Terahertz imaging with sub-wavelength resolution by femtosecond laser filament in air

Terahertz (THz) imaging provides cutting edge technique in biology, medical sciences and non-destructive evaluation. However, due to the long wavelength of the THz wave, the obtained resolution of THz imaging is normally a few hundred microns and is much lower than that of the traditional optical imaging. We introduce a sub-wavelength resolution THz imaging technique which uses the THz radiation generated by a femtosecond laser filament in air as the probe. This method is based on the fact that the femtosecond laser filament forms a waveguide for the THz wave in air. The diameter of the THz beam, which propagates inside the filament, varies from 20 {\mu}m to 50 {\mu}m, which is significantly smaller than the wavelength of the THz wave. Using this highly spatially confined THz beam as the probe, THz imaging with resolution as high as 20 {\mu}m (~{\lambda}/38) can be realized.Comment: 10 pages, 7 figure

An n-of-1 Trial Service in Clinical Practice: Testing the Effectiveness of Liuwei Dihuang Decoction for Kidney-Yin Deficiency Syndrome

Objective. To describe the clinical use of n-of-1 RCTs for kidney-Yin deficiency syndrome that is a traditional Chinese medicine syndrome in publicly clinical practice in China. Methods. Our study included patients with kidney-Yin deficiency syndrome, using a within-patient, randomized, double-blind, crossover comparison of Liuwei Dihuang decoction versus placebo. Outcome Measures. Primary outcome measures included number of individual completion rates, response rate, and post-n-of-1 RCTs decisions. Secondary measures were the whole group score of individual Likert scale, SF-36 questionnaire. Results. Fifty patients were recruited and 3 were not completed. Forty-seven patients completed 3 pairs of periods, 3 (6.38%) were responders, 28 (59.57%) were nonresponders, and 16 (34.05%) were possible responders. Doctors and patients used the trial results to making decision. Three responders stayed on the medication management, 28 nonresponders ceased the LDD, 7 patients of the 16 possible responders could not give clear decision, and the others kept the same medication station. Among the whole group, neither the individual Likert score nor the SF-36 showed any statistical differences between LDD and placebo. Discussion. More attention should be paid to choose experienced TCM doctor as investigator and keep the simulant same with test medication in n-of-1 RCTs of TCM and sufficiently biological half-life period of Chinese medicine compound

Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53

Pu-erh tea is a kind of fermented tea with the incorporation of microorganisms’ metabolites. Unlike green tea, the chemical characteristics and bioactivities of Pu-erh tea are still not well understood. Using water extracts of Pu-erh tea, we analyzed the tumor cell growth inhibition activities on several genetically engineered mouse tumor cell lines. We found that at the concentration that did not affect wild type mouse embryo fibroblasts (MEFs) growth, Pu-erh tea extracts could inhibit tumor cell growth by down-regulated S phase and cause G1 or G2 arrest. Further study showed that Pu-erh tea extracts down-regulated the expression of mutant p53 in tumor cells at the protein level as well as mRNA level. The same concentration of Pu-erh tea solution did not cause p53 stabilization or activation of its downstream pathways in wild type cells. We also found that Pu-erh tea treatment could slightly down-regulate both HSP70 and HSP90 protein levels in tumor cells. These data revealed the action of Pu-erh tea on tumor cells and provided the possible mechanism for Pu-erh tea action, which explained its selectivity in inhibiting tumor cells without affecting wild type cells. Our data sheds light on the application of Pu-erh tea as an anti-tumor agent with low side effects

The Gain of Function of p53 Mutant p53S in Promoting Tumorigenesis by Cross-talking with H-RasV12

The loss of wild type p53 tumor suppressive function and oncogenic gain-of-function of p53 mutants have been showing important implications in tumorigenesis. The p53N236S (p53N239S in human, p53S) mutation has been shown to lose wild type p53 function by yeast assay. However, its gain of function is still not clear. By gel shift assay, we showed that mutant p53S had lost its DNA binding ability to its target promoters. Further real-time PCR data confirmed that p53S had lost the function of regulating the transcription of p21 Cip1/Waf1, cyclin G, PUMA, and Bax in response to 10Gy irradiation. These data confirmed the loss of function of p53S in mammalian cells. By xenograft assay, we showed that the p53S per se was not oncogenic enough to form tumor, however, cooperating with H-RasV12, p53S could dramatically promote tumorigenesis in p53 null MEFs. Further study showed that co-expression of p53S and H-RasV12 could increase the expression level of H-RasV12 and partially eliminate the elevation of stress response proteins such as Chk2, &#947;-H2AX, Hsp70, Rb, p16Ink4a caused by either p53S or H-RasV12. These data suggested that p53S cross-talked with H-RasV12 and reduced the cellular stress response to oncogenic signals, which facilitated the cell growth and tumorigenesis. Together these data provided the molecular basis for the cooperation of p53S and H-RasV12 and revealed the gain of function of p53S in cross-talking with H-RasV12. This study revealed an important aspect of gain of function for p53 mutant, therefore might shed light on the clinical strategy in targeting p53 mutant.</p

Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53

Abstract: Pu-erh tea is a kind of fermented tea with the incorporation of microorganisms’ metabolites. Unlike green tea, the chemical characteristics and bioactivities of Pu-erh tea are still not well understood. Using water extracts of Pu-erh tea, we analyzed the tumor cell growth inhibition activities on several genetically engineered mouse tumor cell lines. We found that at the concentration that did not affect wild type mouse embryo fibroblasts (MEFs) growth, Pu-erh tea extracts could inhibit tumor cell growth by down-regulated S phase and cause G1 or G2 arrest. Further study showed that Pu-erh tea extracts down-regulated the expression of mutant p53 in tumor cells at the protein level as well as mRNA level. The same concentration of Pu-erh tea solution did not cause p53 stabilization or activation of its downstream pathways in wild type cells. We also found that Pu-erh tea treatment could slightly down-regulate both HSP70 and HSP90 protein levels in tumor cells. These data revealed the action of Pu-erh tea on tumor cells and provided the possible mechanism for Pu-erh tea action, which explained its selectivity in inhibiting tumor cells without affecting wild type cells. Our data sheds light on the application of Pu-erh tea as a

Design of secure watermarking scheme for watermarking protocol

Watermarking technique enables to hide an imperceptible watermark into a multimedia content for copyright protection. However, in most conventional watermarking schemes, the watermark is embedded solely by the seller, and both the seller and the buyer know the watermarked copy, which causes unsettled dispute at the phase of arbitration. To solve this problem, many watermarking protocols have been proposed using watermarking scheme in the encrypted domain. In this paper, we firstly discuss many security aspects in the encrypted domain, and then propose a new method of homomorphism conversion for probabilistic public key cryptosystem with homomorphic property. Based on our previous work, a new secure watermarking scheme for watermarking protocol is presented using a new embedding strategy in the encrypted domain. We employ an El Gamal variant cryptosystem with additive homomorphic property to reduce the computing overload of watermark embedding in the encrypted domain, and RA code to improve the robustness of the watermarked image against many moderate attacks after decryption. Security analysis and experiment demonstrate that the secure watermarking scheme is more suitable for implementing the existing watermarking protocols.<br /

Effect of microrelief features of tillage methods under different rainfall intensities on runoff and soil erosion in slopes

Tillage methods play a crucial role in controlling rainwater partitioning and soil erosion. This study utilized rainfall simulation experiments to investigate the impact of four tillage methods (manual digging (MD), manual hoeing (MH), traditional ploughing (TP), and ridged ploughing (RP)) on runoff and soil erosion at the plot scale. The smooth slope (SS) was used as a benchmark. Rainfall intensities of 30, 60, 90, and 120 mm h−1 were considered. The study revealed that tillage altered rainwater distribution into depression storage, infiltration, and runoff. Tillage reduces runoff and increases infiltration. The four tillage methods (30–73%) increased the proportion of rainwater converted to infiltration to varying degrees compared to the SS (22–53%). Microrelief features influenced the role of tillage methods in soil erosion. Surface roughness and depression storage accounted for 79% of the variation in sediment yield. The four tillage methods reduced runoff by 2.1–64.7% and sediment yield by 2.5–77.2%. Moreover, increased rainfall intensity weakens the ability of tillage to control soil erosion. When rainfall intensity increased to 120 mm h−1, there was no significant difference in runoff yield among RP, TP, MH, and SS. Therefore, assessing the effectiveness of tillage in reducing soil erosion should consider changes in rainfall intensity. Additionally, the cover management (C) factor of the RUSLE was used to assess the effects of different tillage methods on soil loss. Overall, the C factor values for tilled slopes are in the order MH > TP > RP > MD with a range of 0.23–0.97. As the surface roughness increases, the C factor tends to decrease, and the two are exponential functions (R2 = 0.86). These studies contribute to our understanding of how different tillage methods impact runoff and soil erosion in sloped farmland and provide guidance for selecting appropriate local manual tillage methods

Losartan ameliorates renal fibrosis by inhibiting tumor necrosis factor signal pathway

Losartan is widely used in the treatment of chronic kidney disease (CKD) and has achieved good clinical efficacy, but its exact mechanism is not clear. We performed high-throughput sequencing (HTS) technology to screen the potential target of losartan in treating CKD. According to the HTS results, we found that the tumor necrosis factor (TNF) signal pathway was enriched. Therefore, we conducted in vivo and in vitro experiments to verify it. We found that TNF signal pathway was activated in both unilateral ureteral obstruction (UUO) rats and human proximal renal tubular epithelial cells (HK-2) treated with transforming growth factor-β1 (TGF-β1), while losartan can significantly inhibit TNF signal pathway as well as the expression of fibrosis related genes (such as COL-1, α-SMA and Vimentin). These data suggest that losartan may ameliorate renal fibrosis through modulating the TNF pathway. Resumen: El Losartán es ampliamente utilizado en el tratamiento de la enfermedad renal crónica (CKD) y ha logrado buenos resultados clínicos, pero su mecanismo exacto aún no está claro. Utilizamos la técnica de secuenciación de alto rendimiento (HTS) para detectar posibles dianas de losartán para el tratamiento de la CKD. Según los resultados de HTS, encontramos un enriquecimiento de la vía de señalización del factor de necrosis tumoral (TNF). Así, realizamos experimentos in vivo e in vitro para verificar esto. Encontramos que, tanto en ratas con obstrucción ureteral unilateral (uuo) como en células epiteliales tubulares renales proximal humanas (HK-2) tratadas con factor de crecimiento transformador β1 (TGF-β1), se activó la vía de señalización del TNF. El losartán inhibe significativamente la expresión de las vías de señalización del TNF y genes relacionados con la fibrosis, como COL-1, α-SMA y vicentin. Estos datos sugieren que el losartán puede mejorar la fibrosis renal regulando la vía del TNF