16 research outputs found
The Vehicle, Fall 1993
Table of Contents
7/10ths SynthesisPeter F. Essigpage 5
Aug 1992 (My Small Catechism)Jon Montgomerypage 6
Chaos Is-J. Dylan McNeillpage 7
UntouchedTraci Williamspage 8
The JustificationJohn C. Carminepage 8
LincolnJon Montgomerypage 9
Untitled (Photo)Nicole Niemanpage 10
Park PoemJohn Brillhartpage 11
SmokeJulia Ann Canhampage 12
Warming the BenchAnn Moutraypage 12
Cereal KillerJay Harnackpage 13
The Dutiful SonsTom McGrathpage 14
UntitledCatherine DeGraafpage 17
7-up bottleWalt Howardpage 17
BreedDan Trutterpage 18
An Argument Against LoveTony Martinezpage 19
UntitledT. Scott Laniganpage 19
Glassblowers BallStephanie Franzenpage 20
Portrait of a Young GirlJohn C. Carminepage 20
Untitled (artwork)Dan Trutterpage 21
Death of a FriendLizabeth Kulkapage 22
Submission BluesMartin Paul Brittpage 23
To the Fourteen Year Old SuicideScott Langenpage 23
The Flabby PilgrimTom McGrathpage 24
The Fall of ImmortalityBrian Wheelerpage 25
Merging with AirThom Schnarrepage 26
UntitledCatherine DeGraafpage 27
Tree FishSandra Beauchamppage 28
Country SlumberJ. Dylan McNeillpage 29
Untitled (artwork)Dan Trutterpage 33
Authors\u27 Pagepage 34https://thekeep.eiu.edu/vehicle/1060/thumbnail.jp
An investigation into the effect of cultures of lactic acid bacteria on fermentation in silage. 1. Strain selection
Assembly of Minicellulosomes on the Surface of Bacillus subtilis ▿ †
To cost-efficiently produce biofuels, new methods are needed to convert lignocellulosic biomass into fermentable sugars. One promising approach is to degrade biomass using cellulosomes, which are surface-displayed multicellulase-containing complexes present in cellulolytic Clostridium and Ruminococcus species. In this study we created cellulolytic strains of Bacillus subtilis that display one or more cellulase enzymes. Proteins containing the appropriate cell wall sorting signal are covalently anchored to the peptidoglycan by coexpressing them with the Bacillus anthracis sortase A (SrtA) transpeptidase. This approach was used to covalently attach the Cel8A endoglucanase from Clostridium thermocellum to the cell wall. In addition, a Cel8A-dockerin fusion protein was anchored on the surface of B. subtilis via noncovalent interactions with a cell wall-attached cohesin module. We also demonstrate that it is possible to assemble multienzyme complexes on the cell surface. A three-enzyme-containing minicellulosome was displayed on the cell surface; it consisted of a cell wall-attached scaffoldin protein noncovalently bound to three cellulase-dockerin fusion proteins that were produced in Escherichia coli. B. subtilis has a robust genetic system and is currently used in a wide range of industrial processes. Thus, grafting larger, more elaborate minicellulosomes onto the surface of B. subtilis may yield cellulolytic bacteria with increased potency that can be used to degrade biomass