20 research outputs found

    Development of DNA Based Active Macro–Materials for Biology and Medicine: A Review

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    DNA was first discovered as the carrier of genetic information for the majority of the known living organisms, encoding the secret of life. Its delicate design based upon double helical structure and base pairing offers a stable and reliable media for storing hereditary codes, laying the foundation for the central dogma (Watson et al. 2003). The impact of this molecule is far reaching into scientific community and our society, as manifested in many fields, for instance, forensics (Budowle et al. 2003), besides medicine. To date, a great deal of research effort has been directed towards understandin

    Simultaneous Determination of Young\u27s Modulus, Shear Modulus, and Poisson\u27s Ratio of Soft Hydrogels

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    Besides biological and chemical cues, cellular behavior has been found to be affected by mechanical cues such as traction forces, surface topology, and in particular the mechanical properties of the substrate. The present study focuses on completely characterizing the bulk linear mechanical properties of such soft substrates, a good example of which are hydrogels. The complete characterization involves the measurement of Young\u27s modulus, shear modulus, and Poisson\u27s ratio of these hydrogels, which is achieved by manipulating nonspherical magnetic microneedles embedded inside them. Translating and rotating these microneedles under the influence of a known force or torque, respectively, allows us to determine the local mechanical properties of the hydrogels. Two specific hydrogels, namely bis-cross-linked polyacrylamide gels and DNA cross-linked polyacrylamide gels were used, and their properties were measured as a function of gel concentration. The bis-cross-linked gels were found to have a Poisson\u27s ratio that varied between 0.38 and 0.49, while for the DNA-cross-linked gels, Poisson\u27s ratio varied between 0.36 and 0.49. The local shear moduli, measured on the 10 ÎĽm scale, of these gels were in good agreement with the global shear modulus obtained from a rheology study. Also the local Young\u27s modulus of the hydrogels was compared with the global modulus obtained using bead experiments, and it was observed that the inhomogeneities in the hydrogel increases with increasing cross-linker concentration. This study helps us fully characterize the properties of the substrate, which helps us to better understand the behavior of cells on these substrates
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