrobial compoundsthat aresynthesized byrepresentatives ofthegenus Bacillusand lactic acid bacteria (LAB). Bacteriocinsare used extensively in thefood industry as biopreservatives. Incorporatedin the composition of edible coatings, bacteriocins canreduce microbial growthand decay incidencein perishable fruits, thusimproving productshelf-life and commercial appearance. The present study aims to investigatethe effect of edible coatings of0.5% carboxymethyl cellulose (CMC) enriched with a purified bacteriocin fromBacillus methylotrophicusBM47 on the shelf-life extension of fresh strawberries. During storage at 4\ub0Cand 75 % relative humidity(RH) for 16 days, measurements of massloss, decay percentage, total soluble solids(TSS), titratable acidity(TA),pH, organic acids, total phenolic and anthocyaninscontents and antioxidant activity were taken.The results demonstrated that the application of 0.5% CMC and 0.5% CMC+bacteriocin (CMC+B) edible coatings led to a significant decrease of massloss in treated strawberriescompared to the uncoated fruit. After the 8-th day of storage, significant reductions in decay percentage along with the absence of fungal growth in CMC+B-coated fruit wereobserved in comparison to the CMC-coated and control strawberries. During the second half of the storage period, CMC and CMC+B treatmentsreduced TSS levels inthe coated fruitcompared to the control, but did not affectincreasedTA and loweredpH valuesthat arenormally associated with post-harvest changes. The CMC and CMC+B coatings wereineffectiveagainst thedecrease inascorbic acid, total phenolics and anthocyanins contentduringcold storage. The application of CMC and CMC+B coatingsexhibited a significant inhibitory effect on decreasing antioxidant activity throughout the storage period and maintained the antioxidant levels inboth treatmentsclose to the initial value of 76.8 mmol TE/100 g of fm

Polyphenols as Suitable control for Obesity and Diabetes

Modern life is characterized by physical inactivity and poor food choices, which is often a prerequisite for unhealthy weight gain and overweight/obesity. These factors unlock the emergence of a number of diseases including diabetes, cardiovascular problems, different types of cancer, etc. The pursuit of scientists to seek strategies to prevent, relieve and cure the patient leads to the usage of natural compounds of potential beneficial effect. Polyphenols are a large group of naturally occurring secondary metabolites mainly found in plants and beverages. The presence of these secondary metabolites seems to decrease the manifestation of miscellaneous disease-causing symptoms. The purpose of this review is to synthesize information about polyphenols and their potential in controlling obesity and diabetes. Polyphenols are considered as health-beneficial sources and thus could be involved in novel strategies for preventing diabetes and obesity complications

Insights and Perspectives on Plant-Based Beverages

The emerging demand for everyday food substitutes is increasing daily. More and more individuals struggle with allergies and intolerances, making providing alternatives for simple products like dairy milk mandatory. Plant-based beverages (PBBs) are currently trending due to the multiple diets that promote their consumption with or without a justification. PBBs can derive from various types of plants, not exclusively nuts. Some of the most well-known sources are almonds, soy, rice, and hazelnuts. Given the need for sustainable approaches to resource utilization and food production, novel sources for PBBs are being sought, including fruit kernels. The plant kingdom offers a palette of resources with proven bioactivity, i.e., containing flavonoids, phenolic acids, vitamins, carotenoids, and phenolics. Many of these beneficial substances are water-soluble, which could be transferred to the plant beverage compositions. The current review compares the vast number of potential formulations based on their specific nutritional profiles and possible deficiencies, as well as their expected health-promoting properties based on the raw material(s) used for production. Special attention will be given to the antinutrients, usually abundant in plant-based sources

Valorization of onion extracts as anti-browning agents

The enzymatic browning, whose main responsible is polyphenol oxidase (PPO, EC 1.14.18.1), is involved in the phenolic oxidation and colour alteration of minimally-processed fruits and vegetables. Currently, the research of new strategies to inactivate PPO is moving towards replacing synthetic additives such as organic acids and sulphites with natural inhibitors. The present study is focused on investigating the anti-browning performance of juices and distillates obtained from three onion varieties (white, yellow, and red) and Borettana onion wastes (inner layers). Their inhibitory activity on a commercial mushroom tyrosinase and some plant PPOs has been evaluated by spectrophotometric and electrophoretic analysis. The in vivo trials has been also carried out by monitoring over time at room temperature the colour change on potato slices under accelerated browning conditions. The effectiveness of onion samples in limiting enzymatic browning was affected by not only the enzyme source but also inhibitor type. Although distillates had higher anti-PPO capacity as confirmed by in vitro assays, juices showed better in vivo effectiveness. Hence, onions and their wastes can be valorised as a natural source of anti-browning agents to control PPO activity thus preserving sensory, antioxidant and nutritional properties of agro-food products

Antioxidant Properties of Soybean Oil Supplemented with Ginger and Turmeric Powders

Soybean oil has been supplemented with 10% (w/w) of ginger and turmeric powders derived from commercial products (GC—commercial ginger and TC—commercial turmeric), freeze-dried rhizomes (freeze-dried ginger (GR) and freeze-dried turmeric rhizome—TR) and peels (freeze-dried ginger peel (GP) and freeze-dried turmeric peel—TP) for developing a functional seasoning with great lipid stability for human consumption. The exhausted ginger and turmeric powders were also recovered and recycled two times to promote a more sustainable process. The antioxidant activity and oxidative stability of oil samples were evaluated respectively by spectrophotometric and Rancimat methods. Folin–Ciocalteu assay and HPLC analysis were also performed to quantify total polyphenols, ginger-derived 6-gingerol and 6-shogaol, and turmeric-derived curcumin. Their antioxidant activity as well as oxidative stability, which non-linearly decreased over cycles because of a strongly reduced phenolic extractability, linearly increased with increasing phenolic yields. Hence, ginger and turmeric can be proposed as healthy spices containing bioactive compounds to control lipid oxidation and improve oil stability. Moreover, the valorization of peels as eco-friendly source of natural antioxidants is a valid strategy for providing added-value to these agro-food wastes